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CCAAT enhancer binding protein β has a crucial role in regulating breast cancer cell growth via activating the TGF‑β‑Smad3 signaling pathway

  • Authors:
    • Jing Cao
    • Meng Wang
    • Tao Wang
  • View Affiliations / Copyright

    Affiliations: Department of Pharmacy, Linyi People's Hospital of Shandong University, Linyi, Shandong 276000, P.R. China, Department of Opthalmology, Linyi People's Hospital of Shandong University, Linyi, Shandong 276000, P.R. China
    Copyright: © Cao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1554-1560
    |
    Published online on: June 23, 2017
       https://doi.org/10.3892/etm.2017.4659
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Abstract

The aim of the present study was to examine the effect of CCAAT enhancer binding protein β (C/EBPβ) on human breast cancer cells. The plasmids pCDH‑C/EBPβ and pLKO.1‑shC/EBPβ were constructed and were infected into MDA‑MB‑468 cells, to provide C/EBPβ overexpressing and C/EBPβ knockdown cells, respectively. Cell viability, cell cycle and apoptosis were observed by MTT assay and flow cytometry analysis. Protein expression levels of C/EBPβ, TGF‑β1, P‑Smad3 and Smad3 were detected by western blotting. MTT assay showed that the absorbance of MDA‑MB‑468 cells in the pCDH‑C/EBPβ group was increased, whereas that in the pLKO.1‑shC/EBPβ group was decreased, compared with the respective control at 48 and 72 h. Flow cytometric analysis indicated that the percentage of cells in the G2 phase was significantly increased in the pCDH‑C/EBPβ group (P<0.05) and decreased in the pLKO.1‑shC/EBPβ group compared with the respective control group. The proportion of apoptotic cells was decreased in the pCDH‑C/EBPβ group and increased in the pLKO.1‑shC/EBPβ group compared with the controls. The scratch‑wound assay revealed that MDA‑MB‑468 cells depleted of C/EBPβ exhibited reduced motility compared with the control cells. Moreover, western blotting demonstrated that pCDH‑C/EBPβ increased transforming growth factor (TGF)β1 and P‑Smad3 protein expression and decreased Smad3 protein expression, whereas pLKO.1‑shC/EBPβ decreased TGFβ1 and P‑Smad3 protein expression and increased Smad3 protein expression levels. The present study demonstrated that C/EBPβ has a crucial role in regulating breast cancer cell growth through activating TGF‑β‑Smad3 signaling. These findings suggest that C/EBPβ may be a potential therapeutic target for breast cancer; however, in vivo studies are required to confirm this.
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Copy and paste a formatted citation
Spandidos Publications style
Cao J, Wang M and Wang T: CCAAT enhancer binding protein β has a crucial role in regulating breast cancer cell growth via activating the TGF‑β‑Smad3 signaling pathway. Exp Ther Med 14: 1554-1560, 2017.
APA
Cao, J., Wang, M., & Wang, T. (2017). CCAAT enhancer binding protein β has a crucial role in regulating breast cancer cell growth via activating the TGF‑β‑Smad3 signaling pathway. Experimental and Therapeutic Medicine, 14, 1554-1560. https://doi.org/10.3892/etm.2017.4659
MLA
Cao, J., Wang, M., Wang, T."CCAAT enhancer binding protein β has a crucial role in regulating breast cancer cell growth via activating the TGF‑β‑Smad3 signaling pathway". Experimental and Therapeutic Medicine 14.2 (2017): 1554-1560.
Chicago
Cao, J., Wang, M., Wang, T."CCAAT enhancer binding protein β has a crucial role in regulating breast cancer cell growth via activating the TGF‑β‑Smad3 signaling pathway". Experimental and Therapeutic Medicine 14, no. 2 (2017): 1554-1560. https://doi.org/10.3892/etm.2017.4659
Copy and paste a formatted citation
x
Spandidos Publications style
Cao J, Wang M and Wang T: CCAAT enhancer binding protein β has a crucial role in regulating breast cancer cell growth via activating the TGF‑β‑Smad3 signaling pathway. Exp Ther Med 14: 1554-1560, 2017.
APA
Cao, J., Wang, M., & Wang, T. (2017). CCAAT enhancer binding protein β has a crucial role in regulating breast cancer cell growth via activating the TGF‑β‑Smad3 signaling pathway. Experimental and Therapeutic Medicine, 14, 1554-1560. https://doi.org/10.3892/etm.2017.4659
MLA
Cao, J., Wang, M., Wang, T."CCAAT enhancer binding protein β has a crucial role in regulating breast cancer cell growth via activating the TGF‑β‑Smad3 signaling pathway". Experimental and Therapeutic Medicine 14.2 (2017): 1554-1560.
Chicago
Cao, J., Wang, M., Wang, T."CCAAT enhancer binding protein β has a crucial role in regulating breast cancer cell growth via activating the TGF‑β‑Smad3 signaling pathway". Experimental and Therapeutic Medicine 14, no. 2 (2017): 1554-1560. https://doi.org/10.3892/etm.2017.4659
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