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Article

Protective effect of berberine against oxidative stress-induced apoptosis in rat bone marrow-derived mesenchymal stem cells

  • Authors:
    • Wangyang Li
    • Yamei Liu
    • Bin Wang
    • Yiwen Luo
    • Nianhong Hu
    • Dongfeng Chen
    • Xunchao Zhang
    • Yunpu Xiong
  • View Affiliations / Copyright

    Affiliations: Department of Traumatology, The Third Affiliated Hospital of Guangzhou University of Traditional Chinese Medicine, Guangzhou, Guangdong 510240, P.R. China, Department of Diagnostics of Traditional Chinese Medicine, Guangzhou University of Traditional Chinese Medicine, Guangzhou, Guangdong 510006, P.R. China, Department of Anatomy, Guangzhou University of Traditional Chinese Medicine, Guangzhou, Guangdong 510006, P.R. China
  • Pages: 4041-4048
    |
    Published online on: November 3, 2016
       https://doi.org/10.3892/etm.2016.3866
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Abstract

Bone marrow-derived mesenchymal stem cells (BMSCs) have the potential to be used for the treatment of delayed union, nonunion or persistent bone defects in MSC‑based cell therapy. However, implantation of BMSCs into the fracture site is confronted with apoptosis on account of harsh conditions and oxidative stress. In the present study, the anti‑apoptotic effects of berberine (BBR) on BMSCs subjected to hydrogen peroxide (H2O2) are investigated, and the potential underlying mechanisms are explored. Oxidative injury was induced by exposure to H2O2, and cell viability was assessed using a cell counting kit‑8 assay. The apoptosis of BMSCs was measured by Hoechst 33258 and Annexin V‑fluorescein isothiocyanate/propidium iodide assay. Reactive oxygen species staining and superoxide dismutase (SOD) assay were applied to assess the anti‑oxidative effect of BBR. Finally, western blot was performed to measure the expression levels of phosphorylated (p)‑Akt, B‑cell lymphoma 2 (Bcl‑2), Bcl‑2‑associated X protein (Bax) and cleaved caspase‑3. In the present study, it was identified that BBR remarkably attenuated H2O2‑induced apoptotic cell death via quenching ROS production and increasing SOD activity. Further studies indicated that BBR can reduce apoptosis by upregulating the expression level of p‑Akt and Bcl‑2, and downregulating the expression levels of Bax and cleaved caspase‑3. Taken together, the results of the present study demonstrate that pretreatment with BBR could alleviate H2O2-induced apoptosis in rat BMSCs in vitro.
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Copy and paste a formatted citation
Spandidos Publications style
Li W, Liu Y, Wang B, Luo Y, Hu N, Chen D, Zhang X and Xiong Y: Protective effect of berberine against oxidative stress-induced apoptosis in rat bone marrow-derived mesenchymal stem cells. Exp Ther Med 12: 4041-4048, 2016.
APA
Li, W., Liu, Y., Wang, B., Luo, Y., Hu, N., Chen, D. ... Xiong, Y. (2016). Protective effect of berberine against oxidative stress-induced apoptosis in rat bone marrow-derived mesenchymal stem cells. Experimental and Therapeutic Medicine, 12, 4041-4048. https://doi.org/10.3892/etm.2016.3866
MLA
Li, W., Liu, Y., Wang, B., Luo, Y., Hu, N., Chen, D., Zhang, X., Xiong, Y."Protective effect of berberine against oxidative stress-induced apoptosis in rat bone marrow-derived mesenchymal stem cells". Experimental and Therapeutic Medicine 12.6 (2016): 4041-4048.
Chicago
Li, W., Liu, Y., Wang, B., Luo, Y., Hu, N., Chen, D., Zhang, X., Xiong, Y."Protective effect of berberine against oxidative stress-induced apoptosis in rat bone marrow-derived mesenchymal stem cells". Experimental and Therapeutic Medicine 12, no. 6 (2016): 4041-4048. https://doi.org/10.3892/etm.2016.3866
Copy and paste a formatted citation
x
Spandidos Publications style
Li W, Liu Y, Wang B, Luo Y, Hu N, Chen D, Zhang X and Xiong Y: Protective effect of berberine against oxidative stress-induced apoptosis in rat bone marrow-derived mesenchymal stem cells. Exp Ther Med 12: 4041-4048, 2016.
APA
Li, W., Liu, Y., Wang, B., Luo, Y., Hu, N., Chen, D. ... Xiong, Y. (2016). Protective effect of berberine against oxidative stress-induced apoptosis in rat bone marrow-derived mesenchymal stem cells. Experimental and Therapeutic Medicine, 12, 4041-4048. https://doi.org/10.3892/etm.2016.3866
MLA
Li, W., Liu, Y., Wang, B., Luo, Y., Hu, N., Chen, D., Zhang, X., Xiong, Y."Protective effect of berberine against oxidative stress-induced apoptosis in rat bone marrow-derived mesenchymal stem cells". Experimental and Therapeutic Medicine 12.6 (2016): 4041-4048.
Chicago
Li, W., Liu, Y., Wang, B., Luo, Y., Hu, N., Chen, D., Zhang, X., Xiong, Y."Protective effect of berberine against oxidative stress-induced apoptosis in rat bone marrow-derived mesenchymal stem cells". Experimental and Therapeutic Medicine 12, no. 6 (2016): 4041-4048. https://doi.org/10.3892/etm.2016.3866
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