Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
August-2017 Volume 14 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
August-2017 Volume 14 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Overexpressed eNOS upregulates SIRT1 expression and protects mouse pancreatic β cells from apoptosis

  • Authors:
    • Tingting Hu
    • Ye Chen
    • Qian Jiang
    • Jun Lin
    • Hewei Li
    • Ping Wang
    • Leping Feng
  • View Affiliations / Copyright

    Affiliations: Department of Biotechnology, Guilin Medical University, Guilin, Guangxi 541004, P.R. China, Graduate School of Guilin Medical University, Guilin, Guangxi 541004, P.R. China, Department of Stomatology, The First Affiliated Hospital of Guilin Medical University, Guilin, Guangxi 541004, P.R. China
  • Pages: 1727-1731
    |
    Published online on: June 26, 2017
       https://doi.org/10.3892/etm.2017.4669
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Loss of sirtuin 1 (SIRT1) activity may be associated with metabolic diseases, including diabetes. The aim of the present study was to investigate the potential effects of overexpressed endothelial nitric oxide synthase (eNOS) on cell proliferation and apoptosis with SIRT1 activation in the Min6 mouse pancreatic β cell line. A pcDNA3.0‑eNOS plasmid was constructed and transfected into Min6 cells for 24 h prior to harvesting. eNOS expression was validated and SIRT1 expression was detected following plasmid transfection using reverse transcription‑quantitative polymerase chain reaction and western blot analysis, which demonstrated that the expression levels of eNOS and SIRT1 were significantly upregulated. Furthermore, the cell proliferation and cell apoptosis of the Min6 cells were evaluated, using a cell counting kit‑8 assay and flow cytometry, respectively. The results suggested that overexpressed eNOS promoted cell proliferation and inhibited cell apoptosis in Min6 cells. The interaction between eNOS and SIRT1 was explored through co‑immunoprecipitation, and it found that there was a strong interaction between eNOS and SIRT1. In conclusion, overexpressed eNOS may induce SIRT1 activation, which is implied to play a protective role in Min6 cells, and eNOS may be a new therapeutic target for diseases such as type 2 diabetes.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

View References

1 

Pernický M, Papinčák J, Reptová A, Kiňová S and Murín J: What may cause diabetes. Vnitr Lek. 61:447–450. 2015.PubMed/NCBI

2 

Linnenkamp U, Guariguata L, Beagley J, Whiting DR and Cho NH: The IDF Diabetes Atlas methodology for estimating global prevalence of hyperglycaemia in pregnancy. Diabetes Res Clin Pract. 103:186–196. 2014. View Article : Google Scholar : PubMed/NCBI

3 

Chan JC, Cho NH, Tajima N and Shaw J: Diabetes in the Western Pacific Region - past, present and future. Diabetes Res Clin Pract. 103:244–255. 2014. View Article : Google Scholar : PubMed/NCBI

4 

IDF Diabetes Atlas Group, . Update of mortality attributable to diabetes for the IDF Diabetes Atlas: Estimates for the year 2013. Diabetes Res Clin Pract. 109:461–465. 2015. View Article : Google Scholar : PubMed/NCBI

5 

Codocedo JF, Allard C, Godoy JA, Varela-Nallar L and Inestrosa NC: SIRT1 regulates dendritic development in hippocampal neurons. PLoS One. 7:e470732012. View Article : Google Scholar : PubMed/NCBI

6 

Kitada M and Koya D: SIRT1 in type 2 diabetes: Mechanisms and therapeutic potential. Diabetes Metab J. 37:315–325. 2013. View Article : Google Scholar : PubMed/NCBI

7 

Kitada M, Kume S, Kanasaki K, Takeda-Watanabe A and Koya D: Sirtuins as possible drug targets in type 2 diabetes. Curr Drug Targets. 14:622–636. 2013. View Article : Google Scholar : PubMed/NCBI

8 

Ota H, Eto M, Ogawa S, Iijima K, Akishita M and Ouchi Y: SIRT1/eNOS axis as a potential target against vascular senescence, dysfunction and atherosclerosis. J Atheroscler Thromb. 17:431–435. 2010. View Article : Google Scholar : PubMed/NCBI

9 

Orimo M, Minamino T, Miyauchi H, Tateno K, Okada S, Moriya J and Komuro I: Protective role of SIRT1 in diabetic vascular dysfunction. Arterioscler Thromb Vasc Biol. 29:889–894. 2009. View Article : Google Scholar : PubMed/NCBI

10 

Shiota A, Shimabukuro M, Fukuda D, Soeki T, Sato H, Uematsu E, Hirata Y, Kurobe H, Maeda N, Sakaue H, et al: Telmisartan ameliorates insulin sensitivity by activating the AMPK/SIRT1 pathway in skeletal muscle of obese db/db mice. Cardiovasc Diabetol. 11:1392012. View Article : Google Scholar : PubMed/NCBI

11 

Ding M, Lei J, Han H, Li W, Qu Y, Fu E, Fu F and Wang X: SIRT1 protects against myocardial ischemia-reperfusion injury via activating eNOS in diabetic rats. Cardiovasc Diabetol. 14:1432015. View Article : Google Scholar : PubMed/NCBI

12 

Lempiainen J, Finckenberg P, Mervaala EE, Sankari S, Levijoki J and Mervaala EM: Caloric restriction ameliorates kidney ischaemia/reperfusion injury through PGC-1α-eNOS pathway and enhanced autophagy. Acta Physiol (Oxf). 208:410–421. 2013. View Article : Google Scholar : PubMed/NCBI

13 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C(T)) Method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

14 

Yan X, Hao Q, Mu Y, Timani KA, Ye L, Zhu Y and Wu J: Nucleocapsid protein of SARS-CoV activates the expression of cyclooxygenase-2 by binding directly to regulatory elements for nuclear factor-kappa B and CCAAT/enhancer binding protein. Int J Biochem Cell Biol. 38:1417–1428. 2006. View Article : Google Scholar : PubMed/NCBI

15 

Alessi DR, Cuenda A, Cohen P, Dudley DT and Saltiel AR: PD 098059 is a specific inhibitor of the activation of mitogen-activated protein kinase kinase in vitro and in vivo. J Biol Chem. 270:27489–27494. 1995. View Article : Google Scholar : PubMed/NCBI

16 

Mohr T, Van Soeren M, Graham TE and Kjaer M: Caffeine ingestion and metabolic responses of tetraplegic humans during electrical cycling. J Appl Physiol (1985). 85:979–985. 1998.PubMed/NCBI

17 

Eghbalzadeh K, Brixius K, Bloch W and Brinkmann C: Skeletal muscle nitric oxide (NO) synthases and NO-signaling in ‘diabesity’-what about the relevance of exercise training interventions? Nitric Oxide. 37:28–40. 2014. View Article : Google Scholar : PubMed/NCBI

18 

Strawn WB: Pathophysiological and clinical implications of AT(1) and AT(2) angiotensin II receptors in metabolic disorders: Hypercholesterolaemia and diabetes. Drugs 62. 1:31–41. 2002.(In French). View Article : Google Scholar

19 

Schafer A and Bauersachs J: Endothelial dysfunction, impaired endogenous platelet inhibition and platelet activation in diabetes and atherosclerosis. Curr Vasc Pharmacol. 6:52–60. 2008. View Article : Google Scholar : PubMed/NCBI

20 

Monti M, Solito R, Puccetti L, Pasotti L, Roggeri R, Monzani E, Casella L and Morbidelli L: Protective effects of novel metal-nonoates on the cellular components of the vascular system. J Pharmacol Exp Ther. 351:500–509. 2014. View Article : Google Scholar : PubMed/NCBI

21 

Paschalaki KE, Starke RD, Hu Y, Mercado N, Margariti A, Gorgoulis VG, Randi AM and Barnes PJ: Dysfunction of endothelial progenitor cells from smokers and chronic obstructive pulmonary disease patients due to increased DNA damage and senescence. Stem Cells. 31:2813–2826. 2013. View Article : Google Scholar : PubMed/NCBI

22 

Lemarie CA, Shbat L, Marchesi C, Angulo OJ, Deschênes ME, Blostein MD, Paradis P and Schiffrin EL: Mthfr deficiency induces endothelial progenitor cell senescence via uncoupling of eNOS and downregulation of SIRT1. Am J Physiol Heart Circ Physiol. 300:H745–H753. 2011. View Article : Google Scholar : PubMed/NCBI

23 

Ota H, Akishita M, Akiyoshi T, Kahyo T, Setou M, Ogawa S, Iijima K, Eto M and Ouchi Y: Testosterone deficiency accelerates neuronal and vascular aging of SAMP8 mice: Protective role of eNOS and SIRT1. PLoS One. 7:e295982012. View Article : Google Scholar : PubMed/NCBI

24 

Tajbakhsh S, Aliakbari K, Hussey DJ, Lower KM, Donato AJ and Sokoya EM: Differential telomere shortening in blood versus arteries in an animal model of type 2 diabetes. J Diabetes Res. 2015:1538292015. View Article : Google Scholar : PubMed/NCBI

25 

Guo R, Liu W, Liu B, Zhang B, Li W and Xu Y: SIRT1 suppresses cardiomyocyte apoptosis in diabetic cardiomyopathy: An insight into endoplasmic reticulum stress response mechanism. Int J Cardiol. 191:36–45. 2015. View Article : Google Scholar : PubMed/NCBI

26 

Gencoglu H, Tuzcu M, Hayirli A and Sahin K: Protective effects of resveratrol against streptozotocin-induced diabetes in rats by modulation of visfatin/sirtuin-1 pathway and glucose transporters. Int J Food Sci Nutr. 66:314–320. 2015. View Article : Google Scholar : PubMed/NCBI

27 

Tajbakhsh N and Sokoya EM: Sirtuin 1 is upregulated in young obese Zucker rat cerebral arteries. Eur J Pharmacol. 721:43–48. 2013. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Hu T, Chen Y, Jiang Q, Lin J, Li H, Wang P and Feng L: Overexpressed eNOS upregulates SIRT1 expression and protects mouse pancreatic β cells from apoptosis. Exp Ther Med 14: 1727-1731, 2017.
APA
Hu, T., Chen, Y., Jiang, Q., Lin, J., Li, H., Wang, P., & Feng, L. (2017). Overexpressed eNOS upregulates SIRT1 expression and protects mouse pancreatic β cells from apoptosis. Experimental and Therapeutic Medicine, 14, 1727-1731. https://doi.org/10.3892/etm.2017.4669
MLA
Hu, T., Chen, Y., Jiang, Q., Lin, J., Li, H., Wang, P., Feng, L."Overexpressed eNOS upregulates SIRT1 expression and protects mouse pancreatic β cells from apoptosis". Experimental and Therapeutic Medicine 14.2 (2017): 1727-1731.
Chicago
Hu, T., Chen, Y., Jiang, Q., Lin, J., Li, H., Wang, P., Feng, L."Overexpressed eNOS upregulates SIRT1 expression and protects mouse pancreatic β cells from apoptosis". Experimental and Therapeutic Medicine 14, no. 2 (2017): 1727-1731. https://doi.org/10.3892/etm.2017.4669
Copy and paste a formatted citation
x
Spandidos Publications style
Hu T, Chen Y, Jiang Q, Lin J, Li H, Wang P and Feng L: Overexpressed eNOS upregulates SIRT1 expression and protects mouse pancreatic β cells from apoptosis. Exp Ther Med 14: 1727-1731, 2017.
APA
Hu, T., Chen, Y., Jiang, Q., Lin, J., Li, H., Wang, P., & Feng, L. (2017). Overexpressed eNOS upregulates SIRT1 expression and protects mouse pancreatic β cells from apoptosis. Experimental and Therapeutic Medicine, 14, 1727-1731. https://doi.org/10.3892/etm.2017.4669
MLA
Hu, T., Chen, Y., Jiang, Q., Lin, J., Li, H., Wang, P., Feng, L."Overexpressed eNOS upregulates SIRT1 expression and protects mouse pancreatic β cells from apoptosis". Experimental and Therapeutic Medicine 14.2 (2017): 1727-1731.
Chicago
Hu, T., Chen, Y., Jiang, Q., Lin, J., Li, H., Wang, P., Feng, L."Overexpressed eNOS upregulates SIRT1 expression and protects mouse pancreatic β cells from apoptosis". Experimental and Therapeutic Medicine 14, no. 2 (2017): 1727-1731. https://doi.org/10.3892/etm.2017.4669
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team