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Article

ARID1A gene silencing reduces the sensitivity of ovarian clear cell carcinoma to cisplatin

  • Authors:
    • Changshuai Lyu
    • Yinglan Zhang
    • Xingnan Zhou
    • Jinghe Lang
  • View Affiliations / Copyright

    Affiliations: Department of Obstetrics and Gynecology, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100730, P.R. China
  • Pages: 4067-4071
    |
    Published online on: November 2, 2016
       https://doi.org/10.3892/etm.2016.3863
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Abstract

In ovarian clear cell carcinoma (OCCC), the mutation rate of the AT-rich interaction domain 1A (ARID1A) gene is 46-57%. However, the effects of ARID1A gene silencing by small interfering RNA (siRNA) on the sensitivity of OCCC to cisplatin have not been investigated. Thus, this study aimed to elucidate the association between ARID1A gene silencing and drug resistance in OCCC. Three pairs of ARID1A gene siRNA fragments (siRNA‑1, siRNA‑2 and siRNA‑3) were designed and transiently transfected into ES2 OCCC cells using RNAi Max reagent. Western blotting results demonstrated that the transfection reduced ARID1A protein expression levels, with the siRNA‑3 group having the lowest levels. The IC50 value, determined using a Cell Counting kit‑8 assay, was significantly increased by siRNA‑3 transfection compared with that in blank control and negative control groups. The cell survival rate following treatment with 50 µM cisplatin for 48 h was significantly increased in the siRNA‑3 group compared with the blank control and negative control groups. Flow cytometric analysis revealed that the apoptosis rate for cisplatin-treated cells was significantly lower in cells with siRNA‑3 transfection than in those without, and the apoptosis rate in siRNA‑3‑transfected cells was lower than that in the negative control group. Western blot analysis showed that the expression level of AKT in cisplatin‑treated cells was significantly decreased compared with that in the negative control group, and the AKT expression level in cisplatin‑treated cells was significantly higher with siRNA‑3 transfection than without. Therefore, the results demonstrated that ARID1A siRNA efficiently decreased ARID1A expression, which reduced cisplatin chemosensitivity and cell apoptosis in ES2 OCCC cells via the regulation of AKT expression.
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Copy and paste a formatted citation
Spandidos Publications style
Lyu C, Zhang Y, Zhou X and Lang J: ARID1A gene silencing reduces the sensitivity of ovarian clear cell carcinoma to cisplatin. Exp Ther Med 12: 4067-4071, 2016.
APA
Lyu, C., Zhang, Y., Zhou, X., & Lang, J. (2016). ARID1A gene silencing reduces the sensitivity of ovarian clear cell carcinoma to cisplatin. Experimental and Therapeutic Medicine, 12, 4067-4071. https://doi.org/10.3892/etm.2016.3863
MLA
Lyu, C., Zhang, Y., Zhou, X., Lang, J."ARID1A gene silencing reduces the sensitivity of ovarian clear cell carcinoma to cisplatin". Experimental and Therapeutic Medicine 12.6 (2016): 4067-4071.
Chicago
Lyu, C., Zhang, Y., Zhou, X., Lang, J."ARID1A gene silencing reduces the sensitivity of ovarian clear cell carcinoma to cisplatin". Experimental and Therapeutic Medicine 12, no. 6 (2016): 4067-4071. https://doi.org/10.3892/etm.2016.3863
Copy and paste a formatted citation
x
Spandidos Publications style
Lyu C, Zhang Y, Zhou X and Lang J: ARID1A gene silencing reduces the sensitivity of ovarian clear cell carcinoma to cisplatin. Exp Ther Med 12: 4067-4071, 2016.
APA
Lyu, C., Zhang, Y., Zhou, X., & Lang, J. (2016). ARID1A gene silencing reduces the sensitivity of ovarian clear cell carcinoma to cisplatin. Experimental and Therapeutic Medicine, 12, 4067-4071. https://doi.org/10.3892/etm.2016.3863
MLA
Lyu, C., Zhang, Y., Zhou, X., Lang, J."ARID1A gene silencing reduces the sensitivity of ovarian clear cell carcinoma to cisplatin". Experimental and Therapeutic Medicine 12.6 (2016): 4067-4071.
Chicago
Lyu, C., Zhang, Y., Zhou, X., Lang, J."ARID1A gene silencing reduces the sensitivity of ovarian clear cell carcinoma to cisplatin". Experimental and Therapeutic Medicine 12, no. 6 (2016): 4067-4071. https://doi.org/10.3892/etm.2016.3863
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