Immunohistochemical analysis of microsomal glutathione S‑transferase 1 and clusterin expression in lens epithelial cells of patients with pseudoexfoliation syndrome

Stafiej,Joanna; Hałas‑Wiśniewska,Marta; Izdebska,Magdalena; Gagat,Maciej; Grzanka,Dariusz; Grzanka,Alina; Malukiewicz,Grażyna

doi:10.3892/etm.2017.4085

Immunohistochemical analysis of microsomal glutathione S‑transferase 1 and clusterin expression in lens epithelial cells of patients with pseudoexfoliation syndrome

Authors:
- Joanna Stafiej
- Marta Hałas‑Wiśniewska
- Magdalena Izdebska
- Maciej Gagat
- Dariusz Grzanka
- Alina Grzanka
- Grażyna Malukiewicz
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Affiliations: Department of Ophthalmology, Faculty of Medicine, Nicolaus Copernicus University Collegium Medicum in Bydgoszcz, 85‑094 Bydgoszcz, Poland, Department of Histology and Embryology, Faculty of Medicine, Nicolaus Copernicus University Collegium Medicum in Bydgoszcz, 85‑092 Bydgoszcz, Poland, Department of Dermatology, Sexually Transmitted Diseases and Immunodermatology, Faculty of Medicine, Nicolaus Copernicus University Collegium Medicum in Bydgoszcz, 85‑094 Bydgoszcz, Poland
Published online on: January 24, 2017 https://doi.org/10.3892/etm.2017.4085
Pages: 1057-1063

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Abstract

Pseudoexfoliation syndrome (PEX) is an age‑associated, sight disorder affecting elastic fibers in the eye and visceral organs but its exact etiology remains unknown. The purpose of the current study was to determine the morphology and ultrastructure of lens epithelial cells (LECs), and to use immunohistochemistry to examine localization of microsomal glutathione S‑transferase 1 (MGST1) and clusterin. Anterior lens capsules were obtained from 24 patients (13 PEX and 11 controls) who underwent phacoemulsification. Immunohistochemistry was performed, using antibodies against MGST1 and clusterin, to determine their expression. The morphology and ultrastructure of LECs were evaluated by light and transmission electron microscopy, respectively. The PEX LECs were characterized by significantly lower MGST1 (P=0.0001) and clusterin expression (P=0.0005) compared with the control group patients. PEX LECs were also observed to have significantly increased thickness compared with the control group patients (P=0.0002). The current findings suggest that low MGST1 and clusterin expression levels may be an early clinical indicator of PEX, and that oxidative stress may serve an important role, but that the specific etiology of this disease has yet to be revealed.

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