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Effect of NMDA on proliferation and apoptosis in hippocampal neural stem cells treated with MK‑801

  • Authors:
    • Juan Ding
    • Yu Shao
    • Hui‑Hui Zhou
    • Quan‑Rui Ma
    • Yi‑Wei Zhang
    • Yin‑Xiu Ding
    • Yu‑Qing He
    • Juan Liu

  • View Affiliations

  • Published online on: June 22, 2018     https://doi.org/10.3892/etm.2018.6346
  • Pages: 1137-1142

  • Copyright: © Ding et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The purpose of the present study was to investigate effects of N‑methyl‑D‑aspartate (NMDA) on proliferation and apoptosis of hippocampal neural stem cells (NSCs) treated with dizocilpine (MK‑801). Cultures of hippocampal NSCs were randomly divided into four groups consisting of an untreated control, cells treated with MK‑801, NMDA and a combination of MK801 and NMDA (M+N). Proliferative and apoptotic responses for each of the experimental groups were determined by MTS and flow cytometry. The results revealed that MK‑801 and NMDA exerted significant effects on hippocampal NSCs proliferation. Cell survival rates decreased in MK‑801, NMDA and M+N treated groups compared with the control group. Cells survival rates in NMDA and M+N treated groups increased compared with the MK‑801 treated group. MK‑801 and NMDA were demonstrated to significantly affect apoptosis in hippocampal NSCs. Total and early stages of apoptosis in MK‑801 and NMDA groups significantly increased compared with the control group. Total and early apoptosis of NSCs in the M+N group significantly decreased compared with MK‑801 and NMDA groups. Late apoptosis of NSCs in MK‑801 and NMDA groups significantly decreased compared with the control group. Late apoptosis of NSCs in the M+N group significantly increased compared with MK‑801 and NMDA groups. The present study revealed that MK‑801 inhibited proliferation and increased apoptosis in hippocampal NSCs. NMDA may reduce the neurotoxicity induced by MK‑801, which may be associated with its activity towards NMDA receptors and may describe a novel therapeutic target for the treatment of schizophrenia.
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August-2018
Volume 16 Issue 2

Print ISSN: 1792-0981
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Spandidos Publications style
Ding J, Shao Y, Zhou HH, Ma QR, Zhang YW, Ding YX, He YQ and Liu J: Effect of NMDA on proliferation and apoptosis in hippocampal neural stem cells treated with MK‑801. Exp Ther Med 16: 1137-1142, 2018
APA
Ding, J., Shao, Y., Zhou, H., Ma, Q., Zhang, Y., Ding, Y. ... Liu, J. (2018). Effect of NMDA on proliferation and apoptosis in hippocampal neural stem cells treated with MK‑801. Experimental and Therapeutic Medicine, 16, 1137-1142. https://doi.org/10.3892/etm.2018.6346
MLA
Ding, J., Shao, Y., Zhou, H., Ma, Q., Zhang, Y., Ding, Y., He, Y., Liu, J."Effect of NMDA on proliferation and apoptosis in hippocampal neural stem cells treated with MK‑801". Experimental and Therapeutic Medicine 16.2 (2018): 1137-1142.
Chicago
Ding, J., Shao, Y., Zhou, H., Ma, Q., Zhang, Y., Ding, Y., He, Y., Liu, J."Effect of NMDA on proliferation and apoptosis in hippocampal neural stem cells treated with MK‑801". Experimental and Therapeutic Medicine 16, no. 2 (2018): 1137-1142. https://doi.org/10.3892/etm.2018.6346