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Article

Activation of protein kinase C‑α/heme oxygenase‑1 signaling pathway improves mitochondrial dynamics in lipopolysaccharide‑activated NR8383 cells

  • Authors:
    • Xiangyun Li
    • Yuan Zhang
    • Jianbo Yu
    • Rui Mu
    • Lili Wu
    • Jia Shi
    • Lirong Gong
    • Daquan Liu
  • View Affiliations / Copyright

    Affiliations: Department of Anesthesiology, Tianjin Nankai Hospital, Tianjin Medical University, Tianjin 300100, P.R. China, Department of Pharmacology, Institute of Integrated Traditional Chinese and Western Medicine for Acute Abdominal Diseases, Tianjin 300100, P.R. China
  • Pages: 1529-1537
    |
    Published online on: June 12, 2018
       https://doi.org/10.3892/etm.2018.6290
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Abstract

Mitochondrial function and morphology are dynamically regulated by fusion and fission. Heme oxygenase‑1 (HO‑1), which may be upregulated by protein kinase C‑α (PKC‑α), improves mitochondrial dynamics by controlling the balance between fusion and fission in vivo and in vitro. However, whether the PKC‑α/HO‑1 signaling pathway is one of the underlying mechanisms in adjusting mitochondrial dynamics in lipopolysaccharide (LPS)‑activated macrophages has remained elusive. To explore this, NR8383 cells were pre‑treated with PKC‑α inhibitor Go6976 or PKC‑α activator phorbol‑12‑myristate‑13‑acetate for 30 min and then stimulated with LPS for 24 h. Next, the expression of PKC‑α, HO‑1, mitofusin 1 (Mfn1) and mitofusin 2 (Mfn2), optic atrophy 1 (OPA1), dynamin‑related protein 1 (Drp1) and fission 1 (Fis1) was detected to evaluate the possible implication of the PKC‑α/HO‑1 signaling pathway in the LPS‑induced NR8383 cells. The results indicated that activation of the PKC‑α/HO‑1 signaling pathway increased superoxide dismutase activities and the respiratory control ratio (RCR), decreased the levels of malondialdehyde, reactive oxygen species (ROS), Drp1 and Fis1, and simultaneously enhanced the levels of Mfn1, Mfn2 and OPA1. In contrast, the PKC‑α inhibitor decreased the expression of RCR, Mfn1, Mfn2 and OPA1, and increased the expression of MDA and ROS in NR8383 cells. The results suggest that activation of the PKC‑α/HO‑1 signaling pathway is necessary for the balance of mitochondrial dynamics and oxidative stress in macrophages, which provides clues for probing novel strategies against the detrimental effects of sepsis and other disease states.
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Copy and paste a formatted citation
Spandidos Publications style
Li X, Zhang Y, Yu J, Mu R, Wu L, Shi J, Gong L and Liu D: Activation of protein kinase C‑α/heme oxygenase‑1 signaling pathway improves mitochondrial dynamics in lipopolysaccharide‑activated NR8383 cells. Exp Ther Med 16: 1529-1537, 2018.
APA
Li, X., Zhang, Y., Yu, J., Mu, R., Wu, L., Shi, J. ... Liu, D. (2018). Activation of protein kinase C‑α/heme oxygenase‑1 signaling pathway improves mitochondrial dynamics in lipopolysaccharide‑activated NR8383 cells. Experimental and Therapeutic Medicine, 16, 1529-1537. https://doi.org/10.3892/etm.2018.6290
MLA
Li, X., Zhang, Y., Yu, J., Mu, R., Wu, L., Shi, J., Gong, L., Liu, D."Activation of protein kinase C‑α/heme oxygenase‑1 signaling pathway improves mitochondrial dynamics in lipopolysaccharide‑activated NR8383 cells". Experimental and Therapeutic Medicine 16.2 (2018): 1529-1537.
Chicago
Li, X., Zhang, Y., Yu, J., Mu, R., Wu, L., Shi, J., Gong, L., Liu, D."Activation of protein kinase C‑α/heme oxygenase‑1 signaling pathway improves mitochondrial dynamics in lipopolysaccharide‑activated NR8383 cells". Experimental and Therapeutic Medicine 16, no. 2 (2018): 1529-1537. https://doi.org/10.3892/etm.2018.6290
Copy and paste a formatted citation
x
Spandidos Publications style
Li X, Zhang Y, Yu J, Mu R, Wu L, Shi J, Gong L and Liu D: Activation of protein kinase C‑α/heme oxygenase‑1 signaling pathway improves mitochondrial dynamics in lipopolysaccharide‑activated NR8383 cells. Exp Ther Med 16: 1529-1537, 2018.
APA
Li, X., Zhang, Y., Yu, J., Mu, R., Wu, L., Shi, J. ... Liu, D. (2018). Activation of protein kinase C‑α/heme oxygenase‑1 signaling pathway improves mitochondrial dynamics in lipopolysaccharide‑activated NR8383 cells. Experimental and Therapeutic Medicine, 16, 1529-1537. https://doi.org/10.3892/etm.2018.6290
MLA
Li, X., Zhang, Y., Yu, J., Mu, R., Wu, L., Shi, J., Gong, L., Liu, D."Activation of protein kinase C‑α/heme oxygenase‑1 signaling pathway improves mitochondrial dynamics in lipopolysaccharide‑activated NR8383 cells". Experimental and Therapeutic Medicine 16.2 (2018): 1529-1537.
Chicago
Li, X., Zhang, Y., Yu, J., Mu, R., Wu, L., Shi, J., Gong, L., Liu, D."Activation of protein kinase C‑α/heme oxygenase‑1 signaling pathway improves mitochondrial dynamics in lipopolysaccharide‑activated NR8383 cells". Experimental and Therapeutic Medicine 16, no. 2 (2018): 1529-1537. https://doi.org/10.3892/etm.2018.6290
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