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The preparation and clinical application of diagnostic DNA microarray for the detection of pathogens in intracranial bacterial and fungal infections

  • Authors:
    • Jingrong Cao
    • Shichao Gao
    • Jing Chen
    • Bin Zhu
    • Rong Min
    • Peichang Wang
  • View Affiliations / Copyright

    Affiliations: Department of Clinical Laboratory, Xuanwu Hospital of Capital Medical University, Beijing 100053, P.R. China, Department of Microbiology, Health Occupation College of Jiangxi, Nanchang, Jiangxi 330201, P.R. China, Department of Technical Quality, Shanghai BaiO Technology Co., Ltd., Shanghai 200233, P.R. China
    Copyright: © Cao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1304-1310
    |
    Published online on: June 14, 2018
       https://doi.org/10.3892/etm.2018.6312
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Abstract

The present study prepared 2 types of DNA diagnostic chips based on 16S ribosomal DNA (rDNA) and 18S‑28S rDNA, and evaluated their values in the detection of pathogens in intracranial bacterial/fungal infections. A total of 14 probes of bacteria (Klebsiella pneumonia, Acinetobacter baumannii, Pseudomonas aeruginosa, Escherichia coli, Haemophilus influenza, Stenotrophomonas maltophilia, Neisseria meningitidis, Enterobacter spp., Enterococcus faecalis, Enterococcus faecium, Listeria monocytogenes, Staphylococcus aureus, Streptococcus pneumonia and coagulase negative staphylococcus) and 4 probes of fungi (Candida albicans, Candida tropicalis, Candida glabrata and Cryptococcus neoformans), determined frequently in cerebrospinal fluid (CSF), were designed and used for preparation of microarrays. CSF samples from 88 patients with clinically suspected intracranial infection and standard strains were used to evaluate the chips. The same samples were also analyzed by culture and sequencing. The results demonstrated that the sensitivity, specificity and false‑positive rate of the microarray assay compared with culture method were 100 vs. 68.3% (P<0.05), 97.1 vs. 100%, and 2.9 vs. 0%, respectively. The minimum concentration of detection with the chips was 10 cfu ml‑1 for bacteria and 100 cfu ml‑1 for fungi. The specificity of the probes was confirmed, and no cross‑reaction was detected in the present study. Furthermore, 13 cases were positive in the microarray and negative in culture. However, 4 cases were not identified as clear pathogens and only positive in the 16S probe sites. The diagnostic DNA microarray for intracranial infections has proven to be more rapid and sensitive, and it may be a better option for clinical application than culture methods.
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Copy and paste a formatted citation
Spandidos Publications style
Cao J, Gao S, Chen J, Zhu B, Min R and Wang P: The preparation and clinical application of diagnostic DNA microarray for the detection of pathogens in intracranial bacterial and fungal infections. Exp Ther Med 16: 1304-1310, 2018.
APA
Cao, J., Gao, S., Chen, J., Zhu, B., Min, R., & Wang, P. (2018). The preparation and clinical application of diagnostic DNA microarray for the detection of pathogens in intracranial bacterial and fungal infections. Experimental and Therapeutic Medicine, 16, 1304-1310. https://doi.org/10.3892/etm.2018.6312
MLA
Cao, J., Gao, S., Chen, J., Zhu, B., Min, R., Wang, P."The preparation and clinical application of diagnostic DNA microarray for the detection of pathogens in intracranial bacterial and fungal infections". Experimental and Therapeutic Medicine 16.2 (2018): 1304-1310.
Chicago
Cao, J., Gao, S., Chen, J., Zhu, B., Min, R., Wang, P."The preparation and clinical application of diagnostic DNA microarray for the detection of pathogens in intracranial bacterial and fungal infections". Experimental and Therapeutic Medicine 16, no. 2 (2018): 1304-1310. https://doi.org/10.3892/etm.2018.6312
Copy and paste a formatted citation
x
Spandidos Publications style
Cao J, Gao S, Chen J, Zhu B, Min R and Wang P: The preparation and clinical application of diagnostic DNA microarray for the detection of pathogens in intracranial bacterial and fungal infections. Exp Ther Med 16: 1304-1310, 2018.
APA
Cao, J., Gao, S., Chen, J., Zhu, B., Min, R., & Wang, P. (2018). The preparation and clinical application of diagnostic DNA microarray for the detection of pathogens in intracranial bacterial and fungal infections. Experimental and Therapeutic Medicine, 16, 1304-1310. https://doi.org/10.3892/etm.2018.6312
MLA
Cao, J., Gao, S., Chen, J., Zhu, B., Min, R., Wang, P."The preparation and clinical application of diagnostic DNA microarray for the detection of pathogens in intracranial bacterial and fungal infections". Experimental and Therapeutic Medicine 16.2 (2018): 1304-1310.
Chicago
Cao, J., Gao, S., Chen, J., Zhu, B., Min, R., Wang, P."The preparation and clinical application of diagnostic DNA microarray for the detection of pathogens in intracranial bacterial and fungal infections". Experimental and Therapeutic Medicine 16, no. 2 (2018): 1304-1310. https://doi.org/10.3892/etm.2018.6312
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