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Analysis of the miRNA and mRNA involved in osteogenesis of adipose‑derived mesenchymal stem cells

  • Authors:
    • Bo Jia
    • Zhaoqiang Zhang
    • Xiaoling Qiu
    • Hongxing Chu
    • Xiang Sun
    • Xianghuai Zheng
    • Jianjiang Zhao
    • Qin Li
  • View Affiliations / Copyright

    Affiliations: Laboratory for Oral Diseases, Department of Oral and Maxillofacial Surgery, Stomatological Hospital, Southern Medical University, Guangzhou, Guangdong 510280, P.R. China, Guangzhou School of Clinical Medicine, Southern Medical University, Guangzhou General Hospital of Guangzhou Military Region, Guangzhou, Guangdong 510010, P.R. China
    Copyright: © Jia et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1111-1120
    |
    Published online on: June 13, 2018
       https://doi.org/10.3892/etm.2018.6303
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Abstract

Mesenchymal stem cells (MSCs) are bone marrow stromal cells capable of differentiating into different tissue types. Osteoblastic differentiation is a complex process that is critical for bone formation. An increasing number of studies have suggested that microRNAs (miRNAs) may serve important roles in various biological processes, including osteogenesis of MSCs. However, less is known about the participation of particular miRNAs in the osteogenic differentiation of adipose‑derived stem cells (ADSCs). In order to identify functional miRNAs and the key genes involved in the osteogenesis of MSCs, the present study reconstructed a global network using data from the National Center for Biotechnology Information Gene Expression Omnibus. Meanwhile, gene ontology and pathway analysis were performed using the Cytoscape plug‑in BinGO and the Database for Annotation, Visualization, and Integration Discovery, respectively. An miRNA‑mRNA network composed of 72 mRNA and nine miRNA nodes advised by bioinformatics analysis was constructed. These mRNAs and miRNAs were predicted to be involved in the regulation of osteogenic differentiation of ADSCs according to the gene microarray. In the present study, six miRNAs (miR‑143‑3p, miR‑135a‑5p, miR‑31‑5p, miR‑22‑3p, miR‑193b‑3p and let‑7i‑5p) were observed to be highly associated with the osteogenesis of ADSCs, and dihydropyrimidinase like 3 was identified as a novel regulator in this process. These results provide support for further investigations into the management of bone regeneration‑associated diseases.
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Copy and paste a formatted citation
Spandidos Publications style
Jia B, Zhang Z, Qiu X, Chu H, Sun X, Zheng X, Zhao J and Li Q: Analysis of the miRNA and mRNA involved in osteogenesis of adipose‑derived mesenchymal stem cells. Exp Ther Med 16: 1111-1120, 2018.
APA
Jia, B., Zhang, Z., Qiu, X., Chu, H., Sun, X., Zheng, X. ... Li, Q. (2018). Analysis of the miRNA and mRNA involved in osteogenesis of adipose‑derived mesenchymal stem cells. Experimental and Therapeutic Medicine, 16, 1111-1120. https://doi.org/10.3892/etm.2018.6303
MLA
Jia, B., Zhang, Z., Qiu, X., Chu, H., Sun, X., Zheng, X., Zhao, J., Li, Q."Analysis of the miRNA and mRNA involved in osteogenesis of adipose‑derived mesenchymal stem cells". Experimental and Therapeutic Medicine 16.2 (2018): 1111-1120.
Chicago
Jia, B., Zhang, Z., Qiu, X., Chu, H., Sun, X., Zheng, X., Zhao, J., Li, Q."Analysis of the miRNA and mRNA involved in osteogenesis of adipose‑derived mesenchymal stem cells". Experimental and Therapeutic Medicine 16, no. 2 (2018): 1111-1120. https://doi.org/10.3892/etm.2018.6303
Copy and paste a formatted citation
x
Spandidos Publications style
Jia B, Zhang Z, Qiu X, Chu H, Sun X, Zheng X, Zhao J and Li Q: Analysis of the miRNA and mRNA involved in osteogenesis of adipose‑derived mesenchymal stem cells. Exp Ther Med 16: 1111-1120, 2018.
APA
Jia, B., Zhang, Z., Qiu, X., Chu, H., Sun, X., Zheng, X. ... Li, Q. (2018). Analysis of the miRNA and mRNA involved in osteogenesis of adipose‑derived mesenchymal stem cells. Experimental and Therapeutic Medicine, 16, 1111-1120. https://doi.org/10.3892/etm.2018.6303
MLA
Jia, B., Zhang, Z., Qiu, X., Chu, H., Sun, X., Zheng, X., Zhao, J., Li, Q."Analysis of the miRNA and mRNA involved in osteogenesis of adipose‑derived mesenchymal stem cells". Experimental and Therapeutic Medicine 16.2 (2018): 1111-1120.
Chicago
Jia, B., Zhang, Z., Qiu, X., Chu, H., Sun, X., Zheng, X., Zhao, J., Li, Q."Analysis of the miRNA and mRNA involved in osteogenesis of adipose‑derived mesenchymal stem cells". Experimental and Therapeutic Medicine 16, no. 2 (2018): 1111-1120. https://doi.org/10.3892/etm.2018.6303
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