Open Access

MicroRNA‑138 inhibits SOX12 expression and the proliferation, invasion and migration of ovarian cancer cells

  • Authors:
    • Miaomiao Qu
    • Yongning Zhu
    • Meng Jin
  • View Affiliations

  • Published online on: June 29, 2018     https://doi.org/10.3892/etm.2018.6375
  • Pages: 1629-1638
  • Copyright: © Qu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The aim of the present study was to investigate the expression and biological functions of microRNA (miR)‑138 in ovarian cancer at the tissue and cellular levels, as well as its underlying mechanisms. A total of 47 patients with ovarian cancer were included in the present study. Ovarian cancer tissues were subjected to staging classification according to the FIGO 2000 criteria. Lymphatic metastasis was also examined. Ovarian cancer A2780 cells were transfected using liposomes. Reverse transcription‑quantitative polymerase chain reaction was used to measure the expression of miR‑138. A Cell‑Counting Kit 8 assay was used to examine cell viability, while a Transwell assay was employed to study cell invasion and migration. The effects of miR‑138 on SOX12 protein expression were examined by western blot analysis. A dual luciferase reporter assay was performed to identify the direct interaction between miR‑138 and SOX12 gene. Expression of miR‑138 was downregulated in ovarian cancer tissues. The level of miR‑138 in patients with ovarian cancer with lymphatic metastasis was significantly lower compared with patients without lymphatic metastasis. However, expression of miR‑138 was not associated with the stage of ovarian cancer. Upregulation of miR‑138 inhibited the proliferation and suppressed the invasion and migration of A2780 cells. SOX12 promoted the proliferation, invasion and migration of A2780 cells. In addition, miR‑138 downregulated the expression of SOX12 via binding with the 3'‑UTR of SOX12 gene. The present study demonstrates that miR‑138 expression is downregulated in ovarian cancer tissues and miR‑138 acts as a tumor suppressor gene by inhibiting SOX12 expression and the proliferation, invasion and migration of ovarian cancer cells.
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September-2018
Volume 16 Issue 3

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Copy and paste a formatted citation
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Spandidos Publications style
Qu M, Zhu Y and Jin M: MicroRNA‑138 inhibits SOX12 expression and the proliferation, invasion and migration of ovarian cancer cells. Exp Ther Med 16: 1629-1638, 2018.
APA
Qu, M., Zhu, Y., & Jin, M. (2018). MicroRNA‑138 inhibits SOX12 expression and the proliferation, invasion and migration of ovarian cancer cells. Experimental and Therapeutic Medicine, 16, 1629-1638. https://doi.org/10.3892/etm.2018.6375
MLA
Qu, M., Zhu, Y., Jin, M."MicroRNA‑138 inhibits SOX12 expression and the proliferation, invasion and migration of ovarian cancer cells". Experimental and Therapeutic Medicine 16.3 (2018): 1629-1638.
Chicago
Qu, M., Zhu, Y., Jin, M."MicroRNA‑138 inhibits SOX12 expression and the proliferation, invasion and migration of ovarian cancer cells". Experimental and Therapeutic Medicine 16, no. 3 (2018): 1629-1638. https://doi.org/10.3892/etm.2018.6375