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Abnormal expression of microRNA‑575 leads to missed abortion through regulating apoptosis and angiogenesis

  • Authors:
    • Shuqin Xia
    • Yihui Zhen
    • Hongsheng Ma
    • Aiming Wang
  • View Affiliations / Copyright

    Affiliations: Department of Gynecology and Obstetrics, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China, Department of Gynecology and Obstetrics, Navy General Hospital PLA China, Beijing 100048, P.R. China
    Copyright: © Xia et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3993-4000
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    Published online on: August 31, 2017
       https://doi.org/10.3892/etm.2017.5086
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Abstract

Numerous microRNA (miR) are important for placental development and function. miR‑575 has been demonstrated to be upregulated in maternal placenta in patients who have experienced a miscarriage. The present study aimed to explore the role of abnormal expression of miR‑575 in missed abortion (MA) and to further analyze the potential molecular mechanisms. Embryo villus tissue samples were extracted from 10 childless women with MA and 10 fertile women without a history of MA. Additionally, human choriocarcinoma cells, JEG‑3, were used in the present study, which were transfected with miR‑575 mimic, inhibitor and scramble. The expression of miR‑575 in embryo villus tissues and in JEG‑3 cells was detected by reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR). Apoptosis in villus tissues of patients with MA and in JEG‑3 cells of miR‑575 mimic, inhibitor and scramble groups were detected by flow cytometry. Furthermore, the expression levels of apoptosis‑related proteins, including B‑cell lymphoma 2 (Bcl‑2), Bcl‑2‑associated X protein (Bax) and phosphorylated (p)‑p53, and angiogenesis‑related proteins, including vascular endothelial growth factor (VEGF) and angiopoietin 2 (Ang‑2), were measured by RT‑qPCR and western blotting. Additionally, the target of miR‑575 was predicted and clarified by luciferase reporter assay. miR‑575 was significantly overexpressed in MA villus tissue compared with normal tissue (P<0.05). The percentage of apoptotic cells in MA embryo villus tissue was significantly higher than that in normal tissue (P<0.05). After JEG‑3 cells were transfected with miR‑575 inhibitor, the expression of miR‑575 and the percentage of apoptotic cells decreased significantly compared with the control (P<0.05). MiR‑575 suppression significantly increased the expression of Bcl‑2 (P<0.05), and decreased the expressions of Bax (P<0.05) and p‑p53 (P<0.01) compared with the control. Furthermore, miR‑575 suppression significantly increased the expressions of angiogenesis‑related proteins, Ang‑2 and VEGF (P<0.01). Superoxide dismutase 2 was identified as the target of miR‑575. Therefore, abnormal expression of miR‑575 may lead to MA through regulating apoptosis and angiogenesis. Inhibition of miR‑575 may inhibit apoptosis and promote angiogenesis in MA.
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1 

Cao W, Wenlin XU, Chen T, Wang X, Wang X, Qiu J, Chen N and Mao Y: CD4+CD25+FoxP3+ regulatory T cells and cytokines interact with estradiol in cases of missed abortion. Exp Ther Med. 7:417–422. 2014. View Article : Google Scholar : PubMed/NCBI

2 

Sebire NJ, Thornton S, Hughes K, Snijders RJ and Nicolaides KH: The prevalence and consequences of missed abortion in twin pregnancies at 10 to 14 weeks of gestation. Br J Obstet Gynaecol. 104:847–848. 1997. View Article : Google Scholar : PubMed/NCBI

3 

Zhang X, Li J, Gu Y, Zhao Y, Wang Z and Jia G: A pilot study on environmental and behavioral factors related to missed abortion. Environ Health Prev Med. 16:273–278. 2011. View Article : Google Scholar : PubMed/NCBI

4 

O'Connell RM, Rao DS, Chaudhuri AA and Baltimore D: Physiological and pathological roles for microRNAs in the immune system. Nat Rev Immunol. 10:111–122. 2010. View Article : Google Scholar : PubMed/NCBI

5 

Longtine MS, Chen B, Odibo AO, Zhong Y and Nelson DM: Villous trophoblast apoptosis is elevated and restricted to cytotrophoblasts in pregnancies complicated by preeclampsia, IUGR, or preeclampsia with IUGR. Placenta. 5:352–359. 2012. View Article : Google Scholar

6 

Chatzaki E, Makrigiannakis A, Margioris AN, Kouimtzoglou E and Gravanis A: The Fas/FasL apoptotic pathway is involved in kappa-opioid-induced apoptosis of human endometrial stromal cells. Mol Hum Reprod. 7:867–874. 2001. View Article : Google Scholar : PubMed/NCBI

7 

Jerzak M and Bischof P: Apoptosis in the first trimester human placenta: The role in maintaining immune privilege at the maternal-foetal interface and in the trophoblast remodelling. Eur J Obstet Gynecol Reprod Biol. 100:138–142. 2002. View Article : Google Scholar : PubMed/NCBI

8 

Halperin R, Peller S, Rotschild M, Bukovsky I and Schneider D: Placental apoptosis in normal and abnormal pregnancies. Gynecol Obstet Invest. 50:84–87. 2000. View Article : Google Scholar : PubMed/NCBI

9 

Chen H, Deng X, Yang Y, Shen Y, Chao L, Wen Y and Sun Y: Expression of GRIM-19 in missed abortion and possible pathogenesis. Fertil Steril. 103:138–146. 2015. View Article : Google Scholar : PubMed/NCBI

10 

Nelissen EC, van Montfoort AP, Dumoulin JC and Evers JL: Epigenetics and the placenta. Hum Reprod Update. 17:397–417. 2011. View Article : Google Scholar : PubMed/NCBI

11 

Miura K, Miura S, Yamasaki K, Higashijima A, Kinoshita A, Yoshiura K and Masuzaki H: Identification of pregnancy-associated microRNAs in maternal plasma. Clin Chem. 56:1767–1771. 2010. View Article : Google Scholar : PubMed/NCBI

12 

Hosseini MK, Gunel T and Gumusoglu E: MicroRNA profiling in miscarriage patients. IJMM. 34:S94. 2014.

13 

Ward RH, Modell B, Petrou M, Karagözlu F and Douratsos E: Method of sampling chorionic villi in first trimester of pregnancy under guidance of real time ultrasound. Br Med J (Clin Res Ed). 286:1542–1544. 1983. View Article : Google Scholar : PubMed/NCBI

14 

Livak K and Schmittgen TD: Analysis of relative gene expression data using real-tie quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

15 

Zhu LJ, Chen YP, Chen BJ and Mei XH: Changes in reactive oxygen species, superoxide dismutase and hypoxia-inducible factor-1α levels in missed abortion. Int J Clin Exp Med. 7:2179–2184. 2014.PubMed/NCBI

16 

Savion S, Lepsky E, Orenstein H, Carp H, Shepshelovich J, Torchinsky A, Fein A and Toder V: Apoptosis in the uterus of mice with pregnancy loss. Am J Reprod Immunol. 47:118–127. 2002. View Article : Google Scholar : PubMed/NCBI

17 

Choi HK, Choi BC, Lee SH, Kim JW, Cha KY and Baek KH: Expression of angiogenesis- and apoptosis-related genes in chorionic villi derived from recurrent pregnancy loss patients. Mol Reprod Dev. 66:24–31. 2003. View Article : Google Scholar : PubMed/NCBI

18 

Cinar O, Kara F and Can A: Potential role of decidual apoptosis in the pathogenesis of miscarriages. Gynecol Endocrinol. 28:382–385. 2012. View Article : Google Scholar : PubMed/NCBI

19 

Nair RR, Khanna A and Singh K: Association of FAS-1377 G>A and FAS-670 A>G functional polymorphisms of FAS gene of cell death pathway with recurrent early pregnancy loss risk. J Reprod Immunol. 93:114–118. 2012. View Article : Google Scholar : PubMed/NCBI

20 

Levy R and Nelson D: CURRENT TOPIC: To be, or not to be, that is the question. Apoptosis In human trophoblast. Placenta. 21:1–13. 2000. View Article : Google Scholar : PubMed/NCBI

21 

Kaare M, Bützow R, Ulander VM, Kaaja R, Aittomäki K and Painter JN: Study of p53 gene mutations and placental expression in recurrent miscarriage cases. Reprod Biomed Online. 18:430–435. 2009. View Article : Google Scholar : PubMed/NCBI

22 

Chen Y, Shen D, Gu Y, Zhong P, Xie J and Song Q: The diagnostic value of Ki-67, P53 and P63 in distinguishing partial Hydatidiform mole from hydropic abortion. Wien Klin Wochenschr. 124:184–187. 2012. View Article : Google Scholar : PubMed/NCBI

23 

Taylor DD and Gercel-Taylor C: Alterations in T-cell signal transduction molecules associated with recurrent spontaneous pregnancy loss. J Reprod Immunol. 63:137–154. 2004. View Article : Google Scholar : PubMed/NCBI

24 

Rajakumar A and Conrad KP: Expression, ontogeny, and regulation of hypoxia-inducible transcription factors in the human placenta. Biol Reprod. 63:559–569. 2000. View Article : Google Scholar : PubMed/NCBI

25 

Lim KH, Zhou Y, Janatpour M, McMaster M, Bass K, Chun SH and Fisher SJ: Human cytotrophoblast differentiation/invasion is abnormal in pre-eclampsia. Am J Pathol. 151:1809–1818. 1997.PubMed/NCBI

26 

Fang Y, Yu S, Ma Y, Sun P, Ma D, Ji C and Kong B: Association of Dll4/notch and HIF-1a-VEGF signaling in the angiogenesis of missed abortion. PLoS One. 8:e706672013. View Article : Google Scholar : PubMed/NCBI

27 

Arjamaa O, Nikinmaa M, Salminen A and Kaarniranta K: Regulatory role of HIF-1alpha in the pathogenesis of age-related macular degeneration (AMD). Ageing Res Rev. 8:349–358. 2009. View Article : Google Scholar : PubMed/NCBI

28 

Çöl-Madendag I, Madendag Y, Altinkaya SÖ, Bayramoglu H and Danisman N: The role of VEGF and its receptors in the etiology of early pregnancy loss. Gynecol Endocrinol. 30:153–156. 2014. View Article : Google Scholar : PubMed/NCBI

29 

Folkman J and Klagsbrun M: Angiogenic factors. Science. 235:442–447. 1987. View Article : Google Scholar : PubMed/NCBI

30 

Maisonpierre PC, Suri C, Jones PF, Bartunkova S, Wiegand SJ, Radziejewski C, Compton D, McClain J, Aldrich TH, Papadopoulos N, et al: Angiopoietin-2, a natural antagonist for Tie2 that disrupts in vivo angiogenesis. Science. 277:55–60. 1997. View Article : Google Scholar : PubMed/NCBI

31 

Valenzuela DM, Griffiths JA, Rojas J, Aldrich TH, Jones PF, Zhou H, McClain J, Copeland NG, Gilbert DJ, Jenkins NA, et al: Angiopoietins 3 and 4: Diverging gene counterparts in mice and humans. Proc Natl Acad Sci USA. 96:1904–1909. 1999. View Article : Google Scholar : PubMed/NCBI

32 

Plaisier M, Dennert I, Rost E, Koolwijk P, van Hinsbergh VW and Helmerhorst FM: Decidual vascularization and the expression of angiogenic growth factors and proteases in first trimester spontaneous abortions. Hum Reprod. 24:185–197. 2009. View Article : Google Scholar : PubMed/NCBI

33 

Seval Y, Sati L, Celik-Ozenci C, Taskin O and Demir R: The distribution of angiopoietin-1, angiopoietin-2 and their receptors tie-1 and tie-2 in the very early human placenta. Placenta. 29:809–815. 2008. View Article : Google Scholar : PubMed/NCBI

34 

Daponte A, Deligeoroglou E, Pournaras S, Tsezou A, Garas A, Anastasiadou F, Hadjichristodoulou C and Messinis IE: Angiopoietin-1 and angiopoietin-2 as serum biomarkers for ectopic pregnancy and missed abortion: A case-control study. Clin Chim Acta. 415:145–151. 2013. View Article : Google Scholar : PubMed/NCBI

35 

Xiu-Ju MA and Xiao WX: Relationship between oxidative stress and missed abortion. J Shanxi Med Univ. 2006.

36 

Valdivia A, Pérez-Álvarez S, Aroca-Aguilar JD, Ikuta I and Jordán J: Superoxide dismutases: A physiopharmacological update. J Physiol Biochem. 65:195–208. 2009. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Xia S, Zhen Y, Ma H and Wang A: Abnormal expression of microRNA‑575 leads to missed abortion through regulating apoptosis and angiogenesis. Exp Ther Med 14: 3993-4000, 2017.
APA
Xia, S., Zhen, Y., Ma, H., & Wang, A. (2017). Abnormal expression of microRNA‑575 leads to missed abortion through regulating apoptosis and angiogenesis. Experimental and Therapeutic Medicine, 14, 3993-4000. https://doi.org/10.3892/etm.2017.5086
MLA
Xia, S., Zhen, Y., Ma, H., Wang, A."Abnormal expression of microRNA‑575 leads to missed abortion through regulating apoptosis and angiogenesis". Experimental and Therapeutic Medicine 14.5 (2017): 3993-4000.
Chicago
Xia, S., Zhen, Y., Ma, H., Wang, A."Abnormal expression of microRNA‑575 leads to missed abortion through regulating apoptosis and angiogenesis". Experimental and Therapeutic Medicine 14, no. 5 (2017): 3993-4000. https://doi.org/10.3892/etm.2017.5086
Copy and paste a formatted citation
x
Spandidos Publications style
Xia S, Zhen Y, Ma H and Wang A: Abnormal expression of microRNA‑575 leads to missed abortion through regulating apoptosis and angiogenesis. Exp Ther Med 14: 3993-4000, 2017.
APA
Xia, S., Zhen, Y., Ma, H., & Wang, A. (2017). Abnormal expression of microRNA‑575 leads to missed abortion through regulating apoptosis and angiogenesis. Experimental and Therapeutic Medicine, 14, 3993-4000. https://doi.org/10.3892/etm.2017.5086
MLA
Xia, S., Zhen, Y., Ma, H., Wang, A."Abnormal expression of microRNA‑575 leads to missed abortion through regulating apoptosis and angiogenesis". Experimental and Therapeutic Medicine 14.5 (2017): 3993-4000.
Chicago
Xia, S., Zhen, Y., Ma, H., Wang, A."Abnormal expression of microRNA‑575 leads to missed abortion through regulating apoptosis and angiogenesis". Experimental and Therapeutic Medicine 14, no. 5 (2017): 3993-4000. https://doi.org/10.3892/etm.2017.5086
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