Effect of glucocorticoid on cytokines TLR9 and TLR7 in peripheral blood for patients with uveitis

Cui,Hong-Pei; Pei,Yu-Xi; Li,Guan-Feng; Lou,Yan-Rui

doi:10.3892/etm.2016.3906

Effect of glucocorticoid on cytokines TLR9 and TLR7 in peripheral blood for patients with uveitis

Authors:
- Hong-Pei Cui
- Yu-Xi Pei
- Guan-Feng Li
- Yan-Rui Lou
View Affiliations

Affiliations: Department of Ophtalmology, Henan Eye Hospital, Henan Eye Institute, Zhengzhou, Henan 450003, P.R. China, Department of Ophtalmology, Women and Infants Hospital of Zhengzhou, Zhengzhou, Henan 450012, P.R. China, Department of Ophtalmology, Children's Hospital of Zhengzhou, Zhengzhou, Henan 450053, P.R. China, Department of Ophtalmology, The Third Affiliated Hospital of Henan University of Traditional Chinese Medicine, Zhengzhou, Henan 450000, P.R. China
Published online on: November 15, 2016 https://doi.org/10.3892/etm.2016.3906
Pages: 3893-3896
Copyright: © Cui et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

The effect of glucocorticoid on cytokines Toll-like receptor (TLR)9 and TLR7 in peripheral blood of patients with uveitis was explored. Forty-six patients with uveitis admitted to our hospital from April 2014 to April 2015 were selected as the research observational group. Thirty-five able‑bodied individuals in the same period were selected as the control group. To treat uveitis, the observational group was injected with glucocorticoid (1-2 mg/kg/day) daily, while the control group did not receive any treatment. The quantity of expression of peripheral blood cytokines TLR9 and TLR7 were detected by the methods of fluorescence quantitative PCR, enzyme-linked immunosorbent assay and western blotting. The content of peripheral blood TLR9 and TLR7 (0.21±0.01, 0.19±0.01) decreased significantly (P<0.05) in observational group after glucocorticoid treatment. Compared with data of control group (0.21±0.01, 0.19±0.01), TLR9 and TLR7 content in peripheral blood after glucocorticoid treatment on the patients with uveitis from observation group (0.19±0.01, 0.17±0.01) did not show any significant difference, for correlation between TLR9 and TLR7 in observation group before and after treatment. It was observed that the cytokine content of TLR9 was associated with TLR7 positively (r=0.653, P=0.012). In conclusion, glucocorticoid can improve uveitis by reducing the content of cytokines TLR9 and TLR7 in peripheral blood.

View Figures

View References

1	Yang Y, Zhang M and Zhuang Z: Causes investigation of uveitis recurrence. Medical J Peking Union Med College Hospital. 4:150–153. 2013.(In Chinese).
2	Xu X and Jiahua Lv: Observation of T lymphocyte change of rat with autoimmunity uveitis. Chinese Ophthalmic Res. 26:841–844. 2008.(In Chinese).
3	Trinh L, Brignole-Baudouin F, Pauly A, Liang H, Houssier M and Baudouin C: Th1- and Th2-related chemokine and chemokine receptor expression on the ocular surface in endotoxin-induced uveitis. Mol Vis. 14:2428–2434. 2008.PubMed/NCBI
4	Thibault DL, Chu AD, Graham KL, Balboni I, Lee LY, Kohlmoos C, Landrigan A, Higgins JP, Tibshirani R and Utz PJ: IRF9 and STAT1 are required for IgG autoantibody production and B cell expression of TLR7 in mice. J Clin Invest. 118:1417–1426. 2008. View Article : Google Scholar : PubMed/NCBI
5	Sun S, Rao NL, Venable J, Thurmond R and Karlsson L: TLR7/9 antagonists as therapeutics for immune-mediated inflammatory disorders. Inflamm Allergy Drug Targets. 6:223–235. 2007. View Article : Google Scholar : PubMed/NCBI
6	Takase H, Yu CR, Ham DI, Chan CC, Chen J, Vistica BP, Wawrousek EF, Durum SK, Egwuagu CE and Gery I: Inflammatory processes triggered by TCR engagement or by local cytokine expression: differences in profiles of gene expression and infiltrating cell populations. J Leukoc Biol. 80:538–545. 2006. View Article : Google Scholar : PubMed/NCBI
7	Wu W: Observation and analysis of glucocorticoid treatment on uveitis in clinical effect. World Latest Medicine Information. 15:94–97. 2015.
8	Jabs DA, Nussenblatt RB and Rosenbaum JT: Standardization of Uveitis Nomenclature (SUN) Working Group: Standardization of uveitis nomenclature for reporting clinical data. Results of the First International Workshop. Am J Ophthalmol. 140:509–516. 2005. View Article : Google Scholar : PubMed/NCBI
9	Sugita S, Kawazoe Y, Imai A, Yamada Y, Horie S and Mochizuki M: Inhibition of Th17 differentiation by anti-TNF-alpha therapy in uveitis patients with Behçet's disease. Arthritis Res Ther. 14:R992012. View Article : Google Scholar : PubMed/NCBI
10	Allensworth JJ, Planck SR, Rosenbaum JT and Rosenzweig HL: Investigation of the differential potentials of TLR agonists to elicit uveitis in mice. J Leukoc Biol. 90:1159–1166. 2011. View Article : Google Scholar : PubMed/NCBI
11	Chinnery HR, Leong CM, Chen W, Forrester JV and McMenamin PG: TLR9 and TLR7/8 activation induces formation of keratic precipitates and giant macrophages in the mouse cornea. J Leukoc Biol. 97:103–110. 2015. View Article : Google Scholar : PubMed/NCBI
12	Yu Z and Liu J: Research summary of related-uveitis cytokines. Heilongjiang Med J. 40:8–9. 2016.(In Chinese).
13	Sun R, Sun L, Bao M, Zhang Y and Wang L, Wu X, Hu D, Liu Y, Yu Y and Wang L: A human microsatellite DNA-mimicking oligodeoxynucleotide with CCT repeats negatively regulates TLR7/9-mediated innate immune responses via selected TLR pathways. Clin Immunol. 134:262–276. 2010. View Article : Google Scholar : PubMed/NCBI
14	Sacco RE, Nicholson TL, Waters TE and Brockmeier SL: Porcine TLR3 characterization and expression in response to influenza virus and Bordetella bronchiseptica. Vet Immunol Immunopathol. 142:57–63. 2011. View Article : Google Scholar : PubMed/NCBI
15	Beutler BA: TLRs and innate immunity. Blood. 113:1399–1407. 2009. View Article : Google Scholar : PubMed/NCBI
16	Hsu LC, Park JM, Zhang K, Luo JL, Maeda S, Kaufman RJ, Eckmann L, Guiney DG and Karin M: The protein kinase PKR is required for macrophage apoptosis after activation of Toll-like receptor 4. Nature. 428:341–345. 2004. View Article : Google Scholar : PubMed/NCBI
17	Krieg AM and Vollmer J: Toll-like receptors 7, 8, and 9: linking innate immunity to autoimmunity. Immunol Rev. 220:251–269. 2007. View Article : Google Scholar : PubMed/NCBI
18	Prinz M, Garbe F, Schmidt H, Mildner A, Gutcher I, Wolter K, Piesche M, Schroers R, Weiss E, Kirschning CJ, et al: Innate immunity mediated by TLR9 modulates pathogenicity in an animal model of multiple sclerosis. J Clin Invest. 116:456–464. 2006. View Article : Google Scholar : PubMed/NCBI
19	Forsbach A, Nemorin JG, Völp K, Samulowitz U, Montino C, Müller C, Tluk S, Hamm S, Bauer S, Lipford GB, et al: Characterization of conserved viral leader RNA sequences that stimulate innate immunity through TLRs. Oligonucleotides. 17:405–417. 2007. View Article : Google Scholar : PubMed/NCBI
20	Hasimu A, Ge L, Li QZ, Zhang RP and Guo X: Expressions of Toll-like receptors 3, 4, 7, and 9 in cervical lesions and their correlation with HPV16 infection in Uighur women. Chin J Cancer. 30:344–350. 2011. View Article : Google Scholar : PubMed/NCBI
21	De Trez C, Pajak B, Brait M, Glaichenhaus N, Urbain J, Moser M, Lauvau G and Muraille E: TLR4 and Toll-IL-1 receptor domain-containing adapter-inducing IFN-beta, but not MyD88, regulate Escherichia coli-induced dendritic cell maturation and apoptosis in vivo. J Immunol. 175:839–846. 2005. View Article : Google Scholar : PubMed/NCBI