Abnormal expression of microRNA‑575 leads to missed abortion through regulating apoptosis and angiogenesis

Xia,Shuqin; Zhen,Yihui; Ma,Hongsheng; Wang,Aiming

doi:10.3892/etm.2017.5086

Abnormal expression of microRNA‑575 leads to missed abortion through regulating apoptosis and angiogenesis

Authors:
- Shuqin Xia
- Yihui Zhen
- Hongsheng Ma
- Aiming Wang
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Affiliations: Department of Gynecology and Obstetrics, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China, Department of Gynecology and Obstetrics, Navy General Hospital PLA China, Beijing 100048, P.R. China
Published online on: August 31, 2017 https://doi.org/10.3892/etm.2017.5086
Pages: 3993-4000
Copyright: © Xia et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Numerous microRNA (miR) are important for placental development and function. miR‑575 has been demonstrated to be upregulated in maternal placenta in patients who have experienced a miscarriage. The present study aimed to explore the role of abnormal expression of miR‑575 in missed abortion (MA) and to further analyze the potential molecular mechanisms. Embryo villus tissue samples were extracted from 10 childless women with MA and 10 fertile women without a history of MA. Additionally, human choriocarcinoma cells, JEG‑3, were used in the present study, which were transfected with miR‑575 mimic, inhibitor and scramble. The expression of miR‑575 in embryo villus tissues and in JEG‑3 cells was detected by reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR). Apoptosis in villus tissues of patients with MA and in JEG‑3 cells of miR‑575 mimic, inhibitor and scramble groups were detected by flow cytometry. Furthermore, the expression levels of apoptosis‑related proteins, including B‑cell lymphoma 2 (Bcl‑2), Bcl‑2‑associated X protein (Bax) and phosphorylated (p)‑p53, and angiogenesis‑related proteins, including vascular endothelial growth factor (VEGF) and angiopoietin 2 (Ang‑2), were measured by RT‑qPCR and western blotting. Additionally, the target of miR‑575 was predicted and clarified by luciferase reporter assay. miR‑575 was significantly overexpressed in MA villus tissue compared with normal tissue (P<0.05). The percentage of apoptotic cells in MA embryo villus tissue was significantly higher than that in normal tissue (P<0.05). After JEG‑3 cells were transfected with miR‑575 inhibitor, the expression of miR‑575 and the percentage of apoptotic cells decreased significantly compared with the control (P<0.05). MiR‑575 suppression significantly increased the expression of Bcl‑2 (P<0.05), and decreased the expressions of Bax (P<0.05) and p‑p53 (P<0.01) compared with the control. Furthermore, miR‑575 suppression significantly increased the expressions of angiogenesis‑related proteins, Ang‑2 and VEGF (P<0.01). Superoxide dismutase 2 was identified as the target of miR‑575. Therefore, abnormal expression of miR‑575 may lead to MA through regulating apoptosis and angiogenesis. Inhibition of miR‑575 may inhibit apoptosis and promote angiogenesis in MA.

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