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Article

Effects of 17β-estradiol and 2-methoxyestradiol on the oxidative stress-hypoxia inducible factor-1 pathway in hypoxic pulmonary hypertensive rats

  • Authors:
    • Li Wang
    • Quan Zheng
    • Yadong Yuan
    • Yanpeng Li
    • Xiaowei Gong
  • View Affiliations / Copyright

    Affiliations: Department of Respiratory Disease and Critical Care Medicine, The Second Hospital of Hebei Medical University, Shijiazhuang, Hebei 050000, P.R. China
  • Pages: 2537-2543
    |
    Published online on: March 20, 2017
       https://doi.org/10.3892/etm.2017.4243
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Abstract

The present study aimed to investigate the effects of 17β-estradiol (E2) and 2-methoxyestradiol (2ME) on the oxidative stress-hypoxia inducible factor-1 (OS-HIF-1) pathway in hypoxic pulmonary hypertensive rats. Female Sprague‑Dawley rats were divided randomly into 4 groups, as follows: i) Control (Group A); ii) ovariectomy (OVX) + hypoxia (Group B); iii) OVX + hypoxia + E2 injection (Group C); and iv) 2ME injection (Group D). The rats were maintained under hypoxic conditions for 8 weeks, and mean pulmonary artery pressure (mPAP) and pulmonary arteriole morphology were measured. The reactive oxygen species, superoxide dismutase (SOD), manganese superoxide dismutase (MnSOD), and copper-zinc superoxide dismutase (Cu/ZnSOD) levels in serum were also measured. MnSOD and HIF‑1α expression levels in lung tissue were determined by western blotting and reverse transcription‑quantitative polymerase chain reaction. The mPAP and arterial remodeling index were significantly elevated following chronic hypoxia exposure; however, experimental data revealed a reduced response in E2 and 2ME intervention rats. Compared with Group A, Group B had significantly elevated oxidative stress levels, as illustrated by increased serum ROS levels, decreased serum SOD and MnSOD levels and decreased MnSOD mRNA and protein expression levels in lung tissue. Furthermore, HIF‑1α mRNA and protein expression in Group B was significantly elevated compared with Group A. E2 and 2ME intervention significantly attenuated the aforementioned parameter changes, suggesting that E2 and 2ME partially ameliorate hypoxic pulmonary hypertension. The underlying mechanism of this may be associated with the increase in MnSOD activity and expression and reduction in ROS level, which reduces the levels of transcription and translation of HIF-1α.
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Copy and paste a formatted citation
Spandidos Publications style
Wang L, Zheng Q, Yuan Y, Li Y and Gong X: Effects of 17β-estradiol and 2-methoxyestradiol on the oxidative stress-hypoxia inducible factor-1 pathway in hypoxic pulmonary hypertensive rats. Exp Ther Med 13: 2537-2543, 2017.
APA
Wang, L., Zheng, Q., Yuan, Y., Li, Y., & Gong, X. (2017). Effects of 17β-estradiol and 2-methoxyestradiol on the oxidative stress-hypoxia inducible factor-1 pathway in hypoxic pulmonary hypertensive rats. Experimental and Therapeutic Medicine, 13, 2537-2543. https://doi.org/10.3892/etm.2017.4243
MLA
Wang, L., Zheng, Q., Yuan, Y., Li, Y., Gong, X."Effects of 17β-estradiol and 2-methoxyestradiol on the oxidative stress-hypoxia inducible factor-1 pathway in hypoxic pulmonary hypertensive rats". Experimental and Therapeutic Medicine 13.5 (2017): 2537-2543.
Chicago
Wang, L., Zheng, Q., Yuan, Y., Li, Y., Gong, X."Effects of 17β-estradiol and 2-methoxyestradiol on the oxidative stress-hypoxia inducible factor-1 pathway in hypoxic pulmonary hypertensive rats". Experimental and Therapeutic Medicine 13, no. 5 (2017): 2537-2543. https://doi.org/10.3892/etm.2017.4243
Copy and paste a formatted citation
x
Spandidos Publications style
Wang L, Zheng Q, Yuan Y, Li Y and Gong X: Effects of 17β-estradiol and 2-methoxyestradiol on the oxidative stress-hypoxia inducible factor-1 pathway in hypoxic pulmonary hypertensive rats. Exp Ther Med 13: 2537-2543, 2017.
APA
Wang, L., Zheng, Q., Yuan, Y., Li, Y., & Gong, X. (2017). Effects of 17β-estradiol and 2-methoxyestradiol on the oxidative stress-hypoxia inducible factor-1 pathway in hypoxic pulmonary hypertensive rats. Experimental and Therapeutic Medicine, 13, 2537-2543. https://doi.org/10.3892/etm.2017.4243
MLA
Wang, L., Zheng, Q., Yuan, Y., Li, Y., Gong, X."Effects of 17β-estradiol and 2-methoxyestradiol on the oxidative stress-hypoxia inducible factor-1 pathway in hypoxic pulmonary hypertensive rats". Experimental and Therapeutic Medicine 13.5 (2017): 2537-2543.
Chicago
Wang, L., Zheng, Q., Yuan, Y., Li, Y., Gong, X."Effects of 17β-estradiol and 2-methoxyestradiol on the oxidative stress-hypoxia inducible factor-1 pathway in hypoxic pulmonary hypertensive rats". Experimental and Therapeutic Medicine 13, no. 5 (2017): 2537-2543. https://doi.org/10.3892/etm.2017.4243
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