A micro-CT study of microstructure change of alveolar bone during orthodontic tooth movement under different force magnitudes in rats

An,Jingtao; Li,Ying; Liu,Zhongshuang; Wang,Rui; Zhang,Bin

doi:10.3892/etm.2017.4186

May-2017 Volume 13 Issue 5

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May-2017 Volume 13 Issue 5

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A micro-CT study of microstructure change of alveolar bone during orthodontic tooth movement under different force magnitudes in rats

Authors:
- Jingtao An
- Ying Li
- Zhongshuang Liu
- Rui Wang
- Bin Zhang
View Affiliations / Copyright

Affiliations: Department of Orthodontics, School of Stomatology, Harbin Medical University, Harbin, Heilongjiang 150001, P.R. China, Institute of Hard Tissue Development and Regeneration, The Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150081, P.R. China, Department of Pediatrics, School of Stomatology, Harbin Medical University, Harbin, Heilongjiang 150001, P.R. China

Copyright: © An et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
Pages: 1793-1798
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Published online on: March 6, 2017

https://doi.org/10.3892/etm.2017.4186
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Abstract

The dynamic changes of the microstructure of alveolar bone during orthodontic tooth movement in rats was explored by employing micro-computed tomography (micro-CT) system and to provide theoretical reference for clinical orthodontic treatment. Ten rats were selected randomly as control among 70 adult female Wistar rats, and the other 60 rats were divided into 25-g and 75-g groups of equal number. Orthodontic appliance with force of 25 g and 75 g was installed to perform the molar mesial movement. Microstructural parameters for trabecular bone mesial to the distobuccal root were evaluated at different time points using micro‑CT system. Moreover, distance for mesial movement of the molar were measured. Microstructural parameters for trabecular bone of two groups showed no significant changes from day 0 to day 3 (P>0.05); from day 3 to day 7, bone mineral density (BMD), bone volume/total volume (BV/TV) and trabecular thickness (Tb.Th) decreased significantly (P<0.05), whereas trabecular separation (Tb.Sp) and structure model index (SMI) increased significantly (P<0.05); from day 7 to day 14, in 25‑g group, BMD, BV/TV and Tb.Th increased significantly (P<0.05), while Tb.Sp and SMI decreased significantly (P<0.05). Correspondingly, in 75‑g group, changes of parameters did not carry any statistical significance (P>0.05). Furthermore, the 75‑g group showed larger distance than 25-g group only at day 14 (P<0.05). In conclusion, in order to maintain the health of periodontal tissues, adequate time for repair and recovery is needed to ensure reasonable remolding of alveolar bone and healthy movement of the orthodontic tooth.

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1	Henneman S, Von den Hoff JW and Maltha JC: Mechanobiology of tooth movement. Eur J Orthod. 30:299–306. 2008. View Article : Google Scholar : PubMed/NCBI
2	Wise GE and King GJ: Mechanisms of tooth eruption and orthodontic tooth movement. J Dent Res. 87:414–434. 2008. View Article : Google Scholar : PubMed/NCBI
3	Salazar M, Hernandes L, Ramos AL, Micheletti KR, Albino CC and Cuman Nakamura RK: Effect of teriparatide on induced tooth displacement in ovariectomized rats: A histomorphometric analysis. Am J Orthod Dentofacial Orthop. 139:e337–e344. 2011. View Article : Google Scholar : PubMed/NCBI
4	Martin-Badosa E, Amblard D, Nuzzo S, Elmoutaouakkil A, Vico L and Peyrin F: Excised bone structures in mice: imaging at three-dimensional synchrotron radiation micro CT. Radiology. 229:921–928. 2003. View Article : Google Scholar : PubMed/NCBI
5	Waarsing JH, Day JS, van der Linden JC, Ederveen AG, Spanjers C, De Clerck N, Sasov A, Verhaar JA and Weinans H: Detecting and tracking local changes in the tibiae of individual rats: A novel method to analyse longitudinal in vivo micro-CT data. Bone. 34:163–169. 2004. View Article : Google Scholar : PubMed/NCBI
6	Dempster DW, Compston JE, Drezner MK, Glorieux FH, Kanis JA, Malluche H, Meunier PJ, Ott SM, Recker RR and Parfitt AM: Standardized nomenclature, symbols, and units for bone histomorphometry: a 2012 update of the report of the ASBMR Histomorphometry Nomenclature Committee. J Bone Miner Res. 28:2–17. 2013. View Article : Google Scholar : PubMed/NCBI
7	Parfitt AM, Mathews CH, Villanueva AR, Kleerekoper M, Frame B and Rao DS: Relationships between surface, volume, and thickness of iliac trabecular bone in aging and in osteoporosis. Implications for the microanatomic and cellular mechanisms of bone loss. J Clin Invest. 72:1396–1409. 1983. View Article : Google Scholar : PubMed/NCBI
8	Waldo CM and Rothblatt JM: Histologic response to tooth movement in the laboratory rat; procedure and preliminary observations. J Dent Res. 33:481–486. 1954. View Article : Google Scholar : PubMed/NCBI
9	Ren Y, Maltha JC and Kuijpers-Jagtman AM: The rat as a model for orthodontic tooth movement - a critical review and a proposed solution. Eur J Orthod. 26:483–490. 2004. View Article : Google Scholar : PubMed/NCBI
10	Gonzales C, Hotokezaka H, Arai Y, Ninomiya T, Tominaga J, Jang I, Hotokezaka Y, Tanaka M and Yoshida N: An in vivo 3D micro-CT evaluation of tooth movement after the application of different force magnitudes in rat molar. Angle Orthod. 79:703–714. 2009. View Article : Google Scholar : PubMed/NCBI
11	Xu Y, Zhao T, Xu W and Ding Y: Periodontal microstructure change and tooth movement pattern under different force magnitudes in ovariectomized rats: An in-vivo microcomputed tomography study. Am J Orthod Dentofacial Orthop. 143:828–836. 2013. View Article : Google Scholar : PubMed/NCBI
12	Xu X, Zhou J, Yang F, Wei S and Dai H: Using micro-computed tomography to evaluate the dynamics of orthodontically induced root resorption repair in a rat model. PLoS One. 11:e01501352016. View Article : Google Scholar : PubMed/NCBI
13	Kohno T, Matsumoto Y, Kanno Z, Warita H and Soma K: Experimental tooth movement under light orthodontic forces: rates of tooth movement and changes of the periodontium. J Orthod. 29:129–135. 2002. View Article : Google Scholar : PubMed/NCBI
14	Tomizuka R, Shimizu Y, Kanetaka H, Suzuki A, Urayama S, Kikuchi M, Mitani H and Igarashi K: Histological evaluation of the effects of initially light and gradually increasing force on orthodontic tooth movement. Angle Orthod. 77:410–416. 2007. View Article : Google Scholar : PubMed/NCBI
15	Reitan K and Kvam E: Comparative behavior of human and animal tissue during experimental tooth movement. Angle Orthod. 41:1–14. 1971.PubMed/NCBI
16	Storey E: The nature of tooth movement. Am J Orthod. 63:292–314. 1973. View Article : Google Scholar : PubMed/NCBI
17	King GJ, Keeling SD, McCoy EA and Ward TH: Measuring dental drift and orthodontic tooth movement in response to various initial forces in adult rats. Am J Orthod Dentofacial Orthop. 99:456–465. 1991. View Article : Google Scholar : PubMed/NCBI

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

A micro-CT study of microstructure change of alveolar bone during orthodontic tooth movement under different force magnitudes in rats

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