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Small interfering RNA against ERK1/2 attenuates cigarette smoke‑induced pulmonary vascular remodeling

  • Authors:
    • Muqing Yu
    • Xiansheng Liu
    • Hongxu Wu
    • Wang Ni
    • Shixin Chen
    • Yongjian Xu
  • View Affiliations / Copyright

    Affiliations: Department of Respiratory and Critical Care Medicine, Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, P.R. China
    Copyright: © Yu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 4671-4680
    |
    Published online on: September 21, 2017
       https://doi.org/10.3892/etm.2017.5160
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Abstract

Cigarette smoke may contribute to pulmonary vascular remodeling (PVR), a result of the proliferation of pulmonary artery smooth muscle cells (PASMCs), before pulmonary hypertension in chronic obstructive pulmonary disease (COPD). Activated extracellular signal‑regulated kinases 1 and 2 (ERK1/2) are considered to be involved the process of PVR. This study investigated the potential role of ERK1/2 in the proliferation of rat PASMCs (rPASMCs) and cigarette smoke‑induced PVR in rats. A small interfering RNA (siRNA) against ERK1/2 (ERK1/2‑siRNA) was synthesized, and it significantly reduced the expression of ERK1/2 and cyclin E1, significantly increased the proportion of cells arrested at G0/G1 phase and significantly suppressed the proliferation of rPASMCs treated with cigarette smoke extract compared with controls (all P<0.05). In rats, ERK1/2‑siRNA, which was administered intranasally, also inhibited the activation of ERK1/2 and the upregulation of cyclin E1, both of which were induced after the rats were exposed to cigarette smoke for 3 months. ERK1/2‑siRNA also significantly reduced PVR (observed by vessel wall thickness and the proportion of fully muscularized vessels) in cigarette smoke‑exposed rats compared with a negative control siRNA (P<0.05). Collectively, these data indicated that ERK1/2‑siRNA could attenuate PVR in cigarette smoke‑exposed rats, and it may have therapeutic value in the treatment of COPD.
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Copy and paste a formatted citation
Spandidos Publications style
Yu M, Liu X, Wu H, Ni W, Chen S and Xu Y: Small interfering RNA against ERK1/2 attenuates cigarette smoke‑induced pulmonary vascular remodeling. Exp Ther Med 14: 4671-4680, 2017.
APA
Yu, M., Liu, X., Wu, H., Ni, W., Chen, S., & Xu, Y. (2017). Small interfering RNA against ERK1/2 attenuates cigarette smoke‑induced pulmonary vascular remodeling. Experimental and Therapeutic Medicine, 14, 4671-4680. https://doi.org/10.3892/etm.2017.5160
MLA
Yu, M., Liu, X., Wu, H., Ni, W., Chen, S., Xu, Y."Small interfering RNA against ERK1/2 attenuates cigarette smoke‑induced pulmonary vascular remodeling". Experimental and Therapeutic Medicine 14.5 (2017): 4671-4680.
Chicago
Yu, M., Liu, X., Wu, H., Ni, W., Chen, S., Xu, Y."Small interfering RNA against ERK1/2 attenuates cigarette smoke‑induced pulmonary vascular remodeling". Experimental and Therapeutic Medicine 14, no. 5 (2017): 4671-4680. https://doi.org/10.3892/etm.2017.5160
Copy and paste a formatted citation
x
Spandidos Publications style
Yu M, Liu X, Wu H, Ni W, Chen S and Xu Y: Small interfering RNA against ERK1/2 attenuates cigarette smoke‑induced pulmonary vascular remodeling. Exp Ther Med 14: 4671-4680, 2017.
APA
Yu, M., Liu, X., Wu, H., Ni, W., Chen, S., & Xu, Y. (2017). Small interfering RNA against ERK1/2 attenuates cigarette smoke‑induced pulmonary vascular remodeling. Experimental and Therapeutic Medicine, 14, 4671-4680. https://doi.org/10.3892/etm.2017.5160
MLA
Yu, M., Liu, X., Wu, H., Ni, W., Chen, S., Xu, Y."Small interfering RNA against ERK1/2 attenuates cigarette smoke‑induced pulmonary vascular remodeling". Experimental and Therapeutic Medicine 14.5 (2017): 4671-4680.
Chicago
Yu, M., Liu, X., Wu, H., Ni, W., Chen, S., Xu, Y."Small interfering RNA against ERK1/2 attenuates cigarette smoke‑induced pulmonary vascular remodeling". Experimental and Therapeutic Medicine 14, no. 5 (2017): 4671-4680. https://doi.org/10.3892/etm.2017.5160
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