miR-127-5p promotes chondrogenic differentiation in rat bone marrow mesenchymal stem cells

Xue,Zhaolong; Meng,Yanli; Ge,Jianhua

doi:10.3892/etm.2017.4711

miR-127-5p promotes chondrogenic differentiation in rat bone marrow mesenchymal stem cells

Authors:
- Zhaolong Xue
- Yanli Meng
- Jianhua Ge
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Affiliations: Department of Orthopedics, Shanghai Jiading Nanxiang Hospital, Shanghai, P.R. China, Department of Bone and Joint Surgery, The Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan, P.R. China
Published online on: June 28, 2017 https://doi.org/10.3892/etm.2017.4711
Pages: 1481-1486
Copyright: © Xue et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The effect and related mechanisms of miR-127-5p on the cartilage differentiation of rat bone marrow mesenchymal stem cells (BMSCs) was investigated. Rat BMSCs were generated and transfected with miR-127-5p, RT-PCR and Safranin O staining were used to detect the effect of miR-127-5p on the cartilage differentiation of rat BMSCs. Western blot analysis was used to detect the related mechanisms of miR-127-5p on the cartilage differentiation of rat BMSCs. Genes related to cartilage differentiation such as Sox9, collagen Ⅱ and aggrecan were significantly increased in the group which were transfected with miR-127-5p, while collagen X, which was related to cartilage hypertrophy, was decreased in the miR-127-5p transfected group. Safranin O staining revealed that the expression of chondroitin sulfate was significantly increased in the group of miR-127-5p, than the miRNA control group. Western blot analysis showed that miR-127-5p transfection promoted the expression of Sox9, while decreased the expression of Runx2 of rat BMSCs. In conclusion, via increasing the expression of Sox9 and decreasing the expression of Runx2, miR-127-5p could promote cartilage differentiation and decrease cartilage hypertrophy of rat BMSCs.

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