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Article

Clinicopathological significance of ROCK1 and PIK3CA expression in nasopharyngeal carcinoma

  • Authors:
    • Xuanyi Wang
    • Yuxiang Huang
    • Renhua Guo
    • Yongbiao Liu
    • Yayun Qian
    • Dan Liu
    • Xiaojun Dai
    • Zheng Wei
    • Feng Jin
    • Yanqing Liu
  • View Affiliations / Copyright

    Affiliations: Rheumatology Department, Clinical Medical College, Yangzhou University, Yangzhou, Jiangsu 225001, P.R. China, Oncology Department, Clinical Medical College, Yangzhou University, Yangzhou, Jiangsu 225001, P.R. China, Department of Oncology, First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, P.R. China, Department of Radiation Oncology, First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, P.R. China, Institute of Traditional Chinese Medicine and Western Medicine, School of Medicine, Yangzhou University, Yangzhou, Jiangsu 225009, P.R. China, Department of Pathology, Clinical Medical College, Yangzhou University, Yangzhou, Jiangsu 225001, P.R. China
  • Pages: 1064-1068
    |
    Published online on: January 23, 2017
       https://doi.org/10.3892/etm.2017.4076
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Abstract

The aim of the present study was to determine the expression of rho-associated coiled‑coil‑containing protein kinase 1 (ROCK1) and phosphatidylinositol-4,5-bisphosphate 3‑kinase catalytic subunit α (PIK3CA) in nasopharyngeal carcinoma (NPC), and to assess the association between the expression of these proteins and the clinicopathological features of NPC. ROCK1 and PIK3CA expressions were assessed in a tissue microarray of sections prepared from the tumors of 81 patients with NPC using immunohistochemistry. Western blot analysis was used to detect ROCK1 and PIK3CA expression in NP69 and 5‑8F cells. χ² analysis revealed that upregulation of ROCK1 was significantly associated with advanced N stage (P=0.032) cancer and increased PIK3CA expression was significantly associated with advanced N stage (P=0.027) and TNM stage (P=0.019) cancer. Furthermore, ROCK1 expression was significantly positively correlated with PIK3CA expression (P=0.01). Western blot analysis demonstrated that levels of ROCK1 (P<0.001) and PIK3CA (P=0.015) were significantly higher in 5‑8F cells compared with NP69 cells. The results of the present study indicate that high levels of ROCK1 and PIK3CA expression may be associated with advanced stages in NPC.
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1 

Chang ET and Adami HO: The enigmatic epidemiology of nasopharyngeal carcinoma. Cancer Epidemiol Biomarkers Prev. 15:1765–1777. 2006. View Article : Google Scholar : PubMed/NCBI

2 

Perri F, Dell'Oca I, Muto P, Schiavone C, Aversa C, Fulciniti F, Solla R, Scarpati GD, Buonerba C, Di Lorenzo G and Caponigro F: Optimal management of a patient with recurrent nasopharyngeal carcinoma. World J Clin Cases. 2:297–300. 2014. View Article : Google Scholar : PubMed/NCBI

3 

Wang T, Riaz N, Cheng S, Lu J and Lee N: Intensity-modulated radiation therapy for nasopharyngeal carcinoma: A review. J Radiat Oncol. 1:129–146. 2012. View Article : Google Scholar

4 

Jin Y, Cai XY, Cai YC, Cao Y, Xia Q, Tan YT, Jiang WQ and Shi YX: To build a prognostic score model containing indispensible tumor markers for metastatic nasopharyngeal carcinoma in an epidemic area. Eur J Cancer. 48:882–888. 2012. View Article : Google Scholar : PubMed/NCBI

5 

Lee AW, Ng WT, Chan YH, Sze H, Chan C and Lam TH: The battle against nasopharyngeal cancer. Radiother Oncol. 104:272–278. 2012. View Article : Google Scholar : PubMed/NCBI

6 

Zhang W, Zeng Z, Wei F, Chen P, Schmitt DC, Fan S, Guo X, Liang F, Shi L, Liu Z, et al: SPLUNC1 is associated with nasopharyngeal carcinoma prognosis and plays an important role in all-trans-retinoic acid-induced growth inhibition and differentiation in nasopharyngeal cancer cells. FEBS J. 281:4815–4829. 2014. View Article : Google Scholar : PubMed/NCBI

7 

Li M, Li C, Li D, Xie Y, Shi J, Li G, Guan Y, Li M, Zhang P, Peng F, et al: Periostin, a stroma-associated protein, correlates with tumor invasiveness and progression in nasopharyngeal carcinoma. Clin Exp Metastasis. 29:865–877. 2012. View Article : Google Scholar : PubMed/NCBI

8 

Newell-Litwa KA, Badoual M, Asmussen H, Patel H, Whitmore L and Horwitz AR: ROCK1 and 2 differentially regulate actomyosin organization to drive cell and synaptic polarity. J Cell Biol. 210:225–242. 2015. View Article : Google Scholar : PubMed/NCBI

9 

Shi Y, Pontrello CG, DeFea KA, Reichardt LF and Ethell IM: Focal adhesion kinase acts downstream of EphB receptors to maintain mature dendritic spines by regulating cofilin activity. J Neurosci. 29:8129–8142. 2009. View Article : Google Scholar : PubMed/NCBI

10 

Lai K, Killingsworth MC and Lee CS: Gene of the month: PIK3CA. J Clin Pathol. 68:253–257. 2015. View Article : Google Scholar : PubMed/NCBI

11 

Zhao Q, Zhang B, Shao Y, Chen L, Wang X, Zhang Z, Shu Y and Guo R: Correlation between the expression levels of miR-1 and PIK3CA in non-small-cell lung cancer and their relationship with clinical characteristics and prognosis. Future Oncol. 10:49–57. 2014. View Article : Google Scholar : PubMed/NCBI

12 

Edge SB, Byrd DR, Compton CC, Fritz AG, Greene F and Trotti A: Pharynx. AJCC Cancer Staging Manual. 7th. Springer Science & Business Media LLC; NY: pp. 41–49. 2010

13 

Bradford MM: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 72:248–254. 1976. View Article : Google Scholar : PubMed/NCBI

14 

Zeng Z, Huang H, Zhang W, Xiang B, Zhou M, Zhou Y, Ma J, Yi M, Li X, Li X, et al: Nasopharyngeal carcinoma: Advances in genomics and molecular genetics. Sci China Life Sci. 54:966–975. 2011. View Article : Google Scholar : PubMed/NCBI

15 

Vigil D, Kim TY, Plachco A, Garton AJ, Castaldo L, Pachter JA, Dong H, Chen X, Tokar B, Campbell SL and Der CJ: ROCK1 and ROCK2 are required for non-small cell lung cancer anchorage-independent growth and invasion. Cancer Res. 72:5338–5347. 2012. View Article : Google Scholar : PubMed/NCBI

16 

Chen J, Ye L, Zhang L and Jiang WG: Placenta growth factor, PLGF, influences the motility of lung cancer cells, the role of Rho associated kinase, Rock1. J Cell Biochem. 105:313–320. 2008. View Article : Google Scholar : PubMed/NCBI

17 

Tripathi V, Popescu NC and Zimonjic DB: DLC1 induces expression of E-cadherin in prostate cancer cells through Rho pathway and suppresses invasion. Oncogene. 33:724–733. 2014. View Article : Google Scholar : PubMed/NCBI

18 

Croft DR, Crighton D, Samuel MS, Lourenco FC, Munro J, Wood J, Bensaad K, Vousden KH, Sansom OJ, Ryan KM and Olson MF: p53-mediated transcriptional regulation and activation of the actin cytoskeleton regulatory RhoC to LIMK2 signaling pathway promotes cell survival. Cell Res. 21:666–682. 2011. View Article : Google Scholar : PubMed/NCBI

19 

Zhang J, He X, Ma Y, Liu Y, Shi H, Guo W and Liu L: Overexpression of ROCK1 and ROCK2 inhibits human laryngeal squamous cell carcinoma. Int J Clin Exp Pathol. 8:244–251. 2015.PubMed/NCBI

20 

Zhang C, Zhang S, Zhang Z, He J, Xu Y and Liu S: ROCK has a crucial role in regulating prostate tumor growth through interaction with c-Myc. Oncogene. 33:5582–5591. 2014. View Article : Google Scholar : PubMed/NCBI

21 

Chen KW, Wang WY, Liang WM, Twu CW, Chao JY, Liang KL, Wu CT, Jiang RS, Shih YT and Lin JC: The volume of retropharyngeal nodes predicts distant metastasis in patients with advanced nasopharyngeal carcinoma. Oral Oncol. 47:1171–1175. 2011. View Article : Google Scholar : PubMed/NCBI

22 

Vivanco I and Sawyers CL: The phosphatidylinositol 3-Kinase AKT pathway in human cancer. Nat Rev Cancer. 2:489–501. 2002. View Article : Google Scholar : PubMed/NCBI

23 

Psyrri A, Seiwert TY and Jimeno A: Molecular pathways in head and neck cancer: EGFR, PI3K and more. Am Soc Clin Oncol Educ Book. 246–255. 2013. View Article : Google Scholar : PubMed/NCBI

24 

Chang YS, Hsu HT, Ko YC, Yeh KT, Chang SJ, Lin CY and Chang JG: Combined mutational analysis of RAS, BRAF, PIK3CA, and TP53 genes in Taiwanese patients with oral squamous cell carcinoma. Oral Surg Oral Med Oral Pathol Oral Radiol. 118:110–116.e1. 2014. View Article : Google Scholar : PubMed/NCBI

25 

Qiu W, Tong GX, Manolidis S, Close LG, Assaad AM and Su GH: Novel mutant-enriched sequencing identified high frequency of PIK3CA mutations in pharyngeal cancer. Int J Cancer. 122:1189–1194. 2008. View Article : Google Scholar : PubMed/NCBI

26 

Barault L, Veyrie N, Jooste V, Lecorre D, Chapusot C, Ferraz JM, Lièvre A, Cortet M, Bouvier AM, Rat P, et al: Mutations in the RAS-MAPK, PI(3)K (phosphatidylinositol-3-OH kinase) signaling network correlate with poor survival in a population-based series of colon cancers. Int J Cancer. 122:2255–2259. 2008. View Article : Google Scholar : PubMed/NCBI

27 

Kalinsky K, Jacks LM, Heguy A, Patil S, Drobnjak M, Bhanot UK, Hedvat CV, Traina TA, Solit D, Gerald W and Moynahan ME: PIK3CA mutation associates with improved outcome in breast cancer. Clin Cancer Res. 15:5049–5059. 2009. View Article : Google Scholar : PubMed/NCBI

28 

Ehrenschwender M, Siegmund D, Wicovsky A, Kracht M, Dittrich-Breiholz O, Spindler V, Waschke J, Kalthoff H, Trauzold A and Wajant H: Mutant PIK3CA licenses TRAIL and CD95L to induce non-apoptotic caspase-8-mediated ROCK activation. Cell Death Differ. 17:1435–1447. 2010. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Wang X, Huang Y, Guo R, Liu Y, Qian Y, Liu D, Dai X, Wei Z, Jin F, Liu Y, Liu Y, et al: Clinicopathological significance of ROCK1 and PIK3CA expression in nasopharyngeal carcinoma. Exp Ther Med 13: 1064-1068, 2017.
APA
Wang, X., Huang, Y., Guo, R., Liu, Y., Qian, Y., Liu, D. ... Liu, Y. (2017). Clinicopathological significance of ROCK1 and PIK3CA expression in nasopharyngeal carcinoma. Experimental and Therapeutic Medicine, 13, 1064-1068. https://doi.org/10.3892/etm.2017.4076
MLA
Wang, X., Huang, Y., Guo, R., Liu, Y., Qian, Y., Liu, D., Dai, X., Wei, Z., Jin, F., Liu, Y."Clinicopathological significance of ROCK1 and PIK3CA expression in nasopharyngeal carcinoma". Experimental and Therapeutic Medicine 13.3 (2017): 1064-1068.
Chicago
Wang, X., Huang, Y., Guo, R., Liu, Y., Qian, Y., Liu, D., Dai, X., Wei, Z., Jin, F., Liu, Y."Clinicopathological significance of ROCK1 and PIK3CA expression in nasopharyngeal carcinoma". Experimental and Therapeutic Medicine 13, no. 3 (2017): 1064-1068. https://doi.org/10.3892/etm.2017.4076
Copy and paste a formatted citation
x
Spandidos Publications style
Wang X, Huang Y, Guo R, Liu Y, Qian Y, Liu D, Dai X, Wei Z, Jin F, Liu Y, Liu Y, et al: Clinicopathological significance of ROCK1 and PIK3CA expression in nasopharyngeal carcinoma. Exp Ther Med 13: 1064-1068, 2017.
APA
Wang, X., Huang, Y., Guo, R., Liu, Y., Qian, Y., Liu, D. ... Liu, Y. (2017). Clinicopathological significance of ROCK1 and PIK3CA expression in nasopharyngeal carcinoma. Experimental and Therapeutic Medicine, 13, 1064-1068. https://doi.org/10.3892/etm.2017.4076
MLA
Wang, X., Huang, Y., Guo, R., Liu, Y., Qian, Y., Liu, D., Dai, X., Wei, Z., Jin, F., Liu, Y."Clinicopathological significance of ROCK1 and PIK3CA expression in nasopharyngeal carcinoma". Experimental and Therapeutic Medicine 13.3 (2017): 1064-1068.
Chicago
Wang, X., Huang, Y., Guo, R., Liu, Y., Qian, Y., Liu, D., Dai, X., Wei, Z., Jin, F., Liu, Y."Clinicopathological significance of ROCK1 and PIK3CA expression in nasopharyngeal carcinoma". Experimental and Therapeutic Medicine 13, no. 3 (2017): 1064-1068. https://doi.org/10.3892/etm.2017.4076
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