Open Access

Ginsenoside Rb1 inhibits hypoxia-induced epithelial‑mesenchymal transition in ovarian cancer cells by regulating microRNA-25

  • Authors:
    • Dan Liu
    • Ting Liu
    • Yue Teng
    • Wei Chen
    • Le Zhao
    • Xu Li
  • View Affiliations

  • Published online on: August 4, 2017     https://doi.org/10.3892/etm.2017.4889
  • Pages: 2895-2902
  • Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Metastasis frequently occurs in advanced ovarian cancer, which not only leads to substantial mortality but also becomes a major challenge to effective treatment. Epithelial‑mesenchymal transition (EMT) is a key mechanism facilitating cancer metastasis. Targeting the EMT process with more efficacious and less toxic agents to prevent metastasis is of significant therapeutic value for ovarian cancer treatment. The anti‑EMT function and mechanism of ginsenoside Rb1, a monomer composition extracted from the traditional Chinese herb Panax ginseng or P. notoginseng, was investigated in the present study. Western blotting demonstrated that treatment with ginsenoside Rb1 antagonized hypoxia‑induced E‑cadherin downregulation and vimentin upregulation in SKOV3 and 3AO human ovarian cancer cells. Wound healing assays and in vitro migration assays indicated that ginsenoside Rb1 weakened hypoxia‑enhanced cell migration ability. Furthermore, it was demonstrated that microRNA (miR)‑25 is upregulated by hypoxia in ovarian cancer cells, which was attenuated by ginsenoside Rb1 treatment. Additionally, forced expression of miR‑25 in ovarian cancer cells was identified to not only trigger EMT, but also block the suppressive effects of ginsenoside Rb1 on hypoxia‑induced EMT by negatively targeting the E‑cadherin transactivator, EP300. In conclusion, ginsenoside Rb1 may reverse hypoxia‑induced EMT by abrogating the suppression of miR‑25 on EP300 and E‑cadherin, which suggests that ginsenoside Rb1 may be a potential therapeutic candidate for the treatment of ovarian cancer.

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October-2017
Volume 14 Issue 4

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Liu D, Liu T, Teng Y, Chen W, Zhao L and Li X: Ginsenoside Rb1 inhibits hypoxia-induced epithelial‑mesenchymal transition in ovarian cancer cells by regulating microRNA-25. Exp Ther Med 14: 2895-2902, 2017.
APA
Liu, D., Liu, T., Teng, Y., Chen, W., Zhao, L., & Li, X. (2017). Ginsenoside Rb1 inhibits hypoxia-induced epithelial‑mesenchymal transition in ovarian cancer cells by regulating microRNA-25. Experimental and Therapeutic Medicine, 14, 2895-2902. https://doi.org/10.3892/etm.2017.4889
MLA
Liu, D., Liu, T., Teng, Y., Chen, W., Zhao, L., Li, X."Ginsenoside Rb1 inhibits hypoxia-induced epithelial‑mesenchymal transition in ovarian cancer cells by regulating microRNA-25". Experimental and Therapeutic Medicine 14.4 (2017): 2895-2902.
Chicago
Liu, D., Liu, T., Teng, Y., Chen, W., Zhao, L., Li, X."Ginsenoside Rb1 inhibits hypoxia-induced epithelial‑mesenchymal transition in ovarian cancer cells by regulating microRNA-25". Experimental and Therapeutic Medicine 14, no. 4 (2017): 2895-2902. https://doi.org/10.3892/etm.2017.4889