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Mechanism of microRNA-21 regulating IL-6 inflammatory response and cell autophagy in intervertebral disc degeneration

  • Authors:
    • Hao Lin
    • Wenzhi Zhang
    • Tao Zhou
    • Wansu Li
    • Ziao Chen
    • Chaochao Ji
    • Chao Zhang
    • Fang He
  • View Affiliations / Copyright

    Affiliations: Clinical College of Maanshan, Anhui Medical University, Maanshan, Anhui 243000, P.R. China, Department of Orthopaedics, Anhui Provincial Hospital Affiliated to Anhui Medical University, Hefei, Anhui 230000, P.R. China
    Copyright: © Lin et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1441-1444
    |
    Published online on: June 21, 2017
       https://doi.org/10.3892/etm.2017.4637
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Abstract

This study investigated the mechanism of microRNA-21 in regulating IL-6 inflammatory response and cell autophagy in intervertebral disc degeneration. A total of 10 patients with lumbar disc herniation accompanied by nerve root pain (observation group) and 10 patients with lumbar burst fractures (control group) were selected. The nucleus pulposus tissues of the lesion were obtained during operation for cell culture. Real-time quantitative polymerase chain reaction (PCR) was used to detect the expression of microRNA-21.The ELISA method was used to detect the levels of IL-6, and type Ⅱ collagen (Col Ⅱ). Aggrecan and western blotting was used to detect autophagy-related gene 7 (ATG7) and microtubule-associated protein 1 light chain 3 (LC3)-Ⅱ/-Ⅰ. As a result, the levels of microRNA-21 and IL-6 in the observation group were significantly higher than those in the control group, but the levels of Col Ⅱ and aggrecan were significantly lower than those in the control group. The differences were statistically significant (P<0.05). The levels of ATG7 and LC3-Ⅱ/-Ⅰ in the observation group were significantly decreased (P<0.05). In conclusion, the expression of microRNA-21 is abnormally high in the nerve root pain of the lumbar intervertebral disc, which can increase the IL-6 inflammatory response and reduce the capacity of cell autophagy.
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View References

1 

Wang HQ and Samartzis D: Clarifying the nomenclature of intervertebral disc degeneration and displacement: From bench to bedside. Int J Clin Exp Pathol. 7:1293–1298. 2014.PubMed/NCBI

2 

Zhao B, Yu Q, Li H, Guo X and He X: Characterization of microRNA expression profiles in patients with intervertebral disc degeneration. Int J Mol Med. 33:43–50. 2014.PubMed/NCBI

3 

Deng X, Zhao F, Kang B and Zhang X: Elevated interleukin-6 expression levels are associated with intervertebral disc degeneration. Exp Ther Med. 11:1425–1432. 2016.PubMed/NCBI

4 

Risbud MV and Shapiro IM: Role of cytokines in intervertebral disc degeneration: Pain and disc content. Nat Rev Rheumatol. 10:44–56. 2014. View Article : Google Scholar : PubMed/NCBI

5 

Johnson ZI, Schoepflin ZR, Choi H, Shapiro IM and Risbud MV: Disc in flames: Roles of TNF-α and IL-1β in intervertebral disc degeneration. Eur Cell Mater. 30:104–116; discussion 116–117. 2015. View Article : Google Scholar : PubMed/NCBI

6 

Xu K, Chen W, Wang X, Peng Y, Liang A, Huang D, Li C and Ye W: Autophagy attenuates the catabolic effect during inflammatory conditions in nucleus pulposus cells, as sustained by NF-κB and JNK inhibition. Int J Mol Med. 36:661–668. 2015.PubMed/NCBI

7 

Yang Y and Liang C: MicroRNAs: An emerging player in autophagy. ScienceOpen Res. 2015:102015.

8 

Daly C, Ghosh P, Jenkin G, Oehme D and Goldschlager T: A Review of animal models of intervertebral disc degeneration: Pathophysiology, regeneration, and translation to the clinic. BioMed Res Int. 2016:59521652016. View Article : Google Scholar : PubMed/NCBI

9 

Bian Z and Sun J: Development of a KLD-12 polypeptide/TGF-β1-tissue scaffold promoting the differentiation of mesenchymal stem cell into nucleus pulposus-like cells for treatment of intervertebral disc degeneration. Int J Clin Exp Pathol. 8:1093–1103. 2015.PubMed/NCBI

10 

Liu H, Huang X, Liu X, Xiao S, Zhang Y, Xiang T, Shen X, Wang G and Sheng B: miR-21 promotes human nucleus pulposus cell proliferation through PTEN/AKT signaling. Int J Mol Sci. 15:4007–4018. 2014. View Article : Google Scholar : PubMed/NCBI

11 

Chen B, Huang SG, Ju L, Li M, Nie FF, Zhang Y, Zhang YH, Chen X and Gao F: Effect of microRNA-21 on the proliferation of human degenerated nucleus pulposus by targeting programmed cell death 4. Braz J Med Biol Res. 49:e50202016. View Article : Google Scholar

12 

Molinos M, Almeida CR, Caldeira J, Cunha C, Gonçalves RM and Barbosa MA: Inflammation in intervertebral disc degeneration and regeneration. J R Soc Interface. 12:201411912015. View Article : Google Scholar : PubMed/NCBI

13 

Weber KT, Alipui DO, Sison CP, Bloom O, Quraishi S, Overby MC, Levine M and Chahine NO: Serum levels of the proinflammatory cytokine interleukin-6 vary based on diagnoses in individuals with lumbar intervertebral disc diseases. Arthritis Res Ther. 18:32016. View Article : Google Scholar : PubMed/NCBI

14 

Li W, Liu T, Wu L, Chen C, Jia Z, Bai X and Ruan D: Blocking the function of inflammatory cytokines and mediators by using IL-10 and TGF-β: A potential biological immunotherapy for intervertebral disc degeneration in a beagle model. Int J Mol Sci. 15:17270–17283. 2014. View Article : Google Scholar : PubMed/NCBI

15 

Jiang W, Zhang X, Hao J, Shen J, Fang J, Dong W, Wang D, Zhang X, Shui W, Luo Y, et al: SIRT1 protects against apoptosis by promoting autophagy in degenerative human disc nucleus pulposus cells. Sci Rep. 4:74562014. View Article : Google Scholar : PubMed/NCBI

16 

Chen D, Xia D, Pan Z, Xu D, Zhou Y, Wu Y, Cai N, Tang Q, Wang C, Yan M, et al: Metformin protects against apoptosis and senescence in nucleus pulposus cells and ameliorates disc degeneration in vivo. Cell Death Dis. 7:e24412016. View Article : Google Scholar : PubMed/NCBI

17 

Wang XH, Zhu L, Hong X, Wang YT, Wang F, Bao JP, Xie XH, Liu L and Wu XT: Resveratrol attenuated TNF-α-induced MMP-3 expression in human nucleus pulposus cells by activating autophagy via AMPK/SIRT1 signaling pathway. Exp Biol Med (Maywood). 241:848–853. 2016. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Lin H, Zhang W, Zhou T, Li W, Chen Z, Ji C, Zhang C and He F: Mechanism of microRNA-21 regulating IL-6 inflammatory response and cell autophagy in intervertebral disc degeneration. Exp Ther Med 14: 1441-1444, 2017.
APA
Lin, H., Zhang, W., Zhou, T., Li, W., Chen, Z., Ji, C. ... He, F. (2017). Mechanism of microRNA-21 regulating IL-6 inflammatory response and cell autophagy in intervertebral disc degeneration. Experimental and Therapeutic Medicine, 14, 1441-1444. https://doi.org/10.3892/etm.2017.4637
MLA
Lin, H., Zhang, W., Zhou, T., Li, W., Chen, Z., Ji, C., Zhang, C., He, F."Mechanism of microRNA-21 regulating IL-6 inflammatory response and cell autophagy in intervertebral disc degeneration". Experimental and Therapeutic Medicine 14.2 (2017): 1441-1444.
Chicago
Lin, H., Zhang, W., Zhou, T., Li, W., Chen, Z., Ji, C., Zhang, C., He, F."Mechanism of microRNA-21 regulating IL-6 inflammatory response and cell autophagy in intervertebral disc degeneration". Experimental and Therapeutic Medicine 14, no. 2 (2017): 1441-1444. https://doi.org/10.3892/etm.2017.4637
Copy and paste a formatted citation
x
Spandidos Publications style
Lin H, Zhang W, Zhou T, Li W, Chen Z, Ji C, Zhang C and He F: Mechanism of microRNA-21 regulating IL-6 inflammatory response and cell autophagy in intervertebral disc degeneration. Exp Ther Med 14: 1441-1444, 2017.
APA
Lin, H., Zhang, W., Zhou, T., Li, W., Chen, Z., Ji, C. ... He, F. (2017). Mechanism of microRNA-21 regulating IL-6 inflammatory response and cell autophagy in intervertebral disc degeneration. Experimental and Therapeutic Medicine, 14, 1441-1444. https://doi.org/10.3892/etm.2017.4637
MLA
Lin, H., Zhang, W., Zhou, T., Li, W., Chen, Z., Ji, C., Zhang, C., He, F."Mechanism of microRNA-21 regulating IL-6 inflammatory response and cell autophagy in intervertebral disc degeneration". Experimental and Therapeutic Medicine 14.2 (2017): 1441-1444.
Chicago
Lin, H., Zhang, W., Zhou, T., Li, W., Chen, Z., Ji, C., Zhang, C., He, F."Mechanism of microRNA-21 regulating IL-6 inflammatory response and cell autophagy in intervertebral disc degeneration". Experimental and Therapeutic Medicine 14, no. 2 (2017): 1441-1444. https://doi.org/10.3892/etm.2017.4637
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