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Dynamic expression of Epac and Rap1 in mouse oocytes and preimplantation embryos

  • Authors:
    • Jun‑Chao Wang
    • Ying Geng
    • Ying Han
    • Hai‑Ning Luo
    • Yun‑Shan Zhang
  • View Affiliations / Copyright

    Affiliations: Center for Reproductive Medicine, Tianjin Central Hospital of Obstetrics and Gynaecology, Tianjin 300100, P.R. China
    Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 523-528
    |
    Published online on: June 6, 2018
       https://doi.org/10.3892/etm.2018.6253
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Abstract

Cyclic adenosine monophosphate (cAMP) is an important secondary messenger that has long been recognized to control the initiation of meiosis through the activation of protein kinase A (PKA) in mammalian oocytes. However, PKA is not the only target for cAMP. Recent studies on cAMP‑dependent and PKA‑independent pathways suggest that Ras‑related protein‑1 (Rap1) is activated through its cAMP‑responsive guanine exchange factors (cAMP‑GEFs), which comprises the involvement of exchange proteins directly activated by cAMP (Epac) in various cellular processes. The aim of the present study was to investigate the possible implication of a cAMP/Epac/Rap1 pathway in mouse oocytes and embryos. Reverse transcription polymerase chain reaction and immunohistochemistry assays demonstrated the expression of Epac and Rap1 in oocytes and embryos at different stages. Immunofluorescene demonstrated that Epac and Rap1 had different dynamic subcellular localizations and expression patterns in oocytes and embryos at different stages. It was therefore indicated that Epac and Rap1 may have multiple and specific functions during oocyte maturation and embryonic development.
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Copy and paste a formatted citation
Spandidos Publications style
Wang JC, Geng Y, Han Y, Luo HN and Zhang YS: Dynamic expression of Epac and Rap1 in mouse oocytes and preimplantation embryos. Exp Ther Med 16: 523-528, 2018.
APA
Wang, J., Geng, Y., Han, Y., Luo, H., & Zhang, Y. (2018). Dynamic expression of Epac and Rap1 in mouse oocytes and preimplantation embryos. Experimental and Therapeutic Medicine, 16, 523-528. https://doi.org/10.3892/etm.2018.6253
MLA
Wang, J., Geng, Y., Han, Y., Luo, H., Zhang, Y."Dynamic expression of Epac and Rap1 in mouse oocytes and preimplantation embryos". Experimental and Therapeutic Medicine 16.2 (2018): 523-528.
Chicago
Wang, J., Geng, Y., Han, Y., Luo, H., Zhang, Y."Dynamic expression of Epac and Rap1 in mouse oocytes and preimplantation embryos". Experimental and Therapeutic Medicine 16, no. 2 (2018): 523-528. https://doi.org/10.3892/etm.2018.6253
Copy and paste a formatted citation
x
Spandidos Publications style
Wang JC, Geng Y, Han Y, Luo HN and Zhang YS: Dynamic expression of Epac and Rap1 in mouse oocytes and preimplantation embryos. Exp Ther Med 16: 523-528, 2018.
APA
Wang, J., Geng, Y., Han, Y., Luo, H., & Zhang, Y. (2018). Dynamic expression of Epac and Rap1 in mouse oocytes and preimplantation embryos. Experimental and Therapeutic Medicine, 16, 523-528. https://doi.org/10.3892/etm.2018.6253
MLA
Wang, J., Geng, Y., Han, Y., Luo, H., Zhang, Y."Dynamic expression of Epac and Rap1 in mouse oocytes and preimplantation embryos". Experimental and Therapeutic Medicine 16.2 (2018): 523-528.
Chicago
Wang, J., Geng, Y., Han, Y., Luo, H., Zhang, Y."Dynamic expression of Epac and Rap1 in mouse oocytes and preimplantation embryos". Experimental and Therapeutic Medicine 16, no. 2 (2018): 523-528. https://doi.org/10.3892/etm.2018.6253
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