Baicalin attenuates liver hypoxia/reoxygenation injury by inducing autophagy

Liu,Feng; Zhang,Jing; Qian,Jianmin; Wu,Gang; Ma,Zhenyu

doi:10.3892/etm.2018.6284

Baicalin attenuates liver hypoxia/reoxygenation injury by inducing autophagy

Authors:
- Feng Liu
- Jing Zhang
- Jianmin Qian
- Gang Wu
- Zhenyu Ma
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Affiliations: Department of Integrative Medicine, Huashan Hospital, Fudan University, Shanghai 200040, P.R. China, Nursing Center, Huashan Hospital, Fudan University, Shanghai 200040, P.R. China, Department of General Surgery, Huashan Hospital, Fudan University, Shanghai 200040, P.R. China
Published online on: June 8, 2018 https://doi.org/10.3892/etm.2018.6284
Pages: 657-664
Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The aim of the present study was to explore the effect of baicalin on liver hypoxia/reoxygenation (H/R) injury and the possible mechanism involved. A cellular H/R model was established and cells were treated with 50, 100 and 200 µmol/l baicalin. Following reoxygenation for 6 h, cell viability, lactate dehydrogenase (LDH), B‑cell lymphoma 2 (Bcl‑2), Bcl‑2‑associated X protein (Bax), caspase 3 and cleaved caspase 3 were assessed. Furthermore, levels of endoplasmic reticulum stress markers binding of immunoglobulin protein (BIP) and CCAAT/enhancer‑binding protein homologous protein (CHOP) and autophagy markers microtubule‑associated proteins 1A/1B light chain 3B (LC3) and beclin 1 were measured. To confirm the involvement of autophagy in baicalin‑mediated attenuation of H/R injury, the autophagy inhibitor 3‑methyladenine (3‑MA) was administered. The results revealed that baicalin administration increased cell viability and decreased LDH levels, most notably at a dosage of 100 µmol/l. Baicalin pretreatment also downregulated the expression of caspase 3, cleaved caspase 3 and Bax, while upregulating the expression of Bcl‑2. Furthermore, BIP and CHOP were decreased while LC3 and beclin‑1 were significantly increased by baicalin pretreatment. Inhibiting autophagy using 3‑MA, resulted in a significant decrease in LC3‑II, beclin‑1 and LDH, as well as increase in the expression of BIP, CHOP, caspase 3, cleaved caspase 3 and Bax. Bcl‑2 and cell viability were also decreased. In conclusion, the results of the present study indicate that baicalin exerts a protective effect on liver H/R injury and this may be achieved via the induction of autophagy.

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