Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
May-2017 Volume 13 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
May-2017 Volume 13 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

Use of DNA microarray chips for the rapid detection of Mycobacterium tuberculosis resistance to rifampicin and isoniazid

  • Authors:
    • Peijun Tang
    • Xiafang Wang
    • Xinghua Shen
    • Meihua Shi
    • Xuefeng Zhu
    • Xin Yu
    • Jia Liu
    • Chunhua Ling
    • Meiying Wu
  • View Affiliations / Copyright

    Affiliations: Department of Respiration, The First Hospital Affiliated to Soochow University, Suzhou, Jiangsu 215007, P.R. China, Department of Tuberculosis, The Affiliated Infectious Hospital of Soochow University, The Fifth People's Hospital of Suzhou, Suzhou, Jiangsu 215007, P.R. China
    Copyright: © Tang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2332-2338
    |
    Published online on: March 21, 2017
       https://doi.org/10.3892/etm.2017.4250
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

The objective of the present study was to evaluate the potential development of DNA microarray chips to detect rifampicin (RFP) and isoniazid (INH) resistance in Mycobacterium tuberculosis (MTB), using samples from clinical tuberculosis (TB) patients in Soochow City, China. The sputum samples of 42 patients with TB in the Affiliated Hospital of Infectious Diseases of Soochow University (Soochow, China) were collected. The conventional Lowenstein-Jensen culture medium (Gold Standard) was used to assess drug sensitivity using the absolute concentration method. GeeDom MTB drug detection kits were also used to create a DNA microarray chip and examine the RFP‑resistance associated gene mutation points rpoB-RRDR 511, 513, 516, 526, 531 and 533, and the INH‑resistance associated gene mutation points katG315 and inhA‑15 of the sputum samples. Compared with the results from the absolute concentration method, the susceptibility and specificity of RFP sensitivity detected by the DNA microarray chip were 92.8 and 93.8%, respectively. The susceptibility and specificity of INH sensitivity detected were 66.7 and 81%, respectively. The rpoB-RRDR 526, 531 mutations were the primary causes of MTB RFP resistance and the katG315 mutation was the primary cause of INH resistance. The detection of rpoB and katG gene mutation points by a DNA microarray chip may be used as a rapid, accurate and bulk clinical detection method for RFP and INH resistance in MTB. This is very valuable for the control of TB epidemics.
View Figures

Figure 1

Figure 2

View References

1 

Maimaiti R, Zhang Y, Pan K, Mijiti P, Wubili M, Musa M and Andersson R: High prevalence and low cure rate of tuberculosis among patients with HIV in Xinjiang, China. BMC Infect Dis. 17:152017. View Article : Google Scholar : PubMed/NCBI

2 

Timmins GS and Deretic V: Mechanisms of action of isoniazid. Mol Microbiol. 62:1220–1227. 2006. View Article : Google Scholar : PubMed/NCBI

3 

Ranguelova K, Suarez J, Magliozzo RS and Mason RP: Spin trapping investigation of peroxide-and isoniazid-induced radicals in Mycobacterium tuberculosis catalase-peroxidase. Biochemistry. 47:11377–11385. 2008. View Article : Google Scholar : PubMed/NCBI

4 

World Health Organization (WHO), . Anti-tuberculosis drug resistance in the worldFourth global report. WHO; Geneva: 2008

5 

Yu X, Shen X, Tang P, et al: Analysis of multi-drug resistance of Mycobacterium tuberculosis derived from tuberculosis patients in Suzhou city. J Clin Pulmon Med. 17:269–270. 2012.

6 

Li G: The study of drug sensitivity for mycobacteria, Modern phthisiology. Beijing, PLA Medical Publication; pp. 36–57. 2000

7 

Carbonnelle B and Carpentier E: Bacteriological diagnosis of tuberculosis: Current hieratic classification of methods. Rev Med Interne. 16:518–523. 1995.(In French). View Article : Google Scholar : PubMed/NCBI

8 

Demers AM, Venter A, Friedrich SO, Rojas-Ponce G, Mapamba D, Jugheli L, Sasamalo M, Almeida D, Dorasamy A, Jentsch U, et al: Direct susceptibility testing of Mycobacterium tuberculosis for pyrazinamide by use of the bactec MGIT 960 system. J Clin Microbiol. 54:1276–1281. 2016. View Article : Google Scholar : PubMed/NCBI

9 

Ramaswamy S and Musser JM: Molecular genetic basis of antimicrobial agent resistance in Mycobacterium tuberculosis 1998 update. Tuber Lung Dis. 79:3–29. 1998. View Article : Google Scholar : PubMed/NCBI

10 

Scarpellini P, Braglia S, Carrera P, Cedri M, Cichero P, Colombo A, Crucianelli R, Gori A, Ferrari M and Lazzarin A: Detection of rifampin resistance in Mycobacterium tuberculosis by double gradient-denaturing gradient gel electrophoresis. Antimicrob Agents Chemother. 43:2550–2254. 1999.PubMed/NCBI

11 

Herrera L, Jiménez S, Valverde A, García-Aranda MA and Sáez-Nieto JA: Molecular analysis of rifampicin-resistant Mycobacterium tuberculosis isolated in Spain (1996–2001). Description of new mutations in the rpoB gene and review of the literature. Int J Antimicrob Agents. 21:403–408. 2003. View Article : Google Scholar : PubMed/NCBI

12 

Van Der Zanden AG, TeKoppele-Vije EM, Bhanu N Vijaya, Van Soolingen D and Schouls LM: Use of DNA extracts from Ziehl-Neelsen-stained slides for molecular detection of rifampin resistance and spoligotyping of Mycobacterium tuberculosis. J Clin Microbiol. 41:1101–1118. 2003. View Article : Google Scholar : PubMed/NCBI

13 

Harding CV and Boom WH: Regulation of antigen presentation by Mycobacterium tuberculosis A role for Toll-like receptors. Nat Rev Microbiol. 8:296–307. 2010. View Article : Google Scholar : PubMed/NCBI

14 

Kleinnijenhuis J, Oosting M, Joosten LA, Netea MG and Van Crevel R: Innate immune recognition of Mycobacterium tuberculosis. Clin Dev Immunol. 21:4053102011.

15 

Bostanabad SZ, Nojoumi SA, Jabbarzadeh E, Shekarabei M, Hoseinaei H, Rahimi MK, Ghalami M, Dizji S Pourazar, Sagalchyk ER and Titov LP: High level isoniazid resistance correlates with multiple mutation in the katG encoding catalase proxidase of pulmonary tuberculosis strains from the frontier localities of Iran. Tuberk Toraks. 59:27–35. 2011. View Article : Google Scholar : PubMed/NCBI

16 

Cade CE, Dlouhy AC, Medzihradszky KF, Salas-Castillo SP and Ghiladi RA: Isoniazid-resistanee conferring mutations in Mvcobacterium tuberculosis katG: Catalase, peroxidase, and INH-NADH adduct formaion activities. Protein Sci. 19:458–474. 2010.PubMed/NCBI

17 

Seifert M, Catanzaro D, Catanzaro A and Rodwell TC: Genetic mutations associated with isoniazid resistance in Mycobacterium tuberculosis: A systematic review. PLoS One. 10:e01196282015. View Article : Google Scholar : PubMed/NCBI

18 

Khosravi AD, Goodarzi H and Alavi SM: Detection of genomic mutations in katG, inhA and rpoB genes of Mycobacterium tuberculosis isolates using polymerase chain reaction and multiplex allele-specific polymerase chain reaction. Braz J Infect Dis. 16:57–62. 2012. View Article : Google Scholar : PubMed/NCBI

19 

Lin SY, Hwang SC, Yang YC, Wang CF, Chen YH, Chen TC and Lu PL: Early detection of Mycobacterium tuberculosis complex in BACTEC MGIT cultures using nucleic acid amplification. Eur J Clin Microbiol Infect Dis. 35:977–984. 2016. View Article : Google Scholar : PubMed/NCBI

20 

Al-Rubaye DS, Henihan G, Al-Abasly AK, Seagar AL, Al-Attraqchi AA, Schulze H, Hashim DS, Kamil JK, Laurenson IF and Bachmann TT: Genotypic assessment of drug-resistant tuberculosis in Baghdad and other Iraqi provinces using low-cost and low-density DNA microarrays. J Med Microbiol. 65:114–122. 2016. View Article : Google Scholar : PubMed/NCBI

21 

Ali IF, Babak F, Fazlollah MS and Nematollah JJ: Rapid detection of MDR-Mycobacterium tuberculosis using modified PCR-SSCP from clinical Specimens. Asian Pac J Trop Biomed. 4 Suppl 1:S165–S170. 2014. View Article : Google Scholar : PubMed/NCBI

22 

Linger Y, Kukhtin A, Golova J, Perov A, Lambarqui A, Bryant L, Rudy GB, Dionne K, Fisher SL, Parrish N and Chandler DP: Simplified microarray system for simultaneously detecting rifampin, isoniazid, ethambutol, and streptomycin resistance markers in Mycobacterium tuberculosis. J Clin Microbiol. 52:2100–2107. 2014. View Article : Google Scholar : PubMed/NCBI

23 

Shi XC, Liu XQ, Xie XL, Xu YC and Zhao ZX: Gene chip array for differentiation of mycobacterial species and detection of drug resistance. Chin Med J (Engl). 125:3292–3297. 2012.PubMed/NCBI

24 

Ohno H, Koga H and Kohno S: Multidrug-resistant tuberculosis. 2. Mechanisms of drug-resistance in Mycobacterium tuberculosis-genetic mechanisms of drug-resistance. Kekkaku. 73:657–663. 1998.(In Japanese). PubMed/NCBI

25 

Wang SF, Zhao B, Song YY, Zhou Y, Ou XC, Li Q, Xia H, Pang Y, Duanmu HJ, Fu Y and Zhao YL: Risk factors for drug-resistant tuberculosis in China: analysis of the results of the national drug resistant tuberculosisbaselinesurvey in 2007. Chin J Antituber. 35:221–226. 2013.

26 

van Rie A, Warren R, Mshanga I, Jordaan AM, van der Spuy GD, Richardson M, Simpson J, Gie RP, Enarson DA, Beyers N, et al: Analysis for a limited number of gene codons can predict drug resistance of Mycobacterium tuberculosis in a high-incidence community. J Clin Microbiol. 39:636–641. 2001. View Article : Google Scholar : PubMed/NCBI

27 

de Rossi E, Aínsa JA and Riccardi G: Role of mycobacterial efflux transporters in drug resistance: An unresolved question. FEMS Microbiol Rev. 30:36–52. 2006. View Article : Google Scholar : PubMed/NCBI

28 

Danilchanka O, Mailaender C and Niederweis M: Identification of a novel multidrug efflux pump of Mycobacterium tuberculosis. Antimicrob Agents Chemother. 52:2503–2511. 2008. View Article : Google Scholar : PubMed/NCBI

29 

Heep M, Brandstätter B, Rieger U, Lehn N, Richter E, Rüsch-Gerdes S and Niemann S: Frequency of rpoB mutations inside and outside the cluster I region in rifampin-resistant clinical Mycobacterium tuberculosis strains. J Clin Microbiol. 39:107–110. 2001. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Tang P, Wang X, Shen X, Shi M, Zhu X, Yu X, Liu J, Ling C and Wu M: Use of DNA microarray chips for the rapid detection of Mycobacterium tuberculosis resistance to rifampicin and isoniazid. Exp Ther Med 13: 2332-2338, 2017.
APA
Tang, P., Wang, X., Shen, X., Shi, M., Zhu, X., Yu, X. ... Wu, M. (2017). Use of DNA microarray chips for the rapid detection of Mycobacterium tuberculosis resistance to rifampicin and isoniazid. Experimental and Therapeutic Medicine, 13, 2332-2338. https://doi.org/10.3892/etm.2017.4250
MLA
Tang, P., Wang, X., Shen, X., Shi, M., Zhu, X., Yu, X., Liu, J., Ling, C., Wu, M."Use of DNA microarray chips for the rapid detection of Mycobacterium tuberculosis resistance to rifampicin and isoniazid". Experimental and Therapeutic Medicine 13.5 (2017): 2332-2338.
Chicago
Tang, P., Wang, X., Shen, X., Shi, M., Zhu, X., Yu, X., Liu, J., Ling, C., Wu, M."Use of DNA microarray chips for the rapid detection of Mycobacterium tuberculosis resistance to rifampicin and isoniazid". Experimental and Therapeutic Medicine 13, no. 5 (2017): 2332-2338. https://doi.org/10.3892/etm.2017.4250
Copy and paste a formatted citation
x
Spandidos Publications style
Tang P, Wang X, Shen X, Shi M, Zhu X, Yu X, Liu J, Ling C and Wu M: Use of DNA microarray chips for the rapid detection of Mycobacterium tuberculosis resistance to rifampicin and isoniazid. Exp Ther Med 13: 2332-2338, 2017.
APA
Tang, P., Wang, X., Shen, X., Shi, M., Zhu, X., Yu, X. ... Wu, M. (2017). Use of DNA microarray chips for the rapid detection of Mycobacterium tuberculosis resistance to rifampicin and isoniazid. Experimental and Therapeutic Medicine, 13, 2332-2338. https://doi.org/10.3892/etm.2017.4250
MLA
Tang, P., Wang, X., Shen, X., Shi, M., Zhu, X., Yu, X., Liu, J., Ling, C., Wu, M."Use of DNA microarray chips for the rapid detection of Mycobacterium tuberculosis resistance to rifampicin and isoniazid". Experimental and Therapeutic Medicine 13.5 (2017): 2332-2338.
Chicago
Tang, P., Wang, X., Shen, X., Shi, M., Zhu, X., Yu, X., Liu, J., Ling, C., Wu, M."Use of DNA microarray chips for the rapid detection of Mycobacterium tuberculosis resistance to rifampicin and isoniazid". Experimental and Therapeutic Medicine 13, no. 5 (2017): 2332-2338. https://doi.org/10.3892/etm.2017.4250
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team