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Development of zoledronic acid functionalized hydroxyapatite loaded polymeric nanoparticles for the treatment of osteoporosis

  • Authors:
    • Yunlu Cai
    • Tiantian Gao
    • Shiping Fu
    • Ping Sun
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedics, The Eighth People's Hospital of Shanghai, Shanghai 200235, P.R. China
    Copyright: © Cai et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 704-710
    |
    Published online on: June 7, 2018
       https://doi.org/10.3892/etm.2018.6263
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Abstract

The present study formulated a hydroxyapatite (HA)‑coated lipid nanoparticle loaded with zoledronic acid to treat patients with osteoporosis (OP). HA‑coated zoledronic acid loaded lipid bilayer nanoparticles (HZL NPs) were prepared using methods of extrusion and precipitation. Nanosized particles were prepared with the aim of increasing gradual and prolonged drug release and inducing toxicity of osteoblasts. Cellular morphology was investigated by scanning electron microscopy, which revealed clear spherical shaped NPs ~200 nm in size that could treat osteopores in the bone. In addition, a typical biphasic release pattern was observed that could be attributed to the presence of drug on the outer surface as well as on the inner core of the NPs. There was sustained release behavior [38.17±2.12% (pH 7.4) and 64.2±3.75% (pH 5) at 48 h] of the drug that maintained the drug reservoir effect at the bone site. Furthermore, HZL NPs increased the cytotoxicity of HFOb 1.19 cells and increased the proportion of cells in the early (18.1±12.4%), late (28.7±3.7%) and necrotic (67.5±1.2%) phases of apoptosis. Most importantly, HZL in the lipid nanoparticle exhibited a strong affinity towards HA, further enhancing its efficacy in the treatment of OP.
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Copy and paste a formatted citation
Spandidos Publications style
Cai Y, Gao T, Fu S and Sun P: Development of zoledronic acid functionalized hydroxyapatite loaded polymeric nanoparticles for the treatment of osteoporosis. Exp Ther Med 16: 704-710, 2018.
APA
Cai, Y., Gao, T., Fu, S., & Sun, P. (2018). Development of zoledronic acid functionalized hydroxyapatite loaded polymeric nanoparticles for the treatment of osteoporosis. Experimental and Therapeutic Medicine, 16, 704-710. https://doi.org/10.3892/etm.2018.6263
MLA
Cai, Y., Gao, T., Fu, S., Sun, P."Development of zoledronic acid functionalized hydroxyapatite loaded polymeric nanoparticles for the treatment of osteoporosis". Experimental and Therapeutic Medicine 16.2 (2018): 704-710.
Chicago
Cai, Y., Gao, T., Fu, S., Sun, P."Development of zoledronic acid functionalized hydroxyapatite loaded polymeric nanoparticles for the treatment of osteoporosis". Experimental and Therapeutic Medicine 16, no. 2 (2018): 704-710. https://doi.org/10.3892/etm.2018.6263
Copy and paste a formatted citation
x
Spandidos Publications style
Cai Y, Gao T, Fu S and Sun P: Development of zoledronic acid functionalized hydroxyapatite loaded polymeric nanoparticles for the treatment of osteoporosis. Exp Ther Med 16: 704-710, 2018.
APA
Cai, Y., Gao, T., Fu, S., & Sun, P. (2018). Development of zoledronic acid functionalized hydroxyapatite loaded polymeric nanoparticles for the treatment of osteoporosis. Experimental and Therapeutic Medicine, 16, 704-710. https://doi.org/10.3892/etm.2018.6263
MLA
Cai, Y., Gao, T., Fu, S., Sun, P."Development of zoledronic acid functionalized hydroxyapatite loaded polymeric nanoparticles for the treatment of osteoporosis". Experimental and Therapeutic Medicine 16.2 (2018): 704-710.
Chicago
Cai, Y., Gao, T., Fu, S., Sun, P."Development of zoledronic acid functionalized hydroxyapatite loaded polymeric nanoparticles for the treatment of osteoporosis". Experimental and Therapeutic Medicine 16, no. 2 (2018): 704-710. https://doi.org/10.3892/etm.2018.6263
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