Clinicopathological significance of ROCK1 and PIK3CA expression in nasopharyngeal carcinoma

Wang,Xuanyi; Huang,Yuxiang; Guo,Renhua; Liu,Yongbiao; Qian,Yayun; Liu,Dan; Dai,Xiaojun; Wei,Zheng; Jin,Feng; Liu,Yanqing

doi:10.3892/etm.2017.4076

Clinicopathological significance of ROCK1 and PIK3CA expression in nasopharyngeal carcinoma

Authors:
- Xuanyi Wang
- Yuxiang Huang
- Renhua Guo
- Yongbiao Liu
- Yayun Qian
- Dan Liu
- Xiaojun Dai
- Zheng Wei
- Feng Jin
- Yanqing Liu
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Affiliations: Rheumatology Department, Clinical Medical College, Yangzhou University, Yangzhou, Jiangsu 225001, P.R. China, Oncology Department, Clinical Medical College, Yangzhou University, Yangzhou, Jiangsu 225001, P.R. China, Department of Oncology, First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, P.R. China, Department of Radiation Oncology, First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, P.R. China, Institute of Traditional Chinese Medicine and Western Medicine, School of Medicine, Yangzhou University, Yangzhou, Jiangsu 225009, P.R. China, Department of Pathology, Clinical Medical College, Yangzhou University, Yangzhou, Jiangsu 225001, P.R. China
Published online on: January 23, 2017 https://doi.org/10.3892/etm.2017.4076
Pages: 1064-1068

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Abstract

The aim of the present study was to determine the expression of rho-associated coiled‑coil‑containing protein kinase 1 (ROCK1) and phosphatidylinositol-4,5-bisphosphate 3‑kinase catalytic subunit α (PIK3CA) in nasopharyngeal carcinoma (NPC), and to assess the association between the expression of these proteins and the clinicopathological features of NPC. ROCK1 and PIK3CA expressions were assessed in a tissue microarray of sections prepared from the tumors of 81 patients with NPC using immunohistochemistry. Western blot analysis was used to detect ROCK1 and PIK3CA expression in NP69 and 5‑8F cells. χ² analysis revealed that upregulation of ROCK1 was significantly associated with advanced N stage (P=0.032) cancer and increased PIK3CA expression was significantly associated with advanced N stage (P=0.027) and TNM stage (P=0.019) cancer. Furthermore, ROCK1 expression was significantly positively correlated with PIK3CA expression (P=0.01). Western blot analysis demonstrated that levels of ROCK1 (P<0.001) and PIK3CA (P=0.015) were significantly higher in 5‑8F cells compared with NP69 cells. The results of the present study indicate that high levels of ROCK1 and PIK3CA expression may be associated with advanced stages in NPC.

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