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HMGB1 promotes the secretion of multiple cytokines and potentiates the osteogenic differentiation of mesenchymal stem cells through the Ras/MAPK signaling pathway

  • Authors:
    • Lin Feng
    • Deting Xue
    • Erman Chen
    • Wei Zhang
    • Xiang Gao
    • Jiawei Yu
    • Yadong Feng
    • Zhijun Pan
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedics, The First People's Hospital of Xiaoshan, Hangzhou, Zhejiang 311200, P.R. China, Department of Orthopedics, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang 310009, P.R. China
    Copyright: © Feng et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3941-3947
    |
    Published online on: November 2, 2016
       https://doi.org/10.3892/etm.2016.3857
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Abstract

High mobility group box 1 (HMGB1) protein has been previously been detected in the inflammatory microenvironment of bone fractures. It is well known that HMGB1 acts as a chemoattractant to mesenchymal stem cells (MSCs). In the present study, the effects of HMGB1 on cytokine secretion from MSCs were determined, and the molecular mechanisms underlying these effects of HMGB1 on osteogenic differentiation were elucidated. To detect cytokine secretion, antibody array assays were performed, which demonstrated that HGMB1 induced the differential secretion of cytokines that are predominantly associated with cell development, regulation of growth and cell migration, stress responses, and immune system functions. Moreover, the secretion of epidermal growth factor receptor (EGFR) was significantly upregulated by HMGB1. The EGFR‑activated Ras/MAPK pathway regulates the osteogenic differentiation of MSCs. These results suggested that HMGB1 enhances the secretion of various cytokines by MSCs and promotes osteogenic differentiation via the Ras/MAPK signaling pathway. The present study may provide a theoretical basis for the development of novel techniques for the treatment of bone fractures in the future.
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Copy and paste a formatted citation
Spandidos Publications style
Feng L, Xue D, Chen E, Zhang W, Gao X, Yu J, Feng Y and Pan Z: HMGB1 promotes the secretion of multiple cytokines and potentiates the osteogenic differentiation of mesenchymal stem cells through the Ras/MAPK signaling pathway. Exp Ther Med 12: 3941-3947, 2016.
APA
Feng, L., Xue, D., Chen, E., Zhang, W., Gao, X., Yu, J. ... Pan, Z. (2016). HMGB1 promotes the secretion of multiple cytokines and potentiates the osteogenic differentiation of mesenchymal stem cells through the Ras/MAPK signaling pathway. Experimental and Therapeutic Medicine, 12, 3941-3947. https://doi.org/10.3892/etm.2016.3857
MLA
Feng, L., Xue, D., Chen, E., Zhang, W., Gao, X., Yu, J., Feng, Y., Pan, Z."HMGB1 promotes the secretion of multiple cytokines and potentiates the osteogenic differentiation of mesenchymal stem cells through the Ras/MAPK signaling pathway". Experimental and Therapeutic Medicine 12.6 (2016): 3941-3947.
Chicago
Feng, L., Xue, D., Chen, E., Zhang, W., Gao, X., Yu, J., Feng, Y., Pan, Z."HMGB1 promotes the secretion of multiple cytokines and potentiates the osteogenic differentiation of mesenchymal stem cells through the Ras/MAPK signaling pathway". Experimental and Therapeutic Medicine 12, no. 6 (2016): 3941-3947. https://doi.org/10.3892/etm.2016.3857
Copy and paste a formatted citation
x
Spandidos Publications style
Feng L, Xue D, Chen E, Zhang W, Gao X, Yu J, Feng Y and Pan Z: HMGB1 promotes the secretion of multiple cytokines and potentiates the osteogenic differentiation of mesenchymal stem cells through the Ras/MAPK signaling pathway. Exp Ther Med 12: 3941-3947, 2016.
APA
Feng, L., Xue, D., Chen, E., Zhang, W., Gao, X., Yu, J. ... Pan, Z. (2016). HMGB1 promotes the secretion of multiple cytokines and potentiates the osteogenic differentiation of mesenchymal stem cells through the Ras/MAPK signaling pathway. Experimental and Therapeutic Medicine, 12, 3941-3947. https://doi.org/10.3892/etm.2016.3857
MLA
Feng, L., Xue, D., Chen, E., Zhang, W., Gao, X., Yu, J., Feng, Y., Pan, Z."HMGB1 promotes the secretion of multiple cytokines and potentiates the osteogenic differentiation of mesenchymal stem cells through the Ras/MAPK signaling pathway". Experimental and Therapeutic Medicine 12.6 (2016): 3941-3947.
Chicago
Feng, L., Xue, D., Chen, E., Zhang, W., Gao, X., Yu, J., Feng, Y., Pan, Z."HMGB1 promotes the secretion of multiple cytokines and potentiates the osteogenic differentiation of mesenchymal stem cells through the Ras/MAPK signaling pathway". Experimental and Therapeutic Medicine 12, no. 6 (2016): 3941-3947. https://doi.org/10.3892/etm.2016.3857
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