Spontaneously hypertensive rats are sensitive to thoracic aorta damage induced by a hot and humid environment

Xu,Tao; Liu,Fadong; Luo,Yan; Zhu,Lingqin; Niu,Jianguo; Li,Guanghua

doi:10.3892/etm.2017.5050

Spontaneously hypertensive rats are sensitive to thoracic aorta damage induced by a hot and humid environment

Authors:
- Tao Xu
- Fadong Liu
- Yan Luo
- Lingqin Zhu
- Jianguo Niu
- Guanghua Li
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Affiliations: Institute of Basic Medical Science, Ningxia Medical University, Yinchuan, Ningxia 750004, P.R. China, School of Public Health and Management, Ningxia Medical University, Yinchuan, Ningxia 750004, P.R. China, Ningxia Key Laboratory of Cerebrocranial Diseases, Ningxia Medical University, Yinchuan, Ningxia 750004, P.R. China
Published online on: August 28, 2017 https://doi.org/10.3892/etm.2017.5050
Pages: 4383-4390

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Abstract

The purpose of this study was to investigate the effect of a hot and humid environment on thoracic aorta damage in spontaneously hypertensive rats (SHRs). Wistar‑Kyoto (WKY) rats were randomly divided into three groups (n=8 in each group): Control group (WKY‑CN), heat exposure for 8 h group (WKY‑8) and heat exposure for 24 h group (WKY‑24). The CN group was exposed to room temperature (24˚C); WKY‑8 and WKY‑24 group were exposed to heat (32˚C) and 65% relative humidity for 8 and 24 h, respectively. Accordingly, SHRs were randomly divided into three groups (n=8 each group): SHR‑CN, SHR‑8 and SHR‑24. After 7 days of heat exposure, the weight, food consumption and blood pressure of rats was measured. Noradrenaline (NA)‑induced contraction of aorta rings was measured using an organ bath system, and vascular morphology was observed. Expression levels of apoptotic genes and proteins in the thoracic aorta were also measured. The experimental results indicated that, in the heat exposure environment, rat food intake was reduced. Rat weight was significantly increased in all groups except SHR‑24 (all P<0.01 except SHR‑8, P<0.05). Heat exposure significantly increased the blood pressure of rats in the WKY‑24 (P<0.01 for systolic; P<0.05 for diastolic), SHR‑8 and SHR‑24 (all P<0.01) groups. This effect was more notable in SHR compared with WKY. NA‑induced contraction of aorta rings significantly increased in the SHR‑CN group, compared with the WKY‑CN group (P<0.01). Heat exposure significantly elevated the NA‑induced contraction in both 8 h groups compared with the CN groups (P<0.01). This effect was accompanied by structural damage to the thoracic aorta and increased expression of apoptotic genes and proteins. In conclusion, thoracic aorta damages in SHRs were more sensitive to heat exposure. The enhanced NA‑induced contraction may have partly been due to increased apoptosis in the thoracic aorta.

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