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Butanol extracts of Asparagus cochinchinensis fermented with Weissella cibaria inhibit iNOS‑mediated COX‑2 induction pathway and inflammatory cytokines in LPS‑stimulated RAW264.7 macrophage cells

  • Authors:
    • Hyun Ah Lee
    • Bo Ram Song
    • Hye Ryeong Kim
    • Ji Eun Kim
    • Woo Bin Yun
    • Jin Ju Park
    • Mi Lim Lee
    • Jun Young Choi
    • Hee Seob Lee
    • Dae Youn Hwang
  • View Affiliations / Copyright

    Affiliations: Department of Biomaterials Science, College of Natural Resources and Life Science/Life and Industry Convergence Research Institute, Pusan National University, Miryang, Gyeongsangnam-do 627‑706, Republic of Korea, Department of Food Science and Nutrition, College of Human Ecology, Pusan National University, Busan 609‑735, Republic of Korea
    Copyright: © Lee et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 4986-4994
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    Published online on: September 25, 2017
       https://doi.org/10.3892/etm.2017.5200
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Abstract

Roots of Asparagus cochinchinesis have been widely used to treat fever, cough, kidney disease, breast cancer, inflammatory and brain disease, although the effects of their fermented products have not been assessed until now. To investigate the anti‑inflammatory effects on macrophages of a butanol extract from A. cochinchinensis roots fermented with Weissella cibaria (BAW), alterations in the inducible nitric oxide synthase (iNOS)‑mediated cyclooxygenase‑2 (COX‑2) induction pathway and inflammatory cytokine expression were measured in lipopolysaccharide (LPS)‑activated RAW264.7 cells following pretreatment with BAW. Briefly, W. cibaria was selected from two bacterial strains for the fermentation of A. cochinchinensis roots based on its hyaluronidase inhibition and NO suppression rates. Following fermentation with W. cibaria, the level of various key components including total phenols and protodioscin were significantly enhanced in BAW. In addition, BAW exhibited high free radical scavenging activity (IC50=31.62 µg/ml) and induced a decrease of intracellular ROS production in RAW264.7 cells following DCFH‑DA staining. Significant suppressions in the expression level of important members of the iNOS‑mediated COX‑2 induction pathway and the phosphorylation of mitogen‑activated protein kinase members were detected. The expressions of pro‑inflammatory and anti‑inflammatory cytokines were recovered in BAW pretreated RAW264.7 cells. Overall, these results suggest that BAW may suppress inflammatory responses through differential regulation of the iNOS‑mediated COX‑2 induction pathway and inflammatory cytokine expressions in LPS‑activated RAW264.7 cells.
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Copy and paste a formatted citation
Spandidos Publications style
Lee HA, Song BR, Kim HR, Kim JE, Yun WB, Park JJ, Lee ML, Choi JY, Lee HS, Hwang DY, Hwang DY, et al: Butanol extracts of Asparagus cochinchinensis fermented with Weissella cibaria inhibit iNOS‑mediated COX‑2 induction pathway and inflammatory cytokines in LPS‑stimulated RAW264.7 macrophage cells. Exp Ther Med 14: 4986-4994, 2017.
APA
Lee, H.A., Song, B.R., Kim, H.R., Kim, J.E., Yun, W.B., Park, J.J. ... Hwang, D.Y. (2017). Butanol extracts of Asparagus cochinchinensis fermented with Weissella cibaria inhibit iNOS‑mediated COX‑2 induction pathway and inflammatory cytokines in LPS‑stimulated RAW264.7 macrophage cells. Experimental and Therapeutic Medicine, 14, 4986-4994. https://doi.org/10.3892/etm.2017.5200
MLA
Lee, H. A., Song, B. R., Kim, H. R., Kim, J. E., Yun, W. B., Park, J. J., Lee, M. L., Choi, J. Y., Lee, H. S., Hwang, D. Y."Butanol extracts of Asparagus cochinchinensis fermented with Weissella cibaria inhibit iNOS‑mediated COX‑2 induction pathway and inflammatory cytokines in LPS‑stimulated RAW264.7 macrophage cells". Experimental and Therapeutic Medicine 14.5 (2017): 4986-4994.
Chicago
Lee, H. A., Song, B. R., Kim, H. R., Kim, J. E., Yun, W. B., Park, J. J., Lee, M. L., Choi, J. Y., Lee, H. S., Hwang, D. Y."Butanol extracts of Asparagus cochinchinensis fermented with Weissella cibaria inhibit iNOS‑mediated COX‑2 induction pathway and inflammatory cytokines in LPS‑stimulated RAW264.7 macrophage cells". Experimental and Therapeutic Medicine 14, no. 5 (2017): 4986-4994. https://doi.org/10.3892/etm.2017.5200
Copy and paste a formatted citation
x
Spandidos Publications style
Lee HA, Song BR, Kim HR, Kim JE, Yun WB, Park JJ, Lee ML, Choi JY, Lee HS, Hwang DY, Hwang DY, et al: Butanol extracts of Asparagus cochinchinensis fermented with Weissella cibaria inhibit iNOS‑mediated COX‑2 induction pathway and inflammatory cytokines in LPS‑stimulated RAW264.7 macrophage cells. Exp Ther Med 14: 4986-4994, 2017.
APA
Lee, H.A., Song, B.R., Kim, H.R., Kim, J.E., Yun, W.B., Park, J.J. ... Hwang, D.Y. (2017). Butanol extracts of Asparagus cochinchinensis fermented with Weissella cibaria inhibit iNOS‑mediated COX‑2 induction pathway and inflammatory cytokines in LPS‑stimulated RAW264.7 macrophage cells. Experimental and Therapeutic Medicine, 14, 4986-4994. https://doi.org/10.3892/etm.2017.5200
MLA
Lee, H. A., Song, B. R., Kim, H. R., Kim, J. E., Yun, W. B., Park, J. J., Lee, M. L., Choi, J. Y., Lee, H. S., Hwang, D. Y."Butanol extracts of Asparagus cochinchinensis fermented with Weissella cibaria inhibit iNOS‑mediated COX‑2 induction pathway and inflammatory cytokines in LPS‑stimulated RAW264.7 macrophage cells". Experimental and Therapeutic Medicine 14.5 (2017): 4986-4994.
Chicago
Lee, H. A., Song, B. R., Kim, H. R., Kim, J. E., Yun, W. B., Park, J. J., Lee, M. L., Choi, J. Y., Lee, H. S., Hwang, D. Y."Butanol extracts of Asparagus cochinchinensis fermented with Weissella cibaria inhibit iNOS‑mediated COX‑2 induction pathway and inflammatory cytokines in LPS‑stimulated RAW264.7 macrophage cells". Experimental and Therapeutic Medicine 14, no. 5 (2017): 4986-4994. https://doi.org/10.3892/etm.2017.5200
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