Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
September-2018 Volume 16 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
September-2018 Volume 16 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

Dynamic expression of matrix metalloproteinases 2, 9 and 13 in ovariectomy‑induced osteoporosis rats

  • Authors:
    • Xuefeng Zheng
    • Yuanyuan Zhang
    • Shiming Guo
    • Wenming Zhang
    • Jinyun Wang
    • Yanping Lin
  • View Affiliations / Copyright

    Affiliations: Acupuncture College, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350122, P.R. China, Orthopedics Department, Zhangzhou Hospital of Traditional Chinese Medicine, Zhangzhou, Fujian 363000, P.R. China, Orthopedics Department, Luoyang Osteopathy Hospital, Luoyang, Henan 471002, P.R. China
    Copyright: © Zheng et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1807-1813
    |
    Published online on: June 27, 2018
       https://doi.org/10.3892/etm.2018.6356
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

The aim of the present study was to examine the dynamic expression of matrix metalloproteinase (MMP)‑2, MMP‑9 and MMP‑13 in an ovariectomy (OVX)‑induced osteoporosis rat model. A total of 80 Sprague‑Dawley female rats (age, 3 months) were randomly divided into the OVX and sham groups, with 40 rats in each group. Rats in the sham group received sham surgery, while the remaining rats were ovariectomized. After 12, 16, 20 and 24 weeks, 10 rats from each group were randomly sacrificed, respectively. It was observed that the bone mineral density (BMD) and the trabecular bone area in the OVX group were significantly lower as compared with those in the sham group (P<0.01). The expression levels of MMP‑2 and MMP‑9 were negatively correlated with the BMD, while MMP‑13 was positively correlated with the BMD. The expression levels of MMP‑2 and MMP‑9 increased more abruptly and were significant higher in the OVX group in comparison with those in the sham group between 12 and 24 weeks after surgery (P<0.01). More specifically, the MMP‑9 mRNA expression level in the OVX group increased abruptly between 12 and 24 weeks after surgery. By contrast, in the sham group, the MMP‑9 mRNA level was undetectable between 12 and 16 weeks, and increased steadily between 16 and 24 weeks. Furthermore, the mRNA and protein expression levels of MMP‑13 initially increased and then decreased in the OVX group (P<0.01 vs. the sham group), whereas they continuously increased in the sham group between 12 and 24 weeks after surgery. In conclusion, MMP‑2, MMP‑9 and MMP‑13 regulated the development of osteoporosis, and MMP‑9 may be used as an important marker in the early diagnosis of osteoporosis.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

View References

1 

Kanis JA: Diagnosis of osteoporosis and assessment of fracture risk. Lancet. 359:1929–1936. 2002. View Article : Google Scholar : PubMed/NCBI

2 

Hohenhaus MH, McGarry KA and Col NF: Hormone therapy for the prevention of bone loss in menopausal women with osteopenia: Is it a viable option? Drugs. 67:2311–2321. 2007. View Article : Google Scholar : PubMed/NCBI

3 

Levine JP: Effective strategies to identify postmenopausal women at risk for osteoporosis. Geriatrics. 62:22–30. 2007.PubMed/NCBI

4 

Krane SM and Inada M: Matrix metalloproteinases and bone. Bone. 43:7–18. 2008. View Article : Google Scholar : PubMed/NCBI

5 

Mott JD and Werb Z: Regulation of matrix biology by matrix metalloproteinases. Curr Opin Cell Biol. 16:558–564. 2004. View Article : Google Scholar : PubMed/NCBI

6 

Andersen TL, del Carmen Ovejero M, Kirkegaard T, Lenhard T, Foged NT and Delaissé JM: A scrutiny of matrix metalloproteinases in osteoclasts: Evidence for heterogeneity and for the presence of MMPs synthesized by other cell. Bone. 35:1107–1119. 2004. View Article : Google Scholar : PubMed/NCBI

7 

Tezuka K, Nemoto K, Tezuka Y, Sato T, Ikeda Y, Kobori M, Kawashima H, Eguchi H, Hakeda Y and Kumegawa M: Identification of matrix metalloproteinase-9 in rabbit osteoclasts. J Biol Chem. 269:15006–15009. 1994.PubMed/NCBI

8 

Inoue K, Mikuni-Takagaki Y, Oikawa K, Itoh T, Inada M, Noguchi T, Park JS, Onodera T, Krane SM, Noda M and Itohara S: A crucial role for matrix metalloproteinase 2 in osteocytic canalicular formation and bone metabolism. J Biol Chem. 281:33814–33824. 2006. View Article : Google Scholar : PubMed/NCBI

9 

Engsig MT, Chen QJ, Vu TH, Pedersen AC, Therkidsen B, Lund LR, Henriksen K, Lenhard T, Foged NT, Werb Z and Delaissé JM: Matrix metalloproteinase 9 and vascular endothelial growth factor are essential for osteoclast recruitment into developing long bones. J Cell Biol. 151:879–889. 2000. View Article : Google Scholar : PubMed/NCBI

10 

Varghese S and Canalis E: Alendronate stimulates collagenase 3 expression in osteoblasts by posttranscriptional mechanisms. J Bone Miner Res. 15:2345–235l. 2000. View Article : Google Scholar : PubMed/NCBI

11 

Sun B, Sun J, Han X, Liu H, Li J, Du J, Feng W, Liu B, Cui J, Guo J, et al: Immunolocalization of MMP 2, 9 and 13 in prednisolone induced osteoporosis in mice. Histol Histopathol. 31:647–656. 2016.PubMed/NCBI

12 

Tang SY, Herber RP, Ho SP and Alliston T: Matrix metalloproteinase-13 is required for osteocytic perilacunar remodeling and maintains bone fracture resistance. J Bone Miner Res. 27:1936–1950. 2012. View Article : Google Scholar : PubMed/NCBI

13 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

14 

Egermann M, Goldhahn J and Schneider E: Animal models for fracture treatment inosteoporosis. Osteoporosis Int. 16 Suppl 2:S129–S138. 2005. View Article : Google Scholar

15 

Samanna V, Ma T, Mak TW, Rogers M and Chellaiah MA: Actin polymerization modulates CD44 surface expression, MMP-9 activation, and osteoclast function. J Cell Physiol. 213:710–720. 2007. View Article : Google Scholar : PubMed/NCBI

16 

Zhang P and Zhong M: Effects of 17beta-estradiol and progesterone on the expression of matrix metalloproteinases and tissue inhibitors of metalloproteinases in rat osteoblasts. Di Yi Jun Yi Da Xue Xue Bao. 21:929–931. 2001.PubMed/NCBI

17 

Bachmeier BE, Iancu CM, Jochum M and Nerlich AG: Matrix metalloproteinases in cancer: Comparison of known and novel aspects of their inhibition as a therapeutic approach. Expe Rev Anticancer Ther. 5:149–163. 2005. View Article : Google Scholar

18 

Martignetti JA, Aqeel AA, Sewairi WA, Boumah CE, Kambouris M, Mayouf SA, Sheth KV, Eid WA, Dowling O, Harris J, et al: Mutation of the matrix metalloproteinase 2 gene (MMP2) causes a multicentric osteolysis and arthritis syndrome. Nat Genet. 28:261–265. 2001. View Article : Google Scholar : PubMed/NCBI

19 

Nyman JS, Lynch CC, Perrien DS, Thiolloy S, O'Quinn EC, Patil CA, Bi X, Pharr GM, Mahadevan-Jansen A and Mundy GR: Differential effects between the loss of MMP-2 and MMP-9 on structural and tissue-level properties of bone. J Bone Miner Res. 26:1252–1260. 2011. View Article : Google Scholar : PubMed/NCBI

20 

Zhao H, Cai G, Du J, Xia Z, Wang L and Zhu T: Expression of matrix metalloproteinase-9 mRNA in osteoporotic bone tissues. J Tongji Med Univ. 17:28–31. 1997. View Article : Google Scholar : PubMed/NCBI

21 

Filanti C, Dickson GR, Di Martino D, Ulivi V, Sanguineti C, Romano P, Palermo C and Manduca P: The expression of metalloproteinase-2, −9, and −14 and of tissue inhibitors-1 and −2 is developmentally modulated during osteogenesis in vitro, the mature osteoblastic phenotype expressing metalloproteinase-14. J Bone Miner Res. 15:2154–2168. 2000. View Article : Google Scholar : PubMed/NCBI

22 

Jemtlan R, Holden M, Reppe S, Olstad OK, Reinholt FP, Gautvik VT, Refvem H, Frigessi A, Houston B and Gautvik KM: Molecular disease map of bone characterizing the postmenopausal osteoporosis phenotype. J Bone Miner Res. 26:1793–1801. 2011. View Article : Google Scholar : PubMed/NCBI

23 

Pivetta E, Scapolan M, Pecolo M, Wassermann B, Abu-Rumeileh I, Balestreri L, Borsatti E, Tripodo C, Colombatti A and Spessotto P: MMP-13 stimulates osteoclast differentiation and activation in tumour breast bone metastases. Breast Cancer Res. 13:R1052011. View Article : Google Scholar : PubMed/NCBI

24 

Li J, Liao EY, Dai RC, Wei QY and Luo XH: Effects of 17 beta-estradiol on the expression of interstitial collagenases-8 and −13 (MMP-8 and MMP-13) and tissue inhibitor of metalloproteinase-1 (TIMP-1) in ovariectomized rat osteoblastic cells. J Mol Histol. 35:723–731. 2004. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Zheng X, Zhang Y, Guo S, Zhang W, Wang J and Lin Y: Dynamic expression of matrix metalloproteinases 2, 9 and 13 in ovariectomy‑induced osteoporosis rats. Exp Ther Med 16: 1807-1813, 2018.
APA
Zheng, X., Zhang, Y., Guo, S., Zhang, W., Wang, J., & Lin, Y. (2018). Dynamic expression of matrix metalloproteinases 2, 9 and 13 in ovariectomy‑induced osteoporosis rats. Experimental and Therapeutic Medicine, 16, 1807-1813. https://doi.org/10.3892/etm.2018.6356
MLA
Zheng, X., Zhang, Y., Guo, S., Zhang, W., Wang, J., Lin, Y."Dynamic expression of matrix metalloproteinases 2, 9 and 13 in ovariectomy‑induced osteoporosis rats". Experimental and Therapeutic Medicine 16.3 (2018): 1807-1813.
Chicago
Zheng, X., Zhang, Y., Guo, S., Zhang, W., Wang, J., Lin, Y."Dynamic expression of matrix metalloproteinases 2, 9 and 13 in ovariectomy‑induced osteoporosis rats". Experimental and Therapeutic Medicine 16, no. 3 (2018): 1807-1813. https://doi.org/10.3892/etm.2018.6356
Copy and paste a formatted citation
x
Spandidos Publications style
Zheng X, Zhang Y, Guo S, Zhang W, Wang J and Lin Y: Dynamic expression of matrix metalloproteinases 2, 9 and 13 in ovariectomy‑induced osteoporosis rats. Exp Ther Med 16: 1807-1813, 2018.
APA
Zheng, X., Zhang, Y., Guo, S., Zhang, W., Wang, J., & Lin, Y. (2018). Dynamic expression of matrix metalloproteinases 2, 9 and 13 in ovariectomy‑induced osteoporosis rats. Experimental and Therapeutic Medicine, 16, 1807-1813. https://doi.org/10.3892/etm.2018.6356
MLA
Zheng, X., Zhang, Y., Guo, S., Zhang, W., Wang, J., Lin, Y."Dynamic expression of matrix metalloproteinases 2, 9 and 13 in ovariectomy‑induced osteoporosis rats". Experimental and Therapeutic Medicine 16.3 (2018): 1807-1813.
Chicago
Zheng, X., Zhang, Y., Guo, S., Zhang, W., Wang, J., Lin, Y."Dynamic expression of matrix metalloproteinases 2, 9 and 13 in ovariectomy‑induced osteoporosis rats". Experimental and Therapeutic Medicine 16, no. 3 (2018): 1807-1813. https://doi.org/10.3892/etm.2018.6356
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team