DL‑3‑n‑butylphthalide protects endothelial cells against advanced glycation end product‑induced injury by attenuating oxidative stress and inflammation responses

  • Authors:
    • Chang‑Yun Liu
    • Zhen‑Hua Zhao
    • Zhi‑Ting Chen
    • Chun‑Hui Che
    • Zhang‑Yu Zou
    • Xiao‑Min Wu
    • Sheng‑Gen Chen
    • Yuan‑Xiao Li
    • Han‑Bin Lin
    • Xiao‑Fan Wei
    • Jie You
    • Hua‑Pin Huang
  • View Affiliations

  • Published online on: July 12, 2017     https://doi.org/10.3892/etm.2017.4784
  • Pages: 2241-2248
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Abstract

Endothelial dysfunction, regarded as a key step in the pathophysiological course of diabetic vascular complications, is initiated and deteriorated by advanced glycation end products (AGEs). DL‑3‑n‑butylphthalide (DL‑NBP) has been proven to have protective effects on neurons and vascular endothelial cells against ischemic and anoxic damage. The aim of the present study was to investigate whether NBP is able to attenuate AGE‑induced endothelial dysfunction in vitro, and also elucidate the possible underlying mechanism. An injury model of human umbilical vein endothelial cells (HUVECs) induced by AGEs (200 µg/ml) was established. The results demonstrated that pretreatment with NBP (1‑100 µM) significantly increased HUVEC viability and inhibited the apoptosis induced by AGEs. In addition, AGEs stimulated the expression levels of the receptor for AGEs protein and the downstream protein nuclear factor‑κB in HUVECs, which were inhibited by pretreatment with NBP. Furthermore, it significantly reduced reactive oxygen species generation and the level of the inflammatory cytokines, intercellular cell adhesion molecule‑1 and monocyte chemotactic protein‑1, in HUVECs mediated by AGEs. The current findings indicated that NBP attenuated AGE‑induced endothelial dysfunction by ameliorating inflammation and oxidative stress responses.

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September-2017
Volume 14 Issue 3

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Liu CY, Zhao ZH, Chen ZT, Che CH, Zou ZY, Wu XM, Chen SG, Li YX, Lin HB, Wei XF, Wei XF, et al: DL‑3‑n‑butylphthalide protects endothelial cells against advanced glycation end product‑induced injury by attenuating oxidative stress and inflammation responses. Exp Ther Med 14: 2241-2248, 2017.
APA
Liu, C., Zhao, Z., Chen, Z., Che, C., Zou, Z., Wu, X. ... Huang, H. (2017). DL‑3‑n‑butylphthalide protects endothelial cells against advanced glycation end product‑induced injury by attenuating oxidative stress and inflammation responses. Experimental and Therapeutic Medicine, 14, 2241-2248. https://doi.org/10.3892/etm.2017.4784
MLA
Liu, C., Zhao, Z., Chen, Z., Che, C., Zou, Z., Wu, X., Chen, S., Li, Y., Lin, H., Wei, X., You, J., Huang, H."DL‑3‑n‑butylphthalide protects endothelial cells against advanced glycation end product‑induced injury by attenuating oxidative stress and inflammation responses". Experimental and Therapeutic Medicine 14.3 (2017): 2241-2248.
Chicago
Liu, C., Zhao, Z., Chen, Z., Che, C., Zou, Z., Wu, X., Chen, S., Li, Y., Lin, H., Wei, X., You, J., Huang, H."DL‑3‑n‑butylphthalide protects endothelial cells against advanced glycation end product‑induced injury by attenuating oxidative stress and inflammation responses". Experimental and Therapeutic Medicine 14, no. 3 (2017): 2241-2248. https://doi.org/10.3892/etm.2017.4784