Macrophages ameliorate bone marrow inflammatory injury and promote hematopoiesis in mice following hematopoietic stem cell transplantation

Qiao,Jianlin; Liu,Lu; Xia,Yuan; Ju,Wen; Zhao,Pingping; Jiang,Yan; Li,Mingfeng; Li,Wen; Ding,Lan; Wu,Yulu; Qi,Kunming; Li,Depeng; Zhang,Xi; Xu,Kailin; Zeng,Lingyu

doi:10.3892/etm.2018.6209

August-2018 Volume 16 Issue 2

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August-2018 Volume 16 Issue 2

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Article Open Access

Macrophages ameliorate bone marrow inflammatory injury and promote hematopoiesis in mice following hematopoietic stem cell transplantation

Authors:
- Jianlin Qiao
- Lu Liu
- Yuan Xia
- Wen Ju
- Pingping Zhao
- Yan Jiang
- Mingfeng Li
- Wen Li
- Lan Ding
- Yulu Wu
- Kunming Qi
- Depeng Li
- Xi Zhang
- Kailin Xu
- Lingyu Zeng
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Affiliations: Blood Diseases Institute, Xuzhou Medical University, Xuzhou, Jiangsu 221002, P.R. China, Blood Diseases Institute, Xuzhou Medical University, Xuzhou, Jiangsu 221002, P.R. China, Department of Hematology, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, Jiangsu 221002, P.R. China, Department of Hematology, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, Jiangsu 221002, P.R. China, Department of Hematology, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, P.R. China

Copyright: © Qiao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
Pages: 567-572
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Published online on: May 23, 2018

https://doi.org/10.3892/etm.2018.6209
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Abstract

Bone marrow macrophages have been demonstrated to serve a critical role in promoting maintenance and retention of hematopoietic stem cells (HSCs). Our previous study indicated increased macrophages infiltration in bone marrow after HSC transplantation (HSCT). However, it is not well understood whether macrophages affect hematopoietic reconstitution after HSCT. The present study aimed to investigate the role of macrophages in hematopoietic reconstitution after HSCT. BALB/c mice were divided into HSCT, HSCT+Clodronate Liposomes, HSCT+PBS Liposomes, HSCT+RS102895 and HSCT+Vehicle groups and sacrificed on day 7, 14, 21, 28 and 35 after HSCT. Analysis was performed to detect the changes of bone marrow pathology by H&E staining and the number of macrophages was assessed by immunohistochemical staining and western blot analysis. The number of c‑kit+sca‑1+ and c‑kit+ was measured by flow cytometry. Mice with a depletion of bone marrow macrophages displayed significantly reduced overall survival, delayed hematopoietic recovery, a reduced number of hematopoietic stem/progenitor cells and bone marrow cells as well as exaggerated bone marrow injury. However, compared with the HSCT+Vehicle group, mice with an increased number of bone marrow macrophages exhibited no difference of overall survival, had accelerated hematopoietic reconstitution, a higher number of hematopoietic stem/progenitor cells and bone marrow cells and ameliorated bone marrow injury. In conclusion, the present study indicated that bone marrow macrophages serve a protective role in bone marrow injury and may promote hematopoiesis in mice after HSCT, suggesting manipulation of macrophages may be a novel strategy for improving the efficacy of HSCT.

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1	Vyas P, Appelbaum FR and Craddock C: Allogeneic hematopoietic cell transplantation for acute myeloid leukemia. Biol Blood Marrow Transplant. 21:8–15. 2015. View Article : Google Scholar : PubMed/NCBI
2	Garcia IN: Role of hematopoietic stem cell transplantation in multiple myeloma. Clin Lymphoma Myeloma Leuk. 15:86–91. 2015. View Article : Google Scholar : PubMed/NCBI
3	Danby R and Rocha V: Improving engraftment and immune reconstitution in umbilical cord blood transplantation. Front Immunol. 5:682014. View Article : Google Scholar : PubMed/NCBI
4	Charbord P: Hemopoietic stem cells: Analysis of some parameters critical for engraftment. Stem Cells. 12:545–562. 1994. View Article : Google Scholar : PubMed/NCBI
5	Chang L, Frame D, Braun T, Gatza E, Hanauer DA, Zhao S, Magenau JM, Schultz K, Tokala H, Ferrara JL, et al: Engraftment syndrome after allogeneic hematopoietic cell transplantation predicts poor outcomes. Biol Blood Marrow Transplant. 20:1407–1417. 2014. View Article : Google Scholar : PubMed/NCBI
6	Morrison SJ and Scadden DT: The bone marrow niche for haematopoietic stem cells. Nature. 505:327–334. 2014. View Article : Google Scholar : PubMed/NCBI
7	Yin T and Li L: The stem cell niches in bone. J Clin Invest. 116:1195–1201. 2006. View Article : Google Scholar : PubMed/NCBI
8	Sison EA and Brown P: The bone marrow microenvironment and leukemia: Biology and therapeutic targeting. Expert Rev Hematol. 4:271–283. 2011. View Article : Google Scholar : PubMed/NCBI
9	Hume DA: The mononuclear phagocyte system. Curr Opin Immunol. 18:49–53. 2006. View Article : Google Scholar : PubMed/NCBI
10	Chow A, Lucas D, Hidalgo A, Méndez-Ferrer S, Hashimoto D, Scheiermann C, Battista M, Leboeuf M, Prophete C, van Rooijen N, et al: Bone marrow CD169+ macrophages promote the retention of hematopoietic stem and progenitor cells in the mesenchymal stem cell niche. J Exp Med. 208:261–271. 2011. View Article : Google Scholar : PubMed/NCBI
11	Christopher MJ, Rao M, Liu F, Woloszynek JR and Link DC: Expression of the G-CSF receptor in monocytic cells is sufficient to mediate hematopoietic progenitor mobilization by G-CSF in mice. J Exp Med. 208:251–260. 2011. View Article : Google Scholar : PubMed/NCBI
12	Winkler IG, Sims NA, Pettit AR, Barbier V, Nowlan B, Helwani F, Poulton IJ, van Rooijen N, Alexander KA, Raggatt LJ and Lévesque JP: Bone marrow macrophages maintain hematopoietic stem cell (HSC) niches and their depletion mobilizes HSCs. Blood. 116:4815–4828. 2010. View Article : Google Scholar : PubMed/NCBI
13	Ludin A, Itkin T, Gur-Cohen S, Mildner A, Shezen E, Golan K, Kollet O, Kalinkovich A, Porat Z, D'Uva G, et al: Monocytes-macrophages that express α-smooth muscle actin preserve primitive hematopoietic cells in the bone marrow. Nat Immunol. 13:1072–1082. 2012. View Article : Google Scholar : PubMed/NCBI
14	Qiao J, Wu J, Li Y, Xia Y, Chu P, Qi K, Yan Z, Yao H, Liu Y, Xu K and Zeng L: Blockage of caspase-1 activation ameliorates bone marrow inflammation in mice after hematopoietic stem cell transplantation. Clin Immunol. 162:84–90. 2016. View Article : Google Scholar : PubMed/NCBI
15	Arango Duque G and Descoteaux A: Macrophage cytokines: Involvement in immunity and infectious diseases. Front Immunol. 5:4912014. View Article : Google Scholar : PubMed/NCBI
16	Poon IK, Lucas CD, Rossi AG and Ravichandran KS: Apoptotic cell clearance: Basic biology and therapeutic potential. Nat Rev Immunol. 14:166–180. 2014. View Article : Google Scholar : PubMed/NCBI
17	Zeng L, Ding S, Yan Z, Chen C, Sang W, Cao J, Cheng H and Xu K: Irradiation induces homing of donor endothelial progenitor cells in allogeneic hematopoietic stem cell transplantation. Int J Hematol. 95:189–197. 2012. View Article : Google Scholar : PubMed/NCBI
18	Chen W, Li M, Su G, Zang Y, Yan Z, Cheng H, Pan B, Cao J, Wu Q, Zhao K, et al: Co-transplantation of hematopoietic stem cells and Cxcr4 Gene-transduced mesenchymal stem cells promotes hematopoiesis. Cell Biochem Biophys. 71:1579–1587. 2015. View Article : Google Scholar : PubMed/NCBI
19	van Rooijen N and Hendrikx E: Liposomes for specific depletion of macrophages from organs and tissues. Methods Mol Biol. 605:189–203. 2010. View Article : Google Scholar : PubMed/NCBI

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Macrophages ameliorate bone marrow inflammatory injury and promote hematopoiesis in mice following hematopoietic stem cell transplantation

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