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Article

Role of miR-145 in chronic constriction injury in rats

  • Authors:
    • Xiaolin Pang
    • Yuanzhang Tang
    • Dongya Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Anesthesiology, First Hospital of Tsinghua University, Beijing 100016, P.R. China, Department of Pain Management, Xuanwu Hospital of Capital Medical University, Beijing 100053, P.R. China
  • Pages: 4121-4127
    |
    Published online on: November 14, 2016
       https://doi.org/10.3892/etm.2016.3900
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Abstract

The present study aims to investigate the effects and underlying mechanisms of miRNA-145 (miR-145) in rat models of chronic constriction injury (CCI). Rats were randomly divided into control, sham, CCI, agomiRNA (agomiR)‑normal control (NC) and agomiR-145 groups (n=25 in each group); in addition, 30 rats with CCI were divided into small hairpin (sh)RNA‑NC and shRNA‑ras responsive element binding protein 1 (RREB1) groups. Paw withdrawal thermal latency (PWTL) and paw withdrawal mechanical threshold (PWMT) were detected. Reverse transcription‑quantitative polymerase chain reaction was used to detect miR‑145 expression levels, and western blotting was performed to measure RREB1 and phosphorylated-protein kinase B (p‑AKT) expression levels. In addition, a dual luciferase reporter assay was conducted to identify the target gene of miR‑145. PWMT and PWTL were decreased in CCI rats and this decrease was alleviated by miR‑145 injection. At 1, 3, 5 and 7 days after CCI, miR‑145 expression level in the spinal cord tissue of rats in the CCI group was significantly decreased compared with 1 day before CCI (P<0.05). Compared with the CCI group, miR‑145 expression level in the agomiR‑145 group was significantly higher (P<0.05). In addition, expression levels of RREB1 and p-AKT were significantly increased in the CCI group and significantly decreased in the agomiR‑145 group (P<0.05). Furthermore, knockdown of RREB1 expression by shRNA‑RREB1 significantly increased values of PWMT and PWTL, decreased expression levels of RREB1 and p‑AKT, and increased miR‑145 expression levels (P<0.05). Further investigation demonstrated that miR‑145 can bind with RREB1 mRNA. In conclusion, miR‑145 may be involved in the development of CCI through regulating the expression of RREB1.
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1 

Gilron I, Baron R and Jensen T: Neuropathic pain: Principles of diagnosis and treatment. Mayo Clin Proc. 90:532–545. 2015. View Article : Google Scholar : PubMed/NCBI

2 

Fontaine D, Blond S, Mertens P and Lanteri-Minet M: Neurosurgical treatment of chronic pain. Neurochirurgie. 61:22–29. 2015. View Article : Google Scholar : PubMed/NCBI

3 

Fallon MT, Storey DJ, Krishan A, Weir CJ, Mitchell R, Fleetwood-Walker SM, Scott AC and Colvin LA: Cancer treatment-related neuropathic pain: Proof of concept study with menthol-a TRPM8 agonist. Support Care Cancer. 23:2769–2777. 2015. View Article : Google Scholar : PubMed/NCBI

4 

Helfert SM, Reimer M, Höper J and Baron R: Individualized pharmacological treatment of neuropathic pain. Clin Pharmacol Ther. 97:135–142. 2015. View Article : Google Scholar : PubMed/NCBI

5 

Rice AS and Smith MT: Angiotensin II type 2-receptor: New clinically validated target in the treatment of neuropathic pain. Clin Pharmacol Ther. 97:128–130. 2015. View Article : Google Scholar : PubMed/NCBI

6 

Jamali Z, Asl Aminabadi N, Attaran R, Pournagiazar F, Oskouei S Ghertasi and Ahmadpour F: MicroRNAs as prognostic molecular signatures in human head and neck squamous cell carcinoma: A systematic review and meta-analysis. Oral Oncol. 51:321–331. 2015. View Article : Google Scholar : PubMed/NCBI

7 

Bienertova-Vasku J, Novak J and Vasku A: MicroRNAs in pulmonary arterial hypertension: Pathogenesis, diagnosis and treatment. J Am Soc Hypertens. 9:221–234. 2015. View Article : Google Scholar : PubMed/NCBI

8 

Greco S, Gorospe M and Martelli F: Noncoding RNA in age-related cardiovascular diseases. J Mol Cell Cardiol. 83:142–155. 2015. View Article : Google Scholar : PubMed/NCBI

9 

Saab BJ and Mansuy IM: Neuroepigenetics of memory formation and impairment: The role of microRNAs. Neuropharmacology. 80:61–69. 2014. View Article : Google Scholar : PubMed/NCBI

10 

Akerblom M and Jakobsson J: MicroRNAs as neuronal fate determinants. Neuroscientist. 20:235–242. 2013. View Article : Google Scholar : PubMed/NCBI

11 

Lutz BM, Bekker A and Tao YX: Noncoding RNAs: New players in chronic pain. Anesthesiology. 121:409–417. 2014. View Article : Google Scholar : PubMed/NCBI

12 

Guo X, Yu L, Chen M, Wu T, Peng X, Guo R and Zhang B: miR-145 mediated the role of aspirin in resisting VSMCs proliferation and anti-inflammation through CD40. J Transl Med. 14:2112016. View Article : Google Scholar : PubMed/NCBI

13 

O'Leary L, Sevinç K, Papazoglou IM, Tildy B, Detillieux K, Halayko AJ, Chung KF and Perry MM: Airway smooth muscle inflammation is regulated by microRNA-145 in COPD. FEBS Lett. 590:1324–1334. 2016. View Article : Google Scholar : PubMed/NCBI

14 

Lu A, Huang Z, Zhang C, Zhang X, Zhao J, Zhang H, Zhang Q, Wu S and Yi X: Differential expression of microRNAs in dorsal root ganglia after sciatic nerve injury. Neural Regen Res. 9:1031–1040. 2014. View Article : Google Scholar : PubMed/NCBI

15 

Norcini M, Sideris A, Hernandez LA Martin, Zhang J, Blanck TJ and Recio-Pinto E: An approach to identify microRNAs involved in neuropathic pain following a peripheral nerve injury. Front Neurosci. 8:2662014. View Article : Google Scholar : PubMed/NCBI

16 

Massi D, Cesinaro AM, Tomasini C, et al: Atypical Spitzoid melanocytic tumors: a morphological, mutational, and FISH analysis. J Am Acad Dermatol. 64:919–935. 2011. View Article : Google Scholar : PubMed/NCBI

17 

Franklin RB, Zou J and Costello LC: The cytotoxic role of RREB1, ZIP3 zinc transporter, and zinc in human pancreatic adenocarcinoma. Cancer Biol Ther. 15:1431–1437. 2014. View Article : Google Scholar : PubMed/NCBI

18 

Hargreaves KM: Capsicum and local anesthetic cocktails for trigeminal pain. Pain. 150:32010. View Article : Google Scholar : PubMed/NCBI

19 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2−ΔΔCt method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

20 

Gilron I, Baron R and Jensen T: Neuropathic pain: Principles of diagnosis and treatment. Mayo Clin Proc. 90:532–545. 2015. View Article : Google Scholar : PubMed/NCBI

21 

Aksoy-Aksel A, Zampa F and Schratt G: MicroRNAs and synaptic plasticity-a mutual relationship. Philos Trans R Soc Lond B Biol Sci. 369:201305152014. View Article : Google Scholar : PubMed/NCBI

22 

Zhang HY, Zheng SJ, Zhao JH, Zhao W, Zheng LF, Zhao D, Li JM, Zhang XF, Chen ZB and Yi XN: MicroRNAs 144, 145, and 214 are down-regulated in primary neurons responding to sciatic nerve transection. Brain Res. 1383:62–70. 2011. View Article : Google Scholar : PubMed/NCBI

23 

Kent OA, Fox-Talbot K and Halushka MK: RREB1 repressed miR-143/145 modulates KRAS signaling through downregulation of multiple targets. Oncogene. 32:2576–2585. 2013. View Article : Google Scholar : PubMed/NCBI

24 

Boufraqech M, Zhang L, Jain M, Patel D, Ellis R, Xiong Y, He M, Nilubol N, Merino MJ and Kebebew E: miR-145 suppresses thyroid cancer growth and metastasis and targets AKT3. Endocr Relat Cancer. 21:517–531. 2014. View Article : Google Scholar : PubMed/NCBI

25 

Noguchi S, Yasui Y, Iwasaki J, Kumazaki M, Yamada N, Naito S and Akao Y: Replacement treatment with microRNA-143 and-145 induces synergistic inhibition of the growth of human bladder cancer cells by regulating PI3K/Akt and MAPK signaling pathways. Cancer Lett. 328:353–361. 2013. View Article : Google Scholar : PubMed/NCBI

26 

Stein AT, Ufret-Vincenty CA, Hua L, Santana LF and Gordon SE: Phosphoinositide 3-kinase binds to TRPV1 and mediates NGF-stimulated TRPV1 trafficking to the plasma membrane. J Gen Physiol. 128:509–522. 2006. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Pang X, Tang Y and Zhang D: Role of miR-145 in chronic constriction injury in rats. Exp Ther Med 12: 4121-4127, 2016.
APA
Pang, X., Tang, Y., & Zhang, D. (2016). Role of miR-145 in chronic constriction injury in rats. Experimental and Therapeutic Medicine, 12, 4121-4127. https://doi.org/10.3892/etm.2016.3900
MLA
Pang, X., Tang, Y., Zhang, D."Role of miR-145 in chronic constriction injury in rats". Experimental and Therapeutic Medicine 12.6 (2016): 4121-4127.
Chicago
Pang, X., Tang, Y., Zhang, D."Role of miR-145 in chronic constriction injury in rats". Experimental and Therapeutic Medicine 12, no. 6 (2016): 4121-4127. https://doi.org/10.3892/etm.2016.3900
Copy and paste a formatted citation
x
Spandidos Publications style
Pang X, Tang Y and Zhang D: Role of miR-145 in chronic constriction injury in rats. Exp Ther Med 12: 4121-4127, 2016.
APA
Pang, X., Tang, Y., & Zhang, D. (2016). Role of miR-145 in chronic constriction injury in rats. Experimental and Therapeutic Medicine, 12, 4121-4127. https://doi.org/10.3892/etm.2016.3900
MLA
Pang, X., Tang, Y., Zhang, D."Role of miR-145 in chronic constriction injury in rats". Experimental and Therapeutic Medicine 12.6 (2016): 4121-4127.
Chicago
Pang, X., Tang, Y., Zhang, D."Role of miR-145 in chronic constriction injury in rats". Experimental and Therapeutic Medicine 12, no. 6 (2016): 4121-4127. https://doi.org/10.3892/etm.2016.3900
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