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Overexpression of long non‑coding RNA zinc finger antisense 1 in acute myeloid leukemia cell lines influences cell growth and apoptosis

  • Authors:
    • Haifei Guo
    • Lili Wu
    • Pu Zhao
    • Aimei Feng
  • View Affiliations / Copyright

    Affiliations: Department of Hematology, Third Affiliated Hospital of Wenzhou Medical University, Ruian, Zhejiang 325200, P.R. China, Department of Medical Oncology, Third Affiliated Hospital of Wenzhou Medical University, Ruian, Zhejiang 325200, P.R. China
    Copyright: © Guo et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 647-651
    |
    Published online on: June 1, 2017
       https://doi.org/10.3892/etm.2017.4535
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Abstract

The association between long non‑coding RNA zinc finger antisense 1 (ZFAS1) and acute myeloid leukemia (AML) has not yet been investigated. The present study aimed to assess the potential role of ZFAS1 in AML cell proliferation and apoptosis. The expression of ZFAS1 mRNA in various AML cell lines (HL‑60, KG‑1, ML‑1 and SKNO‑1) was measured by reverse transcription‑quantitative polymerase chain reaction. The results showed that ZFAS1 expression was increased in all four human AML cell lines compared with the control cell lines (T lymphocytic leukemia or Burkitt's lymphoma). Transfection with small interfering RNA into human AML cells established ZFAS1 knockdown. A cell‑counting kit‑8 (CCK‑8) assay was used to investigate the effect of ZFAS1 on AML cell proliferation and the effect of ZFAS1 on the cell cycle and cell apoptosis was assessed using flow cytometry. Notably, the CCK‑8 assay demonstrated that ZFAS1 knockdown inhibited cell proliferation in HL‑60 and SKNO‑1 cell lines and flow cytometry analysis indicated that ZFAS1 knockdown induced AML cell cycle G1 phase arrest and triggered cell apoptosis. Therefore, the present study indicated that ZFAS1 promoted the proliferation and inhibited the apoptosis of AML cells.
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Copy and paste a formatted citation
Spandidos Publications style
Guo H, Wu L, Zhao P and Feng A: Overexpression of long non‑coding RNA zinc finger antisense 1 in acute myeloid leukemia cell lines influences cell growth and apoptosis. Exp Ther Med 14: 647-651, 2017.
APA
Guo, H., Wu, L., Zhao, P., & Feng, A. (2017). Overexpression of long non‑coding RNA zinc finger antisense 1 in acute myeloid leukemia cell lines influences cell growth and apoptosis. Experimental and Therapeutic Medicine, 14, 647-651. https://doi.org/10.3892/etm.2017.4535
MLA
Guo, H., Wu, L., Zhao, P., Feng, A."Overexpression of long non‑coding RNA zinc finger antisense 1 in acute myeloid leukemia cell lines influences cell growth and apoptosis". Experimental and Therapeutic Medicine 14.1 (2017): 647-651.
Chicago
Guo, H., Wu, L., Zhao, P., Feng, A."Overexpression of long non‑coding RNA zinc finger antisense 1 in acute myeloid leukemia cell lines influences cell growth and apoptosis". Experimental and Therapeutic Medicine 14, no. 1 (2017): 647-651. https://doi.org/10.3892/etm.2017.4535
Copy and paste a formatted citation
x
Spandidos Publications style
Guo H, Wu L, Zhao P and Feng A: Overexpression of long non‑coding RNA zinc finger antisense 1 in acute myeloid leukemia cell lines influences cell growth and apoptosis. Exp Ther Med 14: 647-651, 2017.
APA
Guo, H., Wu, L., Zhao, P., & Feng, A. (2017). Overexpression of long non‑coding RNA zinc finger antisense 1 in acute myeloid leukemia cell lines influences cell growth and apoptosis. Experimental and Therapeutic Medicine, 14, 647-651. https://doi.org/10.3892/etm.2017.4535
MLA
Guo, H., Wu, L., Zhao, P., Feng, A."Overexpression of long non‑coding RNA zinc finger antisense 1 in acute myeloid leukemia cell lines influences cell growth and apoptosis". Experimental and Therapeutic Medicine 14.1 (2017): 647-651.
Chicago
Guo, H., Wu, L., Zhao, P., Feng, A."Overexpression of long non‑coding RNA zinc finger antisense 1 in acute myeloid leukemia cell lines influences cell growth and apoptosis". Experimental and Therapeutic Medicine 14, no. 1 (2017): 647-651. https://doi.org/10.3892/etm.2017.4535
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