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Renal sympathetic denervation improves myocardial apoptosis in rats with isoproterenol‑induced heart failure by downregulation of tumor necrosis factor‑α and nuclear factor‑κB

  • Authors:
    • Wei Yao
    • Neng Wang
    • Jin Qian
    • Lu Bai
    • Xiaoxin Zheng
    • Guo Hou
    • Xuan Qiu
    • Bo Yang
  • View Affiliations / Copyright

    Affiliations: Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, P.R. China, Department of Cardiology, Suizhou Hospital, Hubei University of Medicine, Suizhou, Hubei 441300, P.R. China
    Copyright: © Yao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 4104-4110
    |
    Published online on: August 30, 2017
       https://doi.org/10.3892/etm.2017.5066
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Abstract

Chronic congestive heart failure (CHF) is the end outcome of organic heart diseases and one of the major diseases harmful to human health. Renal sympathetic denervation (RSD) is the anatomical basis of transcatheter renal sympathetic nerve ablation within the renal artery. To date, the roles of norepinephrine and angiotensin II (Ang II) in myocardial apoptosis and their underlying mechanisms have not been well explored. The aim of the present study was to verify the hypothesis that RSD is likely to inhibit myocardial apoptosis by inhibiting the release of norepinephrine and Ang II. An isoproterenol‑induced CHF rat model was established, and the effects of RSD on myocardial apoptosis were examined using flow cytometry and TUNEL staining. The expression of factors associated with myocardial apoptosis, including p53, tumor necrosis factor‑α (TNF‑α), nuclear factor‑κB (NF‑κB), caspase-2 and -3, were measured using quantitative polymerase chain reaction and western blot analysis. The results indicated that the mRNA levels of p53, TNF‑α, NF‑κB, caspase‑2 and ‑3 were significantly reduced in the myocardial tissues of rats in the CHF+RSD group when compared with the levels in the CHF+sham group (P<0.01 for all). In addition, the protein levels of p53, TNF‑α, NF‑κB and caspases-2 and -3 were decreased by 42.6, 41.3, 46.7, 30.0 and 35.8%, respectively, in myocardial tissues of rats in the CHF+RSD group in comparison with the CHF+sham group (P<0.01 for all). Furthermore, myocardial apoptosis was improved in rats in the CHF+RSD group compared with that in the CHF+sham group (P<0.01). In conclusion, the present study provides a theoretical basis for application of RSD in the treatment of CHF.
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Copy and paste a formatted citation
Spandidos Publications style
Yao W, Wang N, Qian J, Bai L, Zheng X, Hou G, Qiu X and Yang B: Renal sympathetic denervation improves myocardial apoptosis in rats with isoproterenol‑induced heart failure by downregulation of tumor necrosis factor‑α and nuclear factor‑κB. Exp Ther Med 14: 4104-4110, 2017.
APA
Yao, W., Wang, N., Qian, J., Bai, L., Zheng, X., Hou, G. ... Yang, B. (2017). Renal sympathetic denervation improves myocardial apoptosis in rats with isoproterenol‑induced heart failure by downregulation of tumor necrosis factor‑α and nuclear factor‑κB. Experimental and Therapeutic Medicine, 14, 4104-4110. https://doi.org/10.3892/etm.2017.5066
MLA
Yao, W., Wang, N., Qian, J., Bai, L., Zheng, X., Hou, G., Qiu, X., Yang, B."Renal sympathetic denervation improves myocardial apoptosis in rats with isoproterenol‑induced heart failure by downregulation of tumor necrosis factor‑α and nuclear factor‑κB". Experimental and Therapeutic Medicine 14.5 (2017): 4104-4110.
Chicago
Yao, W., Wang, N., Qian, J., Bai, L., Zheng, X., Hou, G., Qiu, X., Yang, B."Renal sympathetic denervation improves myocardial apoptosis in rats with isoproterenol‑induced heart failure by downregulation of tumor necrosis factor‑α and nuclear factor‑κB". Experimental and Therapeutic Medicine 14, no. 5 (2017): 4104-4110. https://doi.org/10.3892/etm.2017.5066
Copy and paste a formatted citation
x
Spandidos Publications style
Yao W, Wang N, Qian J, Bai L, Zheng X, Hou G, Qiu X and Yang B: Renal sympathetic denervation improves myocardial apoptosis in rats with isoproterenol‑induced heart failure by downregulation of tumor necrosis factor‑α and nuclear factor‑κB. Exp Ther Med 14: 4104-4110, 2017.
APA
Yao, W., Wang, N., Qian, J., Bai, L., Zheng, X., Hou, G. ... Yang, B. (2017). Renal sympathetic denervation improves myocardial apoptosis in rats with isoproterenol‑induced heart failure by downregulation of tumor necrosis factor‑α and nuclear factor‑κB. Experimental and Therapeutic Medicine, 14, 4104-4110. https://doi.org/10.3892/etm.2017.5066
MLA
Yao, W., Wang, N., Qian, J., Bai, L., Zheng, X., Hou, G., Qiu, X., Yang, B."Renal sympathetic denervation improves myocardial apoptosis in rats with isoproterenol‑induced heart failure by downregulation of tumor necrosis factor‑α and nuclear factor‑κB". Experimental and Therapeutic Medicine 14.5 (2017): 4104-4110.
Chicago
Yao, W., Wang, N., Qian, J., Bai, L., Zheng, X., Hou, G., Qiu, X., Yang, B."Renal sympathetic denervation improves myocardial apoptosis in rats with isoproterenol‑induced heart failure by downregulation of tumor necrosis factor‑α and nuclear factor‑κB". Experimental and Therapeutic Medicine 14, no. 5 (2017): 4104-4110. https://doi.org/10.3892/etm.2017.5066
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