Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
November-2017 Volume 14 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
November-2017 Volume 14 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Mast cell chymase promotes hypertrophic scar fibroblast proliferation and collagen synthesis by activating TGF‑β1/Smads signaling pathway

  • Authors:
    • Hongming Chen
    • Yanwen Xu
    • Guanbin Yang
    • Qianqian Zhang
    • Xun Huang
    • Liming Yu
    • Xianglin Dong
  • View Affiliations / Copyright

    Affiliations: Department of Burns and Plastic Surgery, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang Uyghur Autonomous Region 830011, P.R. China
  • Pages: 4438-4442
    |
    Published online on: August 31, 2017
       https://doi.org/10.3892/etm.2017.5082
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

The present study assessed the existence of mast cell chymase in hypertrophic scars and determined whether chymase promotes fibrosis via the transforming growth factor (TGF)‑β1/Smads signaling pathway. Five patients with hypertrophic scars and another five patients subjected to repair and reconstruction of other tissue defects were included in the present study. To detect the existence of mast cells and mast cell chymase in hypertrophic scars, immunohistochemistry was employed. To test the effect of chymase on TGF‑β1, angiotensin, and type I and III collagen mRNA expression in isolated hypertrophic scar fibroblasts in vitro, reverse‑transcription quantitative PCR was performed. To investigate how chymase affects TGF‑β1, phosphorylated (P)‑Smad2/3 as well as Smad4 and Smad7 protein expression, western blot analysis was used. Mast cell chymase was identified to promote the mRNA expression of TGF‑β1, angiotensin, and type I and III collagen in hypertrophic scar fibroblasts in a time‑ and dose‑dependent manner. Furthermore, treatment with 60 ng/ml mast cell chymase for 12 h led to the upregulation of TGF‑β1, P‑Smad2/3, Smad4 and Smad7 in hypertrophic scar fibroblasts. The present study demonstrated that mast cells and chymase are present in hypertrophic scars, and chymase promotes hypertrophic scar fibroblast proliferation and collagen synthesis by activating the TGF‑β1/Smads signaling pathway.
View Figures

Figure 1

Figure 2

Figure 3

View References

1 

Beldon P: Abnormal scar formation in wound healing. Nurs Times. 96:44–45. 2000.PubMed/NCBI

2 

Shi Y and Massagé J: Mechanisms of TGF-beta signaling from cell membrance to the nucleus. Cell. 113:685–700. 2003. View Article : Google Scholar : PubMed/NCBI

3 

Douaiher J, Succar J, Lancerotto L, Gurish MF, Orgill DP, Hamilton MJ, Krilis SA and Stevens RL: Development of mast cells and importance of their tryptase and chymase serine proteases in inflammation and wound healing. Adv Immunol. 122:211–252. 2014. View Article : Google Scholar : PubMed/NCBI

4 

Zhao XY, Zhao LY, Zheng QS, Su JL, Guan H, Shang FJ, Niu XL, He YP and Lu XL: Chymase induces profibrotic response via transforming growth factor-beta 1/Smad activation in rat cardiac fibroblasts. Mol Cell Biochem. 310:159–166. 2008. View Article : Google Scholar : PubMed/NCBI

5 

Lindstedt KA, Wang Y, Shiota N, Saarinen J, Hyytiäinen M, Kokkonen JO, Keski-Oja J and Kovanen PT: Activation of paracrine TGF-beta1 signaling upon stimulation and degranulation of rat serosal mast cells: A novel function for chymase. FASEB J. 15:1377–1388. 2001. View Article : Google Scholar : PubMed/NCBI

6 

Muto T and Fukami H: Recent chymase inhibitors and their effects in in vivo models. IDrugs. 5:1141–1150. 2002.PubMed/NCBI

7 

Taipale J, Lohi J, Saarinen J, Kovanen PT and Keski-Oja J: Human mast cell chymase and leukocyte elastase release latent transforming growth factor-beta 1 from the extracellular matrix of cultured human epithelial and endothelial cells. J Biol Chem. 270:4689–4696. 1995. View Article : Google Scholar : PubMed/NCBI

8 

Algermissen B, Hermes B, Feldmann-Boeddeker I, Bauer F and Henz BM: Mast cell chymase and tryptase during tissue turnover: Analysis on in vitro mitogenesis of fibroblasts and keratinocytes and alterations in cutaneous scars. Exp Dermatol. 8:193–198. 1999. View Article : Google Scholar : PubMed/NCBI

9 

Fan SQ, Cai JL, Qin LY, Wang ZH, Liu ZZ and Sun ML: Effect of heparin on production of transforming growth factor (TGF)-beta1 and TGF-beta1 mRNA expression by human normal skin and hyperplastic scar fibroblasts. Ann Plast Surg. 60:299–305. 2008. View Article : Google Scholar : PubMed/NCBI

10 

Cowin AJ, Holmes TM, Brosnan P and Ferguson MW: Expression of TGF-beta and its receptors in murine fetal and adult dermal wounds. Eur J Dermatol. 11:424–431. 2001.PubMed/NCBI

11 

Walraven M, Gouverneur M, Middelkoop E, Beelen RH and Ulrich MM: Altered TGF-β signaling in fetal fibroblasts: What is known about the underlying mechanisms? Wound Repair Regen. 22:3–13. 2014. View Article : Google Scholar : PubMed/NCBI

12 

Blobe GC, Schiemann WP and Lodish HF: Role of transforming growth factor beta in human disease. N Engl J Med. 342:1350–1358. 2000. View Article : Google Scholar : PubMed/NCBI

13 

Yamamoto T, Hartmann K, Eckes B and Krieg T: Role of stem cell factor and monocyte chemoattrctant protein in the interaction between fibroblasts and mast cells in fibrosis. J Dermatol Sci. 26:106–111. 2001. View Article : Google Scholar : PubMed/NCBI

14 

Yu H, Bock O, Bayat A, Ferguson MW and Mrowietz U: Decreased expression of inhibitory SMAD6 and SMAD7 in keloid scarring. J Plast Reconstr Aesthet Surg. 59:221–229. 2006. View Article : Google Scholar : PubMed/NCBI

15 

Singer AJ and Clark RA: Cutaneous wound healing. N Engl J Med. 341:738–746. 1999. View Article : Google Scholar : PubMed/NCBI

16 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

17 

Hermes B, Feldmann-Böddeker I, Welker P, Algermissen B, Steckelings MU, Grabbe J and Henz BM: Altered expression of mast cell chymase and tryptase and of c-Kit in human cutaneous scar tissue. J Invest Dermatol. 114:51–55. 2000. View Article : Google Scholar : PubMed/NCBI

18 

Miller HR, Wright SH, Knight PA and Thornton EM: A novel function for transforming growth factor-beta1: Upregulation of the expression and the IgE-independent extracellular release of a mucosal mast cell granule-specific beta-chymase, mouse mast cell protease-1. Blood. 93:3473–3486. 1999.PubMed/NCBI

19 

Moyer KE, Saggers GC and Ehrlich HP: Mast cells promote fibroblast populated collagen lattice contraction through gap junction intercellular communication. Wound Repair Regen. 12:269–275. 2004. View Article : Google Scholar : PubMed/NCBI

20 

Castagnoli C, Stella M, Berthod C, Magliacani G and Richiardi PM: TNF production and hypertrophic scarring. Cell Immunol. 147:51–63. 1993. View Article : Google Scholar : PubMed/NCBI

21 

Sakaguchi H, Takai S, Sakaguchi M, Sugiyama T, Ishihara T, Yao Y, Miyazaki M and Ikeda T: Chymase and angiotensin converting enzyme activities in a hamster model of glaucoma filtering surgery. Curr Eye Res. 24:325–331. 2002. View Article : Google Scholar : PubMed/NCBI

22 

Younan G, Suber F, Xing W, Shi T, Kunori Y, Abrink M, Pejler G, Schlenner SM, Rodewald HR, Moore FD Jr, et al: The inflammatory response following an epidermal burn depends on the activities of mouse mast cell proteases 4 and 5. J Immunol. 185:7681–7690. 2010. View Article : Google Scholar : PubMed/NCBI

23 

Fan JM, Ng YY, Hill PA, Nikolic-Paterson DJ, Mu W, Atkins RC and Lan HY: Transforming growth factor-beta regulates tubular epithelial-myofibroblast transdiffetrntiation in vitro. Kideny Int. 56:1455–1467. 1999. View Article : Google Scholar

24 

Willis BC and Borok Z: TGF-beta-induced EMT: Mechanisms and implications for fibrotic lung disease. Am J Physiol Lung Cell Mol Physiol. 293:L525–L534. 2007. View Article : Google Scholar : PubMed/NCBI

25 

Ghahary A, Shen YJ, Scott PG, Gong Y and Tredget EE: Enhanced expression of mRNA for transforming growth factor-beta, type I and type III procollagen in human postburn hypertrophic scar tissues. J Lab Clin Med. 122:465–473. 1993.PubMed/NCBI

26 

Tredget EE, Wang R, Shen Q, Scott PG and Ghahary A: Transforming growth factor-beta mRNA and protein in hypertrophic scar tissues and fibroblasts: Antagonism by IFN-alpha and IFN-gamma in vitro and in vivo. J Interferon Cytokine Res. 20:143–151. 2000. View Article : Google Scholar : PubMed/NCBI

27 

Verhaegen PD, van Zuijlen PP, Pennings NM, van Marle J, Niessen FB, van der Horst CM and Middelkoop E: Differences in collagen architecture between keloid, hypertrophic scar, normotrophic scar, and normal skin: An objective histopathological analysis. Wound Repair Regen. 17:649–656. 2009. View Article : Google Scholar : PubMed/NCBI

28 

Miyazono K, Maeda S and Imamura T: BMP receptor signaling: Transcriptional targets, regulation of signals, and signaling cross-talk. Cytokine Growth Factor Rev. 16:251–263. 2005. View Article : Google Scholar : PubMed/NCBI

29 

Tao SJ and Sampath K: Alternative splicing of SMADs in differentiation and tissue homeostasis. Dev Growth Differ. 52:335–342. 2010. View Article : Google Scholar : PubMed/NCBI

30 

Dabiri G, Campaner A, Morgan JR and Van De Water L: A TGF-beta1-dependent autocrine loop regulates the structure of focal adhesions in hypertrophic scar fibroblasts. J Invest Dermatol. 126:963–970. 2006. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Chen H, Xu Y, Yang G, Zhang Q, Huang X, Yu L and Dong X: Mast cell chymase promotes hypertrophic scar fibroblast proliferation and collagen synthesis by activating TGF‑β1/Smads signaling pathway. Exp Ther Med 14: 4438-4442, 2017.
APA
Chen, H., Xu, Y., Yang, G., Zhang, Q., Huang, X., Yu, L., & Dong, X. (2017). Mast cell chymase promotes hypertrophic scar fibroblast proliferation and collagen synthesis by activating TGF‑β1/Smads signaling pathway. Experimental and Therapeutic Medicine, 14, 4438-4442. https://doi.org/10.3892/etm.2017.5082
MLA
Chen, H., Xu, Y., Yang, G., Zhang, Q., Huang, X., Yu, L., Dong, X."Mast cell chymase promotes hypertrophic scar fibroblast proliferation and collagen synthesis by activating TGF‑β1/Smads signaling pathway". Experimental and Therapeutic Medicine 14.5 (2017): 4438-4442.
Chicago
Chen, H., Xu, Y., Yang, G., Zhang, Q., Huang, X., Yu, L., Dong, X."Mast cell chymase promotes hypertrophic scar fibroblast proliferation and collagen synthesis by activating TGF‑β1/Smads signaling pathway". Experimental and Therapeutic Medicine 14, no. 5 (2017): 4438-4442. https://doi.org/10.3892/etm.2017.5082
Copy and paste a formatted citation
x
Spandidos Publications style
Chen H, Xu Y, Yang G, Zhang Q, Huang X, Yu L and Dong X: Mast cell chymase promotes hypertrophic scar fibroblast proliferation and collagen synthesis by activating TGF‑β1/Smads signaling pathway. Exp Ther Med 14: 4438-4442, 2017.
APA
Chen, H., Xu, Y., Yang, G., Zhang, Q., Huang, X., Yu, L., & Dong, X. (2017). Mast cell chymase promotes hypertrophic scar fibroblast proliferation and collagen synthesis by activating TGF‑β1/Smads signaling pathway. Experimental and Therapeutic Medicine, 14, 4438-4442. https://doi.org/10.3892/etm.2017.5082
MLA
Chen, H., Xu, Y., Yang, G., Zhang, Q., Huang, X., Yu, L., Dong, X."Mast cell chymase promotes hypertrophic scar fibroblast proliferation and collagen synthesis by activating TGF‑β1/Smads signaling pathway". Experimental and Therapeutic Medicine 14.5 (2017): 4438-4442.
Chicago
Chen, H., Xu, Y., Yang, G., Zhang, Q., Huang, X., Yu, L., Dong, X."Mast cell chymase promotes hypertrophic scar fibroblast proliferation and collagen synthesis by activating TGF‑β1/Smads signaling pathway". Experimental and Therapeutic Medicine 14, no. 5 (2017): 4438-4442. https://doi.org/10.3892/etm.2017.5082
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team