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Effects of ginsenoside Rb1 on oxidative stress injury in rat spinal cords by regulating the eNOS/Nrf2/HO‑1 signaling pathway

  • Authors:
    • Xinwei Liu
    • Xiaochuan Gu
    • Miaomiao Yu
    • Ying Zi
    • Hailong Yu
    • Yu Wang
    • Yanchun Xie
    • Liangbi Xiang
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedics, Rescue Center of Severe Wound and Trauma of the PLA, General Hospital of Shenyang Military Area Command, Shenyang, Liaoning 110016, P.R. China, Department of Orthopedics, Changhai Hospital Αffiliated to The Second Military Medical University, Shanghai 200433, P.R. China, Department of Emergency, Hospital 463 of the PLA, Shenyang, Liaoning 110042, P.R. China
    Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1079-1086
    |
    Published online on: June 12, 2018
       https://doi.org/10.3892/etm.2018.6286
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Abstract

The present study aimed to investigate whether ginsenoside Rb1 (G‑Rb1) attenuates spinal cord injury‑associated oxidative stress in rats by regulating the endothelial nitric oxide synthase eNOS/nuclear factor erythroid 2‑related factor 2 (Nrf2)/heme oxygenase (HO)‑1 signaling pathway. Sprague Dawley rats were randomly divided into the sham operation group (S group), spinal cord injury group (SCI group), G‑Rb1 treatment group (G‑Rb1 group) and SCI+G‑Rb1+Inhibitor L‑name group (L‑name group). The posterior limb function was evaluated via the Basso, Beattie and Bresnahan scoring method. The levels of superoxide dismutase (SOD), malondialdehyde (MDA), catalase (CAT) and glutathione (GSH) in serum were measured by ELISA. The pathological changes in the spinal cord were observed by H&E staining. Reverse transcription‑quantitative polymerase chain reaction and western blot analyses were used to detect eNOS, phosphorylated (p)‑eNOS, heat shock protein (HSP)90, Nrf2 and NAD(P)H quinone dehydrogenase 1 (Nqo1) at the mRNA and protein level. Immunohistochemistry was used to detect the expression of Nrf2 and p‑eNOS. Compared with the S group, the scores of spinal cord function in the SCI group were significantly lower, and the levels of MDA were significantly increased, while the levels of SOD, CAT and GSH protein in spinal cord were significantly decreased (P<0.05). The spinal cord tissue exhibited hemorrhage, neuronal degeneration/necrosis, as well as mononuclear cell and lymphocyte infiltration. The eNOS, HSP90, Nrf2, Nqo1 and HO‑1 mRNA levels were decreased (P<0.05). Compared with those in the SCI group, the spinal cord function score in the G‑Rb1 group were significantly higher and the serum MDA content was significantly decreased, while the activity of SOD, CAT and GSH was significantly increased (P<0.05). The degeneration/necrosis of spinal cord neurons was attenuated, inflammatory cell infiltration was significantly reduced and the levels of eNOS, HSP90, Nrf2, Nqo1 and HO‑1 were significantly upregulated (P<0.05). In the group that was administered the eNOS inhibitor L‑name, the levels of eNOS, HSP90, Nrf2, Nqo1 and HO‑1 were significantly decreased. In conclusion, G‑Rb1 attenuates oxidative stress in injured spinal cords. The mechanism may at least in part involve the eNOS/Nrf2/HO‑1 pathway.
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Copy and paste a formatted citation
Spandidos Publications style
Liu X, Gu X, Yu M, Zi Y, Yu H, Wang Y, Xie Y and Xiang L: Effects of ginsenoside Rb1 on oxidative stress injury in rat spinal cords by regulating the eNOS/Nrf2/HO‑1 signaling pathway. Exp Ther Med 16: 1079-1086, 2018.
APA
Liu, X., Gu, X., Yu, M., Zi, Y., Yu, H., Wang, Y. ... Xiang, L. (2018). Effects of ginsenoside Rb1 on oxidative stress injury in rat spinal cords by regulating the eNOS/Nrf2/HO‑1 signaling pathway. Experimental and Therapeutic Medicine, 16, 1079-1086. https://doi.org/10.3892/etm.2018.6286
MLA
Liu, X., Gu, X., Yu, M., Zi, Y., Yu, H., Wang, Y., Xie, Y., Xiang, L."Effects of ginsenoside Rb1 on oxidative stress injury in rat spinal cords by regulating the eNOS/Nrf2/HO‑1 signaling pathway". Experimental and Therapeutic Medicine 16.2 (2018): 1079-1086.
Chicago
Liu, X., Gu, X., Yu, M., Zi, Y., Yu, H., Wang, Y., Xie, Y., Xiang, L."Effects of ginsenoside Rb1 on oxidative stress injury in rat spinal cords by regulating the eNOS/Nrf2/HO‑1 signaling pathway". Experimental and Therapeutic Medicine 16, no. 2 (2018): 1079-1086. https://doi.org/10.3892/etm.2018.6286
Copy and paste a formatted citation
x
Spandidos Publications style
Liu X, Gu X, Yu M, Zi Y, Yu H, Wang Y, Xie Y and Xiang L: Effects of ginsenoside Rb1 on oxidative stress injury in rat spinal cords by regulating the eNOS/Nrf2/HO‑1 signaling pathway. Exp Ther Med 16: 1079-1086, 2018.
APA
Liu, X., Gu, X., Yu, M., Zi, Y., Yu, H., Wang, Y. ... Xiang, L. (2018). Effects of ginsenoside Rb1 on oxidative stress injury in rat spinal cords by regulating the eNOS/Nrf2/HO‑1 signaling pathway. Experimental and Therapeutic Medicine, 16, 1079-1086. https://doi.org/10.3892/etm.2018.6286
MLA
Liu, X., Gu, X., Yu, M., Zi, Y., Yu, H., Wang, Y., Xie, Y., Xiang, L."Effects of ginsenoside Rb1 on oxidative stress injury in rat spinal cords by regulating the eNOS/Nrf2/HO‑1 signaling pathway". Experimental and Therapeutic Medicine 16.2 (2018): 1079-1086.
Chicago
Liu, X., Gu, X., Yu, M., Zi, Y., Yu, H., Wang, Y., Xie, Y., Xiang, L."Effects of ginsenoside Rb1 on oxidative stress injury in rat spinal cords by regulating the eNOS/Nrf2/HO‑1 signaling pathway". Experimental and Therapeutic Medicine 16, no. 2 (2018): 1079-1086. https://doi.org/10.3892/etm.2018.6286
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