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Article

Inhibition of miR‑27a suppresses the inflammatory response via the p38/MAPK pathway in intervertebral disc cells

  • Authors:
    • Zhenguo Cao
    • Liang Chen
  • View Affiliations / Copyright

    Affiliations: Department of Orthopaedic Surgery, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215006, P.R. China
  • Pages: 4572-4578
    |
    Published online on: August 28, 2017
       https://doi.org/10.3892/etm.2017.5053
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Abstract

The current study aimed to investigate the role of miR‑27a in intervertebral disc degeneration (IDD) and to examine the underlying mechanisms. Quantitative polymerase chain reaction (qPCR) was performed to detect the expression level of miR‑27a in the nucleus pulposus (NP) tissues of patients with IDD, and the results revealed an increasing expression of miR‑27a in IDD compared with the control. To further investigate the role of miR‑27a in IDD, a stable human NP cell line with low miR‑27a expression was generated by transfecting cells with a lentiviral antigomiR‑27a inhibitor. In addition, a human NP cell inflammation model was established by lipopolysaccharide (LPS; 10 µM) stimulation. The miR‑27a expression in NP cells was determined by qPCR, while the expression of its target proteins; p‑p38 and nuclear factor (NF‑κB) was measured by western blot analysis. Furthermore, the mRNA and protein expression levels of proinflammatory factors, including interleukin (IL)‑1β, IL‑6 and tumor necrosis factor‑α (TNF‑α), were also evaluated by qPCR and ELISA, respectively. The current results confirmed that miR‑27a was significantly upregulated in IDD. In vitro, downregulation of miR‑27a in LPS‑stimulated NP cells by transfection with the miR‑27a inhibitor resulted in suppression of p‑p38 and NF‑κB expression levels. Furthermore, the production of the proinflammatory factors IL‑1β, IL‑6 and TNF‑α was significantly reduced in LPS‑stimulated NP cells with downregulated miR‑27a. In conclusion, miR‑27a may function as a promoter in IDD development, while inhibition of miR‑27a may suppress proinflammatory factors released by intervertebral disc cells by regulating the p38/mitogen‑activated protein kinase (MAPK) signaling pathway.
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1 

Vos T, Flaxman AD, Naghavi M, Lozano R, Michaud C, Ezzati M, Shibuya K, Salomon JA, Abdalla S, Aboyans V, et al: Years lived with disability (YLDs) for 1160 sequelae of 289 diseases and injuries 1990–2010: A systematic analysis for the Global Burden of Disease Study 2010. Lancet. 380:2163–2196. 2012. View Article : Google Scholar : PubMed/NCBI

2 

Gore M, Sadosky A, Stacey BR, Tai KS and Leslie D: The burden of chronic low back pain: Clinical comorbidities, treatment patterns, and health care costs in usual care settings. Spine (Phila Pa 1976). 37:E668–E677. 2012. View Article : Google Scholar : PubMed/NCBI

3 

Millecamps M, Tajerian M, Naso L, Sage EH and Stone LS: Lumbar intervertebral disc degeneration associated with axial and radiating low back pain in ageing SPARC-null mice. Pain. 153:1167–1179. 2012. View Article : Google Scholar : PubMed/NCBI

4 

Adams MA and Roughley PJ: What is intervertebral disc degeneration, and what causes it? Spine (Phila Pa 1976). 31:2151–2161. 2006. View Article : Google Scholar : PubMed/NCBI

5 

Mayer JE, Iatridis JC, Chan D, Qureshi SA, Gottesman O and Hecht AC: Genetic polymorphisms associated with intervertebral disc degeneration. Spine J. 13:299–317. 2013. View Article : Google Scholar : PubMed/NCBI

6 

Freemont AJ: The cellular pathobiology of the degenerate intervertebral disc and discogenic back pain. Rheumatology (Oxford). 48:5–10. 2009. View Article : Google Scholar : PubMed/NCBI

7 

Li Z, Liang J, Wu WK, Yu X, Yu J, Weng X and Shen J: Leptin activates RhoA/ROCK pathway to induce cytoskeleton remodeling in nucleus pulposus cells. Int J Mol Sci. 15:1176–1188. 2014. View Article : Google Scholar : PubMed/NCBI

8 

Li Z, Shen J, Wu WK, Yu X, Liang J, Qiu G and Liu J: The role of leptin on the organization and expression of cytoskeleton elements in nucleus pulposus cells. J Orthop Res. 31:847–857. 2013. View Article : Google Scholar : PubMed/NCBI

9 

Ellman MB, Kim JS, An HS, Chen D, KC R, An J, Dittakavi T, van Wijnen AJ, Cs-Szabo G, Li X, et al: Toll-like receptor adaptor signaling molecule MyD88 on intervertebral disk homeostasis: In vitro, ex vivo studies. Gene. 505:283–290. 2012. View Article : Google Scholar : PubMed/NCBI

10 

Iwata M, Ochi H, Asou Y, Haro H, Aikawa T, Harada Y, Nezu Y, Yogo T, Tagawa M and Hara Y: Variations in gene and protein expression in canine chondrodystrophic nucleus pulposus cells following long-term three-dimensional culture. PLoS One. 8:e631202013. View Article : Google Scholar : PubMed/NCBI

11 

Lee RC, Feinbaum RL and Ambros V: The C. Elegans heterochronic gene lin-4 encodes small RNAs with antisense complementarity to lin-14. Cell. 75:843–854. 1993. View Article : Google Scholar : PubMed/NCBI

12 

Bartel DP: MicroRNAs: Genomics, biogenesis, mechanism, and function. Cell. 116:281–297. 2004. View Article : Google Scholar : PubMed/NCBI

13 

Teague EM, Print CG and Hull ML: The role of microRNAs in endometriosis and associated reproductive conditions. Hum Reprod Update. 16:142–165. 2010. View Article : Google Scholar : PubMed/NCBI

14 

Croce CM: Causes and consequences of microRNA dysregulation in cancer. Nat Rev Genet. 10:704–714. 2009. View Article : Google Scholar : PubMed/NCBI

15 

Wang HQ, Yu XD, Liu ZH, Cheng X, Samartzis D, Jia LT, Wu SX, Huang J, Chen J and Luo ZJ: Deregulated miR-155 promotes Fas-mediated apoptosis in human intervertebral disc degeneration by targeting FADD and caspase-3. J Pathol. 225:232–242. 2011. View Article : Google Scholar : PubMed/NCBI

16 

Yu X, Li Z, Shen J, Wu WK, Liang J, Weng X and Qiu G: MicroRNA-10b promotes nucleus pulposus cell proliferation through RhoC-Akt pathway by targeting HOXD10 in intervetebral disc degeneration. PLoS One. 8:e830802013. View Article : Google Scholar : PubMed/NCBI

17 

Ohrt-Nissen S, Dөssing KB, Rossing M, Lajer C, Vikeså J, Nielsen FC, Friis-Hansen L and Dahl B: Characterization of miRNA expression in human degenerative lumbar disks. Connect Tissue Res. 54:197–203. 2013. View Article : Google Scholar : PubMed/NCBI

18 

Liu H, Huang X, Liu X, Xiao S, Zhang Y, Xiang T, Shen X, Wang G and Sheng B: miR-21 promotes human nucleus pulposus cell proliferation through PTEN/AKT signaling. Int J Mol Sci. 15:4007–4018. 2014. View Article : Google Scholar : PubMed/NCBI

19 

Liu G, Cao P, Chen H, Yuan W, Wang J and Tang X: MiR-27a regulates apoptosis in nucleus pulposus cells by targeting PI3K. PLoS One. 8:e752512013. View Article : Google Scholar : PubMed/NCBI

20 

Ji J, Zhang J, Huang G, Qian J, Wang X and Mei S: Over-expressed microRNA-27a and 27b influence fat accumulation and cell proliferation during rat hepatic stellate cell activation. FEBS Lett. 583:759–766. 2009. View Article : Google Scholar : PubMed/NCBI

21 

Liu T, Tang H, Lang Y, Liu M and Li X: MicroRNA-27a functions as an oncogene in gastric adenocarcinoma by targeting prohibitin. Cancer Lett. 273:233–242. 2009. View Article : Google Scholar : PubMed/NCBI

22 

Ma Y, Yu S, Zhao W, Lu Z and Chen J: miR-27a regulates the growth, colony formation and migration of pancreatic cancer cells by targeting Sprouty2. Cancer Lett. 298:150–158. 2010. View Article : Google Scholar : PubMed/NCBI

23 

Zhao B, Yu Q, Li H, Guo X and He X: Characterization of microRNA expression profiles in patients with intervertebral disc degeneration. Int J Mol Med. 33:43–50. 2014. View Article : Google Scholar : PubMed/NCBI

24 

Pfirrmann CW, Metzdorf A, Zanetti M, Hodler J and Boos N: Magnetic resonance classification of lumbar intervertebral disc degeneration. Spine (Phila Pa 1976). 26:1873–1878. 2001. View Article : Google Scholar : PubMed/NCBI

25 

Zhao L, Lu X and Cao Y: MicroRNA and signal transduction pathways in tumor radiation response. Cell Signal. 25:1625–1634. 2013. View Article : Google Scholar : PubMed/NCBI

26 

Ell B and Kang Y: MicroRNAs as regulators of bone homeostasis and bone metastasis. Bonekey Rep. 3:5492014. View Article : Google Scholar : PubMed/NCBI

27 

Zhao X, Yang L and Hu J: Down-regulation of miR-27a might inhibit proliferation and drug resistance of gastric cancer cells. J Exp Clin Cancer Res. 30:552011. View Article : Google Scholar : PubMed/NCBI

28 

Drayton RM, Dudziec E, Peter S, Bertz S, Hartmann A, Bryant HE and Catto JW: Reduced expression of miRNA-27a modulates cisplatin resistance in bladder cancer by targeting the cystine/glutamate exchanger SLC7A11. Clin Cancer Res. 20:1990–2000. 2014. View Article : Google Scholar : PubMed/NCBI

29 

Hezova R, Kovarikova A, Bienertova-Vasku J, Sachlova M, Redova M, Vasku A, Svoboda M, Radova L, Kiss I, Vyzula R and Slaby O: Evaluation of SNPs in miR-196-a2, miR-27a and miR-146a as risk factors of colorectal cancer. World J Gastroenterol. 18:2827–2831. 2012. View Article : Google Scholar : PubMed/NCBI

30 

Gruber HE, Norton HJ, Sun Y and Hanley EN Jr: Crystal deposits in the human intervertebral disc: Implications for disc degeneration. Spine J. 7:444–450. 2007. View Article : Google Scholar : PubMed/NCBI

31 

Wuertz K, Vo N, Kletsas D and Boos N: Inflammatory and catabolic signalling in intervertebral discs: The roles of NF-kB and MAP kinases. Eur Cell Mater. 23:103–120. 2012.PubMed/NCBI

32 

Kokubo Y, Uchida K, Kobayashi S, Yayama T, Sato R, Nakajima H, Takamura T, Mwaka E, Orwotho N, Bangirana A and Baba H: Herniated and spondylotic intervertebral discs of the human cervical spine: Histological and immunohistological findings in 500 en bloc surgical samples. Laboratory investigation. J Neurosurg Spine. 9:285–295. 2008. View Article : Google Scholar : PubMed/NCBI

33 

Shamji MF, Setton LA, Jarvis W, So S, Chen J, Jing L, Bullock R, Isaacs RE, Brown C and Richardson WJ: Proinflammatory cytokine expression profile in degenerated and herniated human intervertebral disc tissues. Arthritis Rheum. 62:1974–1982. 2010.PubMed/NCBI

34 

Liu C, Yang H, Gao F, Li X, An Y, Wang J and Jin A: Resistin promotes intervertebral disc degeneration by upregulation of ADAMTS-5 through p38MAPK signaling pathway. Spine (Phila Pa 1976). 41:1414–1420. 2016. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Cao Z and Chen L: Inhibition of miR‑27a suppresses the inflammatory response via the p38/MAPK pathway in intervertebral disc cells. Exp Ther Med 14: 4572-4578, 2017.
APA
Cao, Z., & Chen, L. (2017). Inhibition of miR‑27a suppresses the inflammatory response via the p38/MAPK pathway in intervertebral disc cells. Experimental and Therapeutic Medicine, 14, 4572-4578. https://doi.org/10.3892/etm.2017.5053
MLA
Cao, Z., Chen, L."Inhibition of miR‑27a suppresses the inflammatory response via the p38/MAPK pathway in intervertebral disc cells". Experimental and Therapeutic Medicine 14.5 (2017): 4572-4578.
Chicago
Cao, Z., Chen, L."Inhibition of miR‑27a suppresses the inflammatory response via the p38/MAPK pathway in intervertebral disc cells". Experimental and Therapeutic Medicine 14, no. 5 (2017): 4572-4578. https://doi.org/10.3892/etm.2017.5053
Copy and paste a formatted citation
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Spandidos Publications style
Cao Z and Chen L: Inhibition of miR‑27a suppresses the inflammatory response via the p38/MAPK pathway in intervertebral disc cells. Exp Ther Med 14: 4572-4578, 2017.
APA
Cao, Z., & Chen, L. (2017). Inhibition of miR‑27a suppresses the inflammatory response via the p38/MAPK pathway in intervertebral disc cells. Experimental and Therapeutic Medicine, 14, 4572-4578. https://doi.org/10.3892/etm.2017.5053
MLA
Cao, Z., Chen, L."Inhibition of miR‑27a suppresses the inflammatory response via the p38/MAPK pathway in intervertebral disc cells". Experimental and Therapeutic Medicine 14.5 (2017): 4572-4578.
Chicago
Cao, Z., Chen, L."Inhibition of miR‑27a suppresses the inflammatory response via the p38/MAPK pathway in intervertebral disc cells". Experimental and Therapeutic Medicine 14, no. 5 (2017): 4572-4578. https://doi.org/10.3892/etm.2017.5053
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