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Association between B7‑H1 and cervical cancer: B7‑H1 impairs the immune response in human cervical cancer cells

  • Authors:
    • Jianying Tao
    • Jianrong Dai
    • Shunyu Hou
  • View Affiliations / Copyright

    Affiliations: Department of Gynecology and Obstetrics, The Affiliated Suzhou Hospital of Nanjing Medical University, Suzhou, Jiangsu 215006, P.R. China
    Copyright: © Tao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 4125-4133
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    Published online on: September 5, 2017
       https://doi.org/10.3892/etm.2017.5100
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Abstract

The aim of the present study was to determine the preliminary mechanism of action of B7 homolog 1 (B7‑H1) and investigate the association between B7‑H1 and cervical cancer. The expression of B7 family proteins was measured in cervical cancer cells. Cervical cancer cells were co‑cultured with T lymphocytes. An ELISA assay was subsequently conducted to analyze cytokine concentrations in the supernatants of the cultured T cells in cervical cancer cells and B7‑H1 downregulated cells. Levels of interleukin (IL)‑1β, IL‑6 and tumor necrosis factor‑α mRNA in mice injected with cervical cancer cells or B7‑H1 downregulated cells were measured by reverse transcription‑quantitative polymerase chain reaction. It was determined that cervical cancer cells express high levels of B7‑H1, whereas the normal cervical epithelium does not express B7‑H1. When co‑cultured with T lymphocytes, cervical cancer cells were involved in the inhibition of lymphocyte activation. When B7‑H1 was downregulated using a lentivirus, the proliferation ability did not change compared with cervical cancer cells, whereas the soluble factors secreted by T cells differed between cervical cancer cells and B7‑H1 downregulated cells. In an animal model, injected B7‑H1 downregulated cervical cancer cells elicited a more intense immune response, whereas cervical cancer cells had the wild immune response. Therefore, the results of the present study demonstrate that B7‑H1 mediates the low immunogenicity of cervical cancer and is not attacked by the immune system.
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Copy and paste a formatted citation
Spandidos Publications style
Tao J, Dai J and Hou S: Association between B7‑H1 and cervical cancer: B7‑H1 impairs the immune response in human cervical cancer cells. Exp Ther Med 14: 4125-4133, 2017.
APA
Tao, J., Dai, J., & Hou, S. (2017). Association between B7‑H1 and cervical cancer: B7‑H1 impairs the immune response in human cervical cancer cells. Experimental and Therapeutic Medicine, 14, 4125-4133. https://doi.org/10.3892/etm.2017.5100
MLA
Tao, J., Dai, J., Hou, S."Association between B7‑H1 and cervical cancer: B7‑H1 impairs the immune response in human cervical cancer cells". Experimental and Therapeutic Medicine 14.5 (2017): 4125-4133.
Chicago
Tao, J., Dai, J., Hou, S."Association between B7‑H1 and cervical cancer: B7‑H1 impairs the immune response in human cervical cancer cells". Experimental and Therapeutic Medicine 14, no. 5 (2017): 4125-4133. https://doi.org/10.3892/etm.2017.5100
Copy and paste a formatted citation
x
Spandidos Publications style
Tao J, Dai J and Hou S: Association between B7‑H1 and cervical cancer: B7‑H1 impairs the immune response in human cervical cancer cells. Exp Ther Med 14: 4125-4133, 2017.
APA
Tao, J., Dai, J., & Hou, S. (2017). Association between B7‑H1 and cervical cancer: B7‑H1 impairs the immune response in human cervical cancer cells. Experimental and Therapeutic Medicine, 14, 4125-4133. https://doi.org/10.3892/etm.2017.5100
MLA
Tao, J., Dai, J., Hou, S."Association between B7‑H1 and cervical cancer: B7‑H1 impairs the immune response in human cervical cancer cells". Experimental and Therapeutic Medicine 14.5 (2017): 4125-4133.
Chicago
Tao, J., Dai, J., Hou, S."Association between B7‑H1 and cervical cancer: B7‑H1 impairs the immune response in human cervical cancer cells". Experimental and Therapeutic Medicine 14, no. 5 (2017): 4125-4133. https://doi.org/10.3892/etm.2017.5100
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