Lactobacillus raises in vitro anticancer effect of geniposide in HSC‑3 human oral squamous cell carcinoma cells

Cheng,Zhou; Xu,Hui; Wang,Xiaoping; Liu,Zuoye

doi:10.3892/etm.2017.5105

Lactobacillus raises in vitro anticancer effect of geniposide in HSC‑3 human oral squamous cell carcinoma cells

Authors:
- Zhou Cheng
- Hui Xu
- Xiaoping Wang
- Zuoye Liu
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Affiliations: Department of Stomatology, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai, Shandong 264000, P.R. China, Department of Stomatology, Yeda Hospital of Yantai, Yantai, Shandong 264006, P.R. China
Published online on: September 5, 2017 https://doi.org/10.3892/etm.2017.5105
Pages: 4586-4594

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Abstract

The present study determined the ability of the Lactobacillus rhamnosus GG strain (LGG) to enhance the anticancer effects of geniposide on HSC‑3 human oral squamous carcinoma cells. LGG (1.0x103 CFU/ml) on its own had no impact on human oral keratinocytes and HSC‑3 cancer cells. Geniposide (25 or 50 µg/ml) had no impact on human oral keratinocytes, but exerted growth inhibitory effects on HSC‑3 cancer cells, which were increased in the presence of LGG. Flow cytometric analysis and a nuclear staining assay with DAPI revealed that HSC‑3 cancer cells treated with LGG‑geniposide (1.0x103 CFU/ml LGG and 50 µg/ml geniposide) had a higher apoptotic rate than cells in other treatment groups, particularly that treated with geniposide (50 µg/ml) only. Geniposide also increased the mRNA and protein expression of caspase‑3, ‑8 and ‑9 as well as B‑cell lymphoma 2 (Bcl‑2)‑associated X protein, p53, p21, inhibitor of nuclear factor‑κB (NF‑κB) α, Fas and Fas ligand, while decreasing Bcl‑2, Bcl extra large protein, inhibitor of apoptosis‑1 and ‑2, NF‑κB, cyclooxigenase‑2 and inducible nitric oxide synthase in HSC‑3 cells, which was increased in the presence of LGG. These results indicated that LGG enhanced the anticancer effects of geniposide in HSC‑3 cells.

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