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Article

Abnormal phenotypic features of IgM+B cell subsets in patients with chronic hepatitis C virus infection

  • Authors:
    • Fanyun Kong
    • Bo Feng
    • Henghui Zhang
    • Huiying Rao
    • Jianghua Wang
    • Xu Cong
    • Lai Wei
  • View Affiliations / Copyright

    Affiliations: Beijing Key Laboratory for Hepatitis C and Immunotherapy for Liver Disease, Peking University People's Hospital, Peking University Hepatology Institute, Beijing 100044, P.R. China
  • Pages: 1846-1852
    |
    Published online on: June 27, 2017
       https://doi.org/10.3892/etm.2017.4682
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Abstract

Hepatitis C virus (HCV) infection is associated with B cell abnormality; however the phenotypic profiles of immunoglobulin (Ig)M+B cell subsets in patients with HCV infection remain unclear. In the current study, the effect of HCV infection on IgM+B cell subsets was evaluated. The percentages, as well as the differentiation and activation features of peripheral IgM+B naive subsets [cluster of differentiation (CD)27‑IgM+B cells] and IgM+B memory subsets (CD27+IgM+B cells) were assessed using flow cytometry in 27 patients with chronic hepatitis C (CHC) and 20 healthy controls (HCs). The frequency of CD27+IgM+B memory subsets detected in patients with CHC was significantly higher than that in HCs (P<0.05). Although the frequency of CD27‑IgM+B naive subsets was similar in both groups, there was a significantly higher proportion of CD5+B cells detected in the CD27‑IgM+B subsets of patients with CHC compared with HCs (P<0.05). Among CD27‑IgM+B subsets, abnormal differentiation was associated with HCV infection, with significantly increased percentages of IgD+B cells and CD38+B cells in patients with CHC compared with HCs (P<0.05). In CD27+IgM+B memory subsets, the abnormality of cell differentiation was associated with a significantly increased percentage of CD38+B cells in patients with CHC compared with HCs (P<0.05). In addition, the percentage of activated CD27+IgM+B subsets in patients with CHC were significantly higher than those observed in HCs (P<0.05). The number of CD27‑IgD+IgM+B, CD27‑CD38+IgM+B and CD27+CD38+IgM+B cells were negatively correlated with HCV RNA in patients with CHC. These results suggest that HCV infection contributes to abnormalities in the percentage, differentiation and activation of IgM+B cell subsets and may disrupt the immune response mediated by IgM+B cells.
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Copy and paste a formatted citation
Spandidos Publications style
Kong F, Feng B, Zhang H, Rao H, Wang J, Cong X and Wei L: Abnormal phenotypic features of IgM+B cell subsets in patients with chronic hepatitis C virus infection. Exp Ther Med 14: 1846-1852, 2017.
APA
Kong, F., Feng, B., Zhang, H., Rao, H., Wang, J., Cong, X., & Wei, L. (2017). Abnormal phenotypic features of IgM+B cell subsets in patients with chronic hepatitis C virus infection. Experimental and Therapeutic Medicine, 14, 1846-1852. https://doi.org/10.3892/etm.2017.4682
MLA
Kong, F., Feng, B., Zhang, H., Rao, H., Wang, J., Cong, X., Wei, L."Abnormal phenotypic features of IgM+B cell subsets in patients with chronic hepatitis C virus infection". Experimental and Therapeutic Medicine 14.2 (2017): 1846-1852.
Chicago
Kong, F., Feng, B., Zhang, H., Rao, H., Wang, J., Cong, X., Wei, L."Abnormal phenotypic features of IgM+B cell subsets in patients with chronic hepatitis C virus infection". Experimental and Therapeutic Medicine 14, no. 2 (2017): 1846-1852. https://doi.org/10.3892/etm.2017.4682
Copy and paste a formatted citation
x
Spandidos Publications style
Kong F, Feng B, Zhang H, Rao H, Wang J, Cong X and Wei L: Abnormal phenotypic features of IgM+B cell subsets in patients with chronic hepatitis C virus infection. Exp Ther Med 14: 1846-1852, 2017.
APA
Kong, F., Feng, B., Zhang, H., Rao, H., Wang, J., Cong, X., & Wei, L. (2017). Abnormal phenotypic features of IgM+B cell subsets in patients with chronic hepatitis C virus infection. Experimental and Therapeutic Medicine, 14, 1846-1852. https://doi.org/10.3892/etm.2017.4682
MLA
Kong, F., Feng, B., Zhang, H., Rao, H., Wang, J., Cong, X., Wei, L."Abnormal phenotypic features of IgM+B cell subsets in patients with chronic hepatitis C virus infection". Experimental and Therapeutic Medicine 14.2 (2017): 1846-1852.
Chicago
Kong, F., Feng, B., Zhang, H., Rao, H., Wang, J., Cong, X., Wei, L."Abnormal phenotypic features of IgM+B cell subsets in patients with chronic hepatitis C virus infection". Experimental and Therapeutic Medicine 14, no. 2 (2017): 1846-1852. https://doi.org/10.3892/etm.2017.4682
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