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miR-127-5p promotes chondrogenic differentiation in rat bone marrow mesenchymal stem cells

  • Authors:
    • Zhaolong Xue
    • Yanli Meng
    • Jianhua Ge
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedics, Shanghai Jiading Nanxiang Hospital, Shanghai, P.R. China, Department of Bone and Joint Surgery, The Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan, P.R. China
    Copyright: © Xue et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1481-1486
    |
    Published online on: June 28, 2017
       https://doi.org/10.3892/etm.2017.4711
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Abstract

The effect and related mechanisms of miR-127-5p on the cartilage differentiation of rat bone marrow mesenchymal stem cells (BMSCs) was investigated. Rat BMSCs were generated and transfected with miR-127-5p, RT-PCR and Safranin O staining were used to detect the effect of miR-127-5p on the cartilage differentiation of rat BMSCs. Western blot analysis was used to detect the related mechanisms of miR-127-5p on the cartilage differentiation of rat BMSCs. Genes related to cartilage differentiation such as Sox9, collagen Ⅱ and aggrecan were significantly increased in the group which were transfected with miR-127-5p, while collagen X, which was related to cartilage hypertrophy, was decreased in the miR-127-5p transfected group. Safranin O staining revealed that the expression of chondroitin sulfate was significantly increased in the group of miR-127-5p, than the miRNA control group. Western blot analysis showed that miR-127-5p transfection promoted the expression of Sox9, while decreased the expression of Runx2 of rat BMSCs. In conclusion, via increasing the expression of Sox9 and decreasing the expression of Runx2, miR-127-5p could promote cartilage differentiation and decrease cartilage hypertrophy of rat BMSCs.
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Copy and paste a formatted citation
Spandidos Publications style
Xue Z, Meng Y and Ge J: miR-127-5p promotes chondrogenic differentiation in rat bone marrow mesenchymal stem cells. Exp Ther Med 14: 1481-1486, 2017.
APA
Xue, Z., Meng, Y., & Ge, J. (2017). miR-127-5p promotes chondrogenic differentiation in rat bone marrow mesenchymal stem cells. Experimental and Therapeutic Medicine, 14, 1481-1486. https://doi.org/10.3892/etm.2017.4711
MLA
Xue, Z., Meng, Y., Ge, J."miR-127-5p promotes chondrogenic differentiation in rat bone marrow mesenchymal stem cells". Experimental and Therapeutic Medicine 14.2 (2017): 1481-1486.
Chicago
Xue, Z., Meng, Y., Ge, J."miR-127-5p promotes chondrogenic differentiation in rat bone marrow mesenchymal stem cells". Experimental and Therapeutic Medicine 14, no. 2 (2017): 1481-1486. https://doi.org/10.3892/etm.2017.4711
Copy and paste a formatted citation
x
Spandidos Publications style
Xue Z, Meng Y and Ge J: miR-127-5p promotes chondrogenic differentiation in rat bone marrow mesenchymal stem cells. Exp Ther Med 14: 1481-1486, 2017.
APA
Xue, Z., Meng, Y., & Ge, J. (2017). miR-127-5p promotes chondrogenic differentiation in rat bone marrow mesenchymal stem cells. Experimental and Therapeutic Medicine, 14, 1481-1486. https://doi.org/10.3892/etm.2017.4711
MLA
Xue, Z., Meng, Y., Ge, J."miR-127-5p promotes chondrogenic differentiation in rat bone marrow mesenchymal stem cells". Experimental and Therapeutic Medicine 14.2 (2017): 1481-1486.
Chicago
Xue, Z., Meng, Y., Ge, J."miR-127-5p promotes chondrogenic differentiation in rat bone marrow mesenchymal stem cells". Experimental and Therapeutic Medicine 14, no. 2 (2017): 1481-1486. https://doi.org/10.3892/etm.2017.4711
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