Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
November-2017 Volume 14 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
November-2017 Volume 14 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

Identification of key genes influenced by fixation stability in early fracture hematoma and elucidation of their roles in fracture healing

  • Authors:
    • Chengxue Wang
    • Baochang Qi
    • Congfeng Zhang
    • Jieping Cheng
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedic Trauma, The First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China, Department of Orthopedics, The Second People's Hospital of Yushu, Changchun, Jilin 130041, P.R. China, Department of Orthopedics, The Second Hospital of Jilin University, Changchun, Jilin 130041, P.R. China
    Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 4633-4638
    |
    Published online on: September 22, 2017
       https://doi.org/10.3892/etm.2017.5192
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

The present study aimed to identify the key genes influenced by fixation stability in early fracture hematoma and to elucidate their roles in fracture healing. The GSE53256 gene expression profile, including six fracture hematoma tissues, was downloaded from the Gene Expression Omnibus database. The differentially expressed genes (DEGs) in the fracture hematoma tissues from old rats with rigid fixation compared with semi‑rigid fixation were identified using the limma package. Furthermore, Gene Ontology (GO) enrichment analysis for DEGs was performed using BiNGO, and a protein‑protein interaction (PPI) network was constructed based on the Search Tool for the Retrieval of Interacting Genes database. A total of 265 DEGs (158 upregulated and 107 downregulated) in the fracture hematoma tissues were screened out. Additionally, the overrepresented GO terms were mainly associated with the extracellular region, positive regulation of locomotion and response to external stimulus. Transforming growth factor, β 1 (Tgfβ1), chemokine (C‑X‑C motif) ligand 12 (Cxcl12), matrix metallopeptidase 9 (mmp9) and serpin peptidase inhibitor, clade E, member 1 (serpine1) had higher degrees and were hub nodes in the PPI network. In conclusion, fixation stability may influence the fracture healing process, and important DEGs, including Cxcl12, mmp9, Tgfβ1 and serpine1, may be important in this process.
View Figures

Figure 1

Figure 2

Figure 3

View References

1 

Einhorn TA: The cell and molecular biology of fracture healing. Clin Orthop Relat Res. 355 Suppl:S7–S21. 1998. View Article : Google Scholar : PubMed/NCBI

2 

Dimitriou R, Tsiridis E and Giannoudis PV: Current concepts of molecular aspects of bone healing. Injury. 36:1392–1404. 2005. View Article : Google Scholar : PubMed/NCBI

3 

Dimitriou R, Jones E, McGonagle D and Giannoudis PV: Bone regeneration: Current concepts and future directions. BMC Med. 9:662011. View Article : Google Scholar : PubMed/NCBI

4 

Ferguson C, Alpern E, Miclau T and Helms JA: Does adult fracture repair recapitulate embryonic skeletal formation? Mech Dev. 87:57–66. 1999. View Article : Google Scholar : PubMed/NCBI

5 

Strube P, Sentuerk U, Riha T, Kaspar K, Mueller M, Kasper G, Matziolis G, Duda GN and Perka C: Influence of age and mechanical stability on bone defect healing: Age reverses mechanical effects. Bone. 42:758–764. 2008. View Article : Google Scholar : PubMed/NCBI

6 

Mehta M, Strube P, Peters A, Perka C, Hutmacher D, Fratzl P and Duda GN: Influences of age and mechanical stability on volume, microstructure, and mineralization of the fracture callus during bone healing: Is osteoclast activity the key to age-related impaired healing? Bone. 47:219–228. 2010. View Article : Google Scholar : PubMed/NCBI

7 

Epari DR, Kassi JP, Schell H and Duda GN: Timely fracture-healing requires optimization of axial fixation stability. J Bone Joint Surg. 89:1575–1585. 2007. View Article : Google Scholar : PubMed/NCBI

8 

Ranganathan SI, Ferrari M and Decuzzi P: Design maps for scaffold constructs in bone regeneration. Biomed Microdevices. 15:1005–1013. 2013. View Article : Google Scholar : PubMed/NCBI

9 

Kolar P, Schmidt-Bleek K, Schell H, Gaber T, Toben D, Schmidmaier G, Perka C, Buttgereit F and Duda GN: The early fracture hematoma and its potential role in fracture healing. Tissue Eng Part B Rev. 16:427–434. 2010. View Article : Google Scholar : PubMed/NCBI

10 

Oe K, Miwa M, Sakai Y, Lee SY, Kuroda R and Kurosaka M: An in vitro study demonstrating that haematomas found at the site of human fractures contain progenitor cells with multilineage capacity. J Bone Joint Surg Br. 89:133–138. 2007. View Article : Google Scholar : PubMed/NCBI

11 

Street J, Winter D, Wang JH, Wakai A, McGuinness A and Redmond HP: Is human fracture hematoma inherently angiogenic? Clin Orthop Relat Res. 378:224–237. 2000. View Article : Google Scholar

12 

Beamer B, Hettrich C and Lane J: Vascular endothelial growth factor: An essential component of angiogenesis and fracture healing. HSS J. 6:85–94. 2010. View Article : Google Scholar : PubMed/NCBI

13 

Sarahrudi K, Thomas A, Mousavi M, Kaiser G, Köttstorfer J, Kecht M, Hajdu S and Aharinejad S: Elevated transforming growth factor-beta 1 (TGF-β1) levels in human fracture healing. Injury. 42:833–837. 2011. View Article : Google Scholar : PubMed/NCBI

14 

Ode A, Duda GN, Geissler S, Pauly S, Ode JE, Perka C and Strube P: Interaction of age and mechanical stability on bone defect healing: An early transcriptional analysis of fracture hematoma in rat. PLoS One. 9:e1064622014. View Article : Google Scholar : PubMed/NCBI

15 

Gautier L, Cope L, Bolstad BM and Irizarry RA: Affy-analysis of Affymetrix GeneChip data at the probe level. Bioinformatics. 20:307–315. 2004. View Article : Google Scholar : PubMed/NCBI

16 

Smyth GK: Limma: Linear models for microarray dataBioinformatics and Computational Biology Solutions using R and Bioconductor. Gentleman R, Carey VJ, Huber W, Irizarry RA and Dudoit S: Springer; New York: pp. 397–420. 2005, View Article : Google Scholar

17 

Wang L, Cao C, Ma Q, Zeng Q, Wang H, Cheng Z, Zhu G, Qi J, Ma H, Nian H and Wang Y: RNA-seq analyses of multiple meristems of soybean: Novel and alternative transcripts, evolutionary and functional implications. BMC Plant Biol. 14:1692014. View Article : Google Scholar : PubMed/NCBI

18 

Ashburner M, Ball CA, Blake JA, Botstein D, Butler H, Cherry JM, Davis AP, Dolinski K, Dwight SS, Eppig JT, et al: Gene ontology: Tool for the unification of biology. The Gene Ontology Consortium. Nat Genet. 25:25–29. 2000. View Article : Google Scholar : PubMed/NCBI

19 

Maere S, Heymans K and Kuiper M: BiNGO: A Cytoscape plugin to assess overrepresentation of Gene Ontology categories in biological networks. Bioinformatics. 21:3448–3449. 2005. View Article : Google Scholar : PubMed/NCBI

20 

Smoot ME, Ono K, Ruscheinski J, Wang PL and Ideker T: Cytoscape 2.8: New features for data integration and network visualization. Bioinformatics. 27:431–432. 2011. View Article : Google Scholar : PubMed/NCBI

21 

Benjamini Y and Hochberg Y: Controlling the false discovery rate: A practical and powerful approach to multiple testing. J R Stat Soc Series B. 57:289–300. 1995.

22 

Franceschini A, Szklarczyk D, Frankild S, Kuhn M, Simonovic M, Roth A, Lin J, Minguez P, Bork P, von Mering C and Jensen LJ: STRING v9. 1: Protein-protein interaction networks, with increased coverage and integration. Nucleic Acids Res. 41:D808–D815. 2013. View Article : Google Scholar : PubMed/NCBI

23 

Klein P, Schell H, Streitparth F, Heller M, Kassi JP, Kandziora F, Bragulla H, Haas NP and Duda GN: The initial phase of fracture healing is specifically sensitive to mechanical conditions. J Orthop Res. 21:662–669. 2003. View Article : Google Scholar : PubMed/NCBI

24 

Mao L, Kawao N, Tamura Y, Okumoto K, Okada K, Yano M, Matsuo O and Kaji H: Plasminogen activator inhibitor-1 is involved in impaired bone repair associated with diabetes in female mice. PLoS One. 9:e926862014. View Article : Google Scholar : PubMed/NCBI

25 

Galliera E, Corsi M and Banfi G: Platelet rich plasma therapy: Inflammatory molecules involved in tissue healing. J Biol Regul Homeost Agents. 26 2 Suppl 1:35S–42S. 2011.

26 

Honczarenko M, Le Y, Swierkowski M, Ghiran I, Glodek AM and Silberstein LE: Human bone marrow stromal cells express a distinct set of biologically functional chemokine receptors. Stem cells. 24:1030–1041. 2006. View Article : Google Scholar : PubMed/NCBI

27 

Grassi F, Cristino S, Toneguzzi S, Piacentini A, Facchini A and Lisignoli G: CXCL12 chemokine up-regulates bone resorption and MMP-9 release by human osteoclasts: CXCL12 levels are increased in synovial and bone tissue of rheumatoid arthritis patients. J Cell Physiol. 199:244–251. 2004. View Article : Google Scholar : PubMed/NCBI

28 

Pape HC, Marcucio R, Humphrey C, Colnot C, Knobe M and Harvey EJ: Trauma-induced inflammation and fracture healing. J Orthop Trauma. 24:522–525. 2010. View Article : Google Scholar : PubMed/NCBI

29 

Wang X, Yu YY, Lieu S, Yang F, Lang J, Lu C, Werb Z, Hu D, Miclau T, Marcucio R and Colnot C: MMP9 regulates the cellular response to inflammation after skeletal injury. Bone. 52:111–119. 2013. View Article : Google Scholar : PubMed/NCBI

30 

Tang Y, Wu X, Lei W, Pang L, Wan C, Shi Z, Zhao L, Nagy TR, Peng X, Hu J, et al: TGF-beta1-induced migration of bone mesenchymal stem cells couples bone resorption with formation. Nat Med. 15:757–765. 2009. View Article : Google Scholar : PubMed/NCBI

31 

Estai MA, Suhaimi F, Das S, Shuid AN, Mohamed Z and Soelaiman IN: Expression of TGF-β1 in the blood during fracture repair in an estrogen-deficient rat model. Clinics (Sao Paulo). 66:2113–2119. 2011. View Article : Google Scholar : PubMed/NCBI

32 

D'Amelio P, Cristofaro MA, Grimaldi A, Ravazzoli M, Pluviano F, Grosso E, Pescarmona GP and Isaia GC: The role of circulating bone cell precursors in fracture healing. Calcif Tissue Int. 86:463–469. 2010. View Article : Google Scholar : PubMed/NCBI

33 

Sandblrg MM, Aro HT and Vuorio EI: Gene expression during bone repair. Clin Orthop Relat Res. 1–312. 1993.PubMed/NCBI

34 

Vo TN, Kasper FK and Mikos AG: Strategies for controlled delivery of growth factors and cells for bone regeneration. Adv Drug Deliv Rev. 64:1292–1309. 2012. View Article : Google Scholar : PubMed/NCBI

35 

Gils A and Declerck PJ: Plasminogen activators inhibitorsPlasminogen: Structure, Activation and Regulation. Waisman DM: Springer; New York: pp. 47–66. 2003

36 

Declerck PJ and Gils A: Three decades of research on plasminogen activator inhibitor-1: A multifaceted serpin. Semin Thromb Hemost. 39:356–364. 2013. View Article : Google Scholar : PubMed/NCBI

37 

Tamura Y, Kawao N, Okada K, Yano M, Okumoto K, Matsuo O and Kaji H: Plasminogen activator inhibitor-1 is involved in streptozotocin-induced bone loss in female mice. Diabetes. 62:3170–3179. 2013. View Article : Google Scholar : PubMed/NCBI

38 

Rundle CH, Wang X, Wergedal JE, Mohan S and Lau KH: Fracture healing in mice deficient in plasminogen activator inhibitor-1. Calcif Tissue Int. 83:276–284. 2008. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Wang C, Qi B, Zhang C and Cheng J: Identification of key genes influenced by fixation stability in early fracture hematoma and elucidation of their roles in fracture healing. Exp Ther Med 14: 4633-4638, 2017.
APA
Wang, C., Qi, B., Zhang, C., & Cheng, J. (2017). Identification of key genes influenced by fixation stability in early fracture hematoma and elucidation of their roles in fracture healing. Experimental and Therapeutic Medicine, 14, 4633-4638. https://doi.org/10.3892/etm.2017.5192
MLA
Wang, C., Qi, B., Zhang, C., Cheng, J."Identification of key genes influenced by fixation stability in early fracture hematoma and elucidation of their roles in fracture healing". Experimental and Therapeutic Medicine 14.5 (2017): 4633-4638.
Chicago
Wang, C., Qi, B., Zhang, C., Cheng, J."Identification of key genes influenced by fixation stability in early fracture hematoma and elucidation of their roles in fracture healing". Experimental and Therapeutic Medicine 14, no. 5 (2017): 4633-4638. https://doi.org/10.3892/etm.2017.5192
Copy and paste a formatted citation
x
Spandidos Publications style
Wang C, Qi B, Zhang C and Cheng J: Identification of key genes influenced by fixation stability in early fracture hematoma and elucidation of their roles in fracture healing. Exp Ther Med 14: 4633-4638, 2017.
APA
Wang, C., Qi, B., Zhang, C., & Cheng, J. (2017). Identification of key genes influenced by fixation stability in early fracture hematoma and elucidation of their roles in fracture healing. Experimental and Therapeutic Medicine, 14, 4633-4638. https://doi.org/10.3892/etm.2017.5192
MLA
Wang, C., Qi, B., Zhang, C., Cheng, J."Identification of key genes influenced by fixation stability in early fracture hematoma and elucidation of their roles in fracture healing". Experimental and Therapeutic Medicine 14.5 (2017): 4633-4638.
Chicago
Wang, C., Qi, B., Zhang, C., Cheng, J."Identification of key genes influenced by fixation stability in early fracture hematoma and elucidation of their roles in fracture healing". Experimental and Therapeutic Medicine 14, no. 5 (2017): 4633-4638. https://doi.org/10.3892/etm.2017.5192
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team