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Article

GSK126 (EZH2 inhibitor) interferes with ultraviolet A radiation‑induced photoaging of human skin fibroblast cells

  • Authors:
    • Haiyan Qin
    • Guang Zhang
    • Lianbo Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Plastic Surgery, China‑Japan Union Hospital of Jilin University, Changchun, Jilin 130033, P.R. China
  • Pages: 3439-3448
    |
    Published online on: February 13, 2018
       https://doi.org/10.3892/etm.2018.5863
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Abstract

Polycomb group genes (PcG) encode chromatin modification proteins that are involved in the epigenetic regulation of cell differentiation, proliferation and the aging processes. The key subunit of the PcG complex, enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), has a central role in a variety of mechanisms, such as the formation of chromatin structure, gene expression regulation and DNA damage. In the present study, ultraviolet A (UVA) was used to radiate human dermal fibroblasts in order to construct a photo‑aged cell model. Subsequently, the cell viability assay, Hoechst staining, apoptosis detection using flow cytometry, senescence‑associated β‑galactosidase (SA‑β‑gal) staining and erythrocyte exclusion experiments were performed. GSK126, a histone methylation enzyme inhibitor of EZH2, was used as an experimental factor. Results suggested that GSK126 downregulated the mRNA expression levels of EZH2 and upregulated the mRNA expression levels of BMI‑1. Notably, GSK126 affected the transcription of various photoaging‑related genes and thus protected against photoaging induced by UVA radiation.
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Copy and paste a formatted citation
Spandidos Publications style
Qin H, Zhang G and Zhang L: GSK126 (EZH2 inhibitor) interferes with ultraviolet A radiation‑induced photoaging of human skin fibroblast cells. Exp Ther Med 15: 3439-3448, 2018.
APA
Qin, H., Zhang, G., & Zhang, L. (2018). GSK126 (EZH2 inhibitor) interferes with ultraviolet A radiation‑induced photoaging of human skin fibroblast cells. Experimental and Therapeutic Medicine, 15, 3439-3448. https://doi.org/10.3892/etm.2018.5863
MLA
Qin, H., Zhang, G., Zhang, L."GSK126 (EZH2 inhibitor) interferes with ultraviolet A radiation‑induced photoaging of human skin fibroblast cells". Experimental and Therapeutic Medicine 15.4 (2018): 3439-3448.
Chicago
Qin, H., Zhang, G., Zhang, L."GSK126 (EZH2 inhibitor) interferes with ultraviolet A radiation‑induced photoaging of human skin fibroblast cells". Experimental and Therapeutic Medicine 15, no. 4 (2018): 3439-3448. https://doi.org/10.3892/etm.2018.5863
Copy and paste a formatted citation
x
Spandidos Publications style
Qin H, Zhang G and Zhang L: GSK126 (EZH2 inhibitor) interferes with ultraviolet A radiation‑induced photoaging of human skin fibroblast cells. Exp Ther Med 15: 3439-3448, 2018.
APA
Qin, H., Zhang, G., & Zhang, L. (2018). GSK126 (EZH2 inhibitor) interferes with ultraviolet A radiation‑induced photoaging of human skin fibroblast cells. Experimental and Therapeutic Medicine, 15, 3439-3448. https://doi.org/10.3892/etm.2018.5863
MLA
Qin, H., Zhang, G., Zhang, L."GSK126 (EZH2 inhibitor) interferes with ultraviolet A radiation‑induced photoaging of human skin fibroblast cells". Experimental and Therapeutic Medicine 15.4 (2018): 3439-3448.
Chicago
Qin, H., Zhang, G., Zhang, L."GSK126 (EZH2 inhibitor) interferes with ultraviolet A radiation‑induced photoaging of human skin fibroblast cells". Experimental and Therapeutic Medicine 15, no. 4 (2018): 3439-3448. https://doi.org/10.3892/etm.2018.5863
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