The 4‑hydroxynonenal mediated oxidative damage of blood proteins and lipids involves secondary lipid peroxidation reactions

Mustafa,Ayman G.; Alfaqih,Mahmoud A.; Al‑Shboul,Othman

doi:10.3892/etm.2018.6419

The 4‑hydroxynonenal mediated oxidative damage of blood proteins and lipids involves secondary lipid peroxidation reactions

Authors:
- Ayman G. Mustafa
- Mahmoud A. Alfaqih
- Othman Al‑Shboul
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Affiliations: Department of Basic Medical Sciences, School of Medicine, Jordan University of Science and Technology, Irbid 22110, Jordan, Department of Physiology and Biochemistry, School of Medicine, Jordan University of Science and Technology, Irbid 22110, Jordan
Published online on: July 6, 2018 https://doi.org/10.3892/etm.2018.6419
Pages: 2132-2137

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Abstract

Lipid peroxidation is associated with several metabolic diseases. Lipid peroxidation causes cellular damage through reactive aldehyde species such as 4‑hydroxyonenal (4‑HNE). The exact mechanism(s) by which 4‑HNE causes damage in the intravascular compartment is not yet exactly understood. Using an in vitro system, the damage induced by 4‑HNE on the blood was investigated by measuring protein carbonyl groups and thiobarbituric acid reactive substances (TBARS) following 4‑HNE treatment. The findings demonstrated that treatment with 4‑HNE increased the carbonylation of protein and the formation of TBARS in the blood plasma. It was also tested whether phenelzine, a scavenger of aldehyde species, or U‑83836E, a scavenger of lipid peroxy radicals, attenuated the damage caused by 4‑HNE. It was demonstrated that phenelzine or U‑83836E both mitigated the effects of 4‑HNE on the proteins and the lipids of the blood plasma. The findings of the current study suggest that phenelzine, U‑83836E or functionally similar therapeutics may prevent or treat diseases that involve an increased production of 4‑HNE in the intravascular compartment.

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