Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
September-2018 Volume 16 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
September-2018 Volume 16 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

Fmr1 protects cardiomyocytes against lipopolysaccharide‑induced myocardial injury

  • Authors:
    • Jiasheng Bao
    • Chen Ye
    • Zhelan Zheng
    • Zhengwen Zhou
  • View Affiliations / Copyright

    Affiliations: Department of Electrocardiogram Diagnosis, Zhejiang Hospital, Hangzhou, Zhejiang 310013, P.R. China, Department of Cardiovascular Medicine, Zhejiang Hospital, Hangzhou, Zhejiang 310013, P.R. China, Department of Cardiovascular Ultrasonic Center, The First Affiliated Hospital of Zhejiang University, The First Hospital of Zhejiang Province, Hangzhou, Zhejiang 310003, P.R. China
    Copyright: © Bao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1825-1833
    |
    Published online on: July 2, 2018
       https://doi.org/10.3892/etm.2018.6386
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

The present study explored the mechanisms by which fragile X mental retardation 1 (fmr1) overexpression inhibits lipopolysaccharide (LPS)‑induced cardiomyocyte injury. Factors including oxidative stress reaction, mitochondrial membrane potential variation and cell apoptosis were evaluated. The viability of H9c2 cells was evaluated with a Cell Counting Kit‑8 assay after cells were treated with LPS at different concentrations (0, 1, 3, 6 and 9 µg/ml) for various durations (4, 12 and 24 h). Flow cytometry was used to determine variations in reactive oxygen species (ROS), mitochondrial membrane potential and cell apoptosis. Reverse transcription‑quantitative polymerase chain reaction and western blot analysis were performed to detect the levels of apoptosis‑associated factors, and western blot analysis was used to determine the phosphorylation levels of phosphoinositide‑3 kinase (PI3K), Akt and forkhead box (Fox)O3a. The results indicated that LPS decreased the viability of H9c2 cells in a dose‑ and time‑dependent manner. Overexpression of fmr1 inhibited the LPS‑induced decrease in the mitochondrial membrane potential and the production of ROS as well as apoptosis in H9c2 cells. Fmr1 also inhibited LPS‑induced reductions in antioxidant enzyme activities, including those of superoxide dismutase and reduced/oxidized glutathione ratio, and decreased LPS‑associated increases in the lipid peroxidation product malondialdehyde. Apoptosis‑associated factors were identified to be involved in the effects of Fmr1. Overexpression of Fmr1 attenuated LPS‑associated increases in the apoptosis‑activating factors B‑cell lymphoma 2 (Bcl‑2)‑associated X protein and caspase‑3 and decreases in apoptosis inhibitors, including Bcl‑2 and X‑linked inhibitor of apoptosis protein. Fmr1 overexpression also reduced LPS‑induced increases in the phosphorylation levels of PI3K, Akt and FoxO3a. In conclusion, fmr1 overexpression alleviated oxidative stress and apoptosis in H9c2 cardiomyocytes injured by LPS via regulating oxidative stress and apoptosis‑associated factors, as well as the PI3K/Akt pathway. This information may provide a novel and effective therapeutic strategy for heart diseases.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

View References

1 

Fattahi F and Ward PA: Complement and sepsis-induced heart dysfunction. Mol Immunol. 84:57–64. 2017. View Article : Google Scholar : PubMed/NCBI

2 

Murphy E and Steenbergen C: What makes the mitochondria a killer? Can we condition them to be less destructive? Biochim Biophys Acta. 1813:1302–1308. 2011. View Article : Google Scholar : PubMed/NCBI

3 

Chen JQ, Cammarata PR, Baines CP and Yager JD: Regulation of mitochondrial respiratory chain biogenesis by estrogens/estrogen receptors and physiological, pathological and pharmacological implications. Biochim Biophys Acta. 1793:1540–1570. 2009. View Article : Google Scholar : PubMed/NCBI

4 

Lin WJ and Yeh WC: Implication of Toll-like receptor and tumor necrosis factor alpha signaling in septic shock. Shock. 24:206–209. 2005. View Article : Google Scholar : PubMed/NCBI

5 

Vieillard-Baron A, Caille V, Charron C, Belliard G, Page B and Jardin F: Actual incidence of global left ventricular hypokinesia in adult septic shock. Crit Care Med. 36:1701–1706. 2008. View Article : Google Scholar : PubMed/NCBI

6 

Niederbichler AD, Westfall MV, Su GL, Donnerberg J, Usman A, Vogt PM, Ipaktchi KR, Arbabi S, Wang SC and Hemmila MR: Cardiomyocyte function after burn injury and lipopolysaccharide exposure: Single-cell contraction analysis and cytokine secretion profile. Shock. 25:176–183. 2006. View Article : Google Scholar : PubMed/NCBI

7 

Akira S, Takeda K and Kaisho T: Toll-like receptors: Critical proteins linking innate and acquired immunity. Nat Immunol. 2:675–680. 2001. View Article : Google Scholar : PubMed/NCBI

8 

Van't Padje S, Chaudhry B, Severijnen LA, van der Linde HC, Mientjes EJ, Oostra BA and Willemsen R: Reduction in fragile X related 1 protein causes cardiomyopathy and muscular dystrophy in zebrafish. J Exp Biol. 212:2564–2570. 2009. View Article : Google Scholar : PubMed/NCBI

9 

Jiraanont P, Hagerman RJ, Neri G, Zollino M, Murdolo M and Tassone F: Germinal mosaicism for a deletion of the FMR1 gene leading to fragile X syndrome. Eur J Med Genet. 59:459–462. 2016. View Article : Google Scholar : PubMed/NCBI

10 

Novak SM, Joardar A, Gregorio CC and Zarnescu DC: Regulation of heart rate in drosophila via fragile X mental retardation protein. PLoS One. 10:e01428362015. View Article : Google Scholar : PubMed/NCBI

11 

Klusek J, Roberts JE and Losh M: Cardiac autonomic regulation in autism and Fragile X syndrome: A review. Psychol Bull. 141:141–175. 2015. View Article : Google Scholar : PubMed/NCBI

12 

Schechter MA, Southerland KW, Feger BJ, Linder D Jr, Ali AA, Njoroge L, Milano CA and Bowles DE: An isolated working heart system for large animal models. J Vis Exp. 2014. View Article : Google Scholar : PubMed/NCBI

13 

Chen L, Li W, Qi D and Wang D: Lycium barbarum polysaccharide protects against LPS-induced ARDS by inhibiting apoptosis, oxidative stress, and inflammation in pulmonary endothelial cells. Free Radic Res. 23:1–11. 2018.

14 

Dong Z and Yuan Y: Accelerated inflammation and oxidative stress induced by LPS in acute lung injury: Inhibition by ST1926. Int J Mol Med. 41:3405–3421. 2018.PubMed/NCBI

15 

Zhang H, Zhang W, Jiao F, Li X, Zhang H, Wang L and Gong Z: The nephroprotective effect of MS-275 on lipopolysaccharide (LPS)-induced acute kidney injury by inhibiting reactive oxygen species (ROS)-oxidative stress and endoplasmic reticulum stress. Med Sci Monit. 24:2620–2630. 2018. View Article : Google Scholar : PubMed/NCBI

16 

Tavares AM, da Rosa Araujo AS, Llesuy S, Khaper N, Rohde LE, Clausell N and Belló-Klein A: Early loss of cardiac function in acute myocardial infarction is associated with redox imbalance. Exp Clin Cardiol. 17:263–267. 2012.PubMed/NCBI

17 

Selvaraju V, Joshi M, Suresh S, Sanchez JA, Maulik N and Maulik G: Diabetes, oxidative stress, molecular mechanism, and cardiovascular disease-an overview. Toxicol Mech Methods. 22:330–335. 2012. View Article : Google Scholar : PubMed/NCBI

18 

Deng C, Sun Z, Tong G, Yi W, Ma L, Zhao B, Cheng L, Zhang J, Cao F and Yi D: α-Lipoic acid reduces infarct size and preserves cardiac function in rat myocardial ischemia/reperfusion injury through activation of PI3K/Akt/Nrf2 pathway. PLoS One. 8:e583712013. View Article : Google Scholar : PubMed/NCBI

19 

Ma Y, Tian S, Wang Z, Wang C, Chen X, Li W, Yang Y and He S: CMP-N-acetylneuraminic acid synthetase interacts with fragile X related protein 1. Mol Med Rep. 14:1501–1508. 2016. View Article : Google Scholar : PubMed/NCBI

20 

Buja Maximilian L: Mitochondria in ischemic heart disease. Adv Exp Med Biol. 982:127–140. 2017. View Article : Google Scholar : PubMed/NCBI

21 

Wang X, Lai R, Su X, Chen G and Liang Z: Edaravone attenuates lipopolysaccharide-induced acute respiratory distress syndrome associated early pulmonary fibrosis via inhibition of oxidative stress and transforming growth factor-β1/Smad3 signaling. Biochem Biophys Res Commun. 495:706–712. 2018. View Article : Google Scholar : PubMed/NCBI

22 

Ding ZM, Jiao XF, Wu D, Zhang JY, Chen F, Wang YS, Huang CJ, Zhang SX, Li X and Huo LJ: Bisphenol AF negatively affects oocyte maturation of mouse in vitro through increasing oxidative stress and DNA damage. Chem Biol Interact. 278:222–229. 2017. View Article : Google Scholar : PubMed/NCBI

23 

Gölz L, Memmert S, Rath-Deschner B, Jäger A, Appel T, Baumgarten G, Gotz W and Frede S: LPS from P. gingivalis and hypoxia increases oxidative stress in periodontal ligament fibroblasts and contributes to periodontitis. Mediators Inflamm. 2014:9862642014. View Article : Google Scholar : PubMed/NCBI

24 

Liu XR, Li T, Cao L, Yu YY, Chen LL, Fan XH, Yang BB and Tan XQ: Dexmedetomidine attenuates H2O2-induced neonatal rat cardiomyocytes apoptosis through mitochondria- and ER-medicated oxidative stress pathways. Mol Med Rep. 17:7258–7264. 2018.PubMed/NCBI

25 

Wang Y, Lei T, Yuan J, Wu Y, Shen X, Gao J, Feng W and Lu Z: GCN2 deficiency ameliorates doxorubicin-induced cardiotoxicity by decreasing cardiomyocyte apoptosis and myocardial oxidative stress. Redox Biol. 17:25–34. 2018. View Article : Google Scholar : PubMed/NCBI

26 

Wang Z, Wang M, Liu J, Ye J, Jiang H, Xu Y, Ye D and Wan J: Inhibition of TRPA1 attenuates doxorubicin-induced acute cardiotoxicity by suppressing oxidative stress, the inflammatory response, and endoplasmic reticulum stress. Oxid Med Cell Longev. 2018:51794682018. View Article : Google Scholar : PubMed/NCBI

27 

Li D, Qu Y, Mao M, Zhang X, Li J, Ferriero D and Mu D: Involvement of the PTEN-AKT-FOXO3a pathway in neuronal apoptosis in developing rat brain after hypoxia-ischemia. J Cereb Blood Flow Metab. 29:1903–1913. 2009. View Article : Google Scholar : PubMed/NCBI

28 

Nishina A, Shimizu K, Koketsu M, Ninomiya M, Sato D, Suzuki T, Hayakawa S and Kimura H: 5,7-Dihydroxyflavone analogues may regulate lipopolysaccharide-induced inflammatory responses by suppressing iκbα-linked AKT and ERK5 phosphorylation in raw 264.7 macrophages. Evid Based Compl Altern Med. 2017:78989732017.

29 

Shi J, Shan S, Li H, Song G and Li Z: Anti-inflammatory effects of millet bran derived-bound polyphenols in LPS-induced HT-29 cell via ROS/miR-149/Akt/NF-κB signaling pathway. Oncotarget. 8:74582–74594. 2017.PubMed/NCBI

30 

Wu DP, Zhang JL, Wang JY, Cui MX, Jia JL, Liu XH and Liang QD: MiR-1246 promotes LPS-induced inflammatory injury in chondrogenic cells ATDC5 by targeting HNF4γ. Cell Physiol Biochem. 43:2010–2021. 2017. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Bao J, Ye C, Zheng Z and Zhou Z: Fmr1 protects cardiomyocytes against lipopolysaccharide‑induced myocardial injury. Exp Ther Med 16: 1825-1833, 2018.
APA
Bao, J., Ye, C., Zheng, Z., & Zhou, Z. (2018). Fmr1 protects cardiomyocytes against lipopolysaccharide‑induced myocardial injury. Experimental and Therapeutic Medicine, 16, 1825-1833. https://doi.org/10.3892/etm.2018.6386
MLA
Bao, J., Ye, C., Zheng, Z., Zhou, Z."Fmr1 protects cardiomyocytes against lipopolysaccharide‑induced myocardial injury". Experimental and Therapeutic Medicine 16.3 (2018): 1825-1833.
Chicago
Bao, J., Ye, C., Zheng, Z., Zhou, Z."Fmr1 protects cardiomyocytes against lipopolysaccharide‑induced myocardial injury". Experimental and Therapeutic Medicine 16, no. 3 (2018): 1825-1833. https://doi.org/10.3892/etm.2018.6386
Copy and paste a formatted citation
x
Spandidos Publications style
Bao J, Ye C, Zheng Z and Zhou Z: Fmr1 protects cardiomyocytes against lipopolysaccharide‑induced myocardial injury. Exp Ther Med 16: 1825-1833, 2018.
APA
Bao, J., Ye, C., Zheng, Z., & Zhou, Z. (2018). Fmr1 protects cardiomyocytes against lipopolysaccharide‑induced myocardial injury. Experimental and Therapeutic Medicine, 16, 1825-1833. https://doi.org/10.3892/etm.2018.6386
MLA
Bao, J., Ye, C., Zheng, Z., Zhou, Z."Fmr1 protects cardiomyocytes against lipopolysaccharide‑induced myocardial injury". Experimental and Therapeutic Medicine 16.3 (2018): 1825-1833.
Chicago
Bao, J., Ye, C., Zheng, Z., Zhou, Z."Fmr1 protects cardiomyocytes against lipopolysaccharide‑induced myocardial injury". Experimental and Therapeutic Medicine 16, no. 3 (2018): 1825-1833. https://doi.org/10.3892/etm.2018.6386
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team