Overexpression of growth and differentiation factor-5 inhibits inflammatory factors released by intervertebral disc cells

Shen,Lin; Wu,Yinghua; Han,Liang; Zhang,Haiying

doi:10.3892/etm.2018.5867

Overexpression of growth and differentiation factor-5 inhibits inflammatory factors released by intervertebral disc cells

Authors:
- Lin Shen
- Yinghua Wu
- Liang Han
- Haiying Zhang
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Affiliations: Graduate School, Tianjin Medical University, Tianjin 300070, P.R. China, Department of Orthopedic Trauma, Tianjin Hospital, Tianjin 300211, P.R. China, Department of Orthopedics, Dong Fang Hospital Affiliated to Beijing University of Chinese Medicine, Beijing 100078, P.R. China
Published online on: February 14, 2018 https://doi.org/10.3892/etm.2018.5867
Pages: 3603-3608

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Abstract

Low back pain (LBP) is one of the most common musculoskeletal diseases in the world. The incidence is ~70% in adults and many of them suffer from disability. Recently, intervertebral disc degeneration (IDD) has been deemed as a main cause of LBP. The present study aimed to investigate the potentials of growth and differentiation factor‑5 (GDF‑5) in IDD. The protein levels of prostaglandin‑E2 (PGE2), tumor necrosis factor (TNF)‑α and interleukin (IL)‑1β in culture medium were evaluated by ELISA. mRNA and protein expression levels in nucleus pulposus (NP) cells were evaluated by reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) and western blotting, respectively. Griess reaction was applied to test the nitric oxide (NO) concentration in the culture supernatant. The expression levels of inducible NO synthase (iNOS) and cyclooxygenase‑2 (COX‑2) in NP cells were measured by RT‑qPCR. Collagen‑II, aggrecan, IκBα and phosphorylated (p)‑p65 expression levels were detected by western blotting. Compared with the control group, protein expression levels of TNF‑α, IL‑1β and PGE2, and NO concentration in culture medium were upregulated by LPS, which were significantly repressed by GDF‑5 overexpression (P<0.05). Additionally, GDF‑5 overexpression reduced lipopolysaccharide‑induced upregulation of TNF‑α, IL‑1β, iNOS, COX‑2, collagen‑II, aggrecan, IκBα and p‑p65 expression levels in NP cells.

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