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HOXD10 silencing suppresses human fibroblast‑like synoviocyte migration in rheumatoid arthritis via downregulation of the p38/JNK pathway

  • Authors:
    • Luan Luan
    • Yingying Ma
    • Lihua Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Rheumatology, Jining No. 1 People's Hospital, Jining, Shandong 272000, P.R. China
    Copyright: © Luan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1621-1628
    |
    Published online on: July 9, 2018
       https://doi.org/10.3892/etm.2018.6432
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Abstract

Homeobox D10 (HOXD10) belongs to the human homeobox (HOX) gene family, and the homologous protein encoded by HOX primarily controls cell differentiation and morphogenesis during embryonic development. The current study aimed to explore the roles and mechanisms of HOXD10 in the migration of human fibroblast‑like synoviocytes in rheumatoid arthritis (RAFLS). Cell counting kit‑8, cell migration and wound healing assays were performed to examine the cell viability and migration, respectively. Western blot and reverse transcription‑quantitative polymerase chain reaction assays were used to evaluate the association between mRNA and protein expression levels. The results revealed HOXD10 expression was upregulated in tissues from patients with RA. HOXD10 silencing inhibited the viability of RAFLS. In addition, HOXD10 silencing suppressed the migration of RAFLS through modulating the expression of cadherin‑11, N‑cadherin, E‑cadherin, vimentin, zonula occludens‑1, integrinβ1 and paxillin. In conclusion, HOXD10 silencing downregulates the p38/c‑Jun N‑terminal kinase signaling pathway, which in turn may suppress the migration of RAFLS.
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Copy and paste a formatted citation
Spandidos Publications style
Luan L, Ma Y and Zhang L: HOXD10 silencing suppresses human fibroblast‑like synoviocyte migration in rheumatoid arthritis via downregulation of the p38/JNK pathway. Exp Ther Med 16: 1621-1628, 2018.
APA
Luan, L., Ma, Y., & Zhang, L. (2018). HOXD10 silencing suppresses human fibroblast‑like synoviocyte migration in rheumatoid arthritis via downregulation of the p38/JNK pathway. Experimental and Therapeutic Medicine, 16, 1621-1628. https://doi.org/10.3892/etm.2018.6432
MLA
Luan, L., Ma, Y., Zhang, L."HOXD10 silencing suppresses human fibroblast‑like synoviocyte migration in rheumatoid arthritis via downregulation of the p38/JNK pathway". Experimental and Therapeutic Medicine 16.3 (2018): 1621-1628.
Chicago
Luan, L., Ma, Y., Zhang, L."HOXD10 silencing suppresses human fibroblast‑like synoviocyte migration in rheumatoid arthritis via downregulation of the p38/JNK pathway". Experimental and Therapeutic Medicine 16, no. 3 (2018): 1621-1628. https://doi.org/10.3892/etm.2018.6432
Copy and paste a formatted citation
x
Spandidos Publications style
Luan L, Ma Y and Zhang L: HOXD10 silencing suppresses human fibroblast‑like synoviocyte migration in rheumatoid arthritis via downregulation of the p38/JNK pathway. Exp Ther Med 16: 1621-1628, 2018.
APA
Luan, L., Ma, Y., & Zhang, L. (2018). HOXD10 silencing suppresses human fibroblast‑like synoviocyte migration in rheumatoid arthritis via downregulation of the p38/JNK pathway. Experimental and Therapeutic Medicine, 16, 1621-1628. https://doi.org/10.3892/etm.2018.6432
MLA
Luan, L., Ma, Y., Zhang, L."HOXD10 silencing suppresses human fibroblast‑like synoviocyte migration in rheumatoid arthritis via downregulation of the p38/JNK pathway". Experimental and Therapeutic Medicine 16.3 (2018): 1621-1628.
Chicago
Luan, L., Ma, Y., Zhang, L."HOXD10 silencing suppresses human fibroblast‑like synoviocyte migration in rheumatoid arthritis via downregulation of the p38/JNK pathway". Experimental and Therapeutic Medicine 16, no. 3 (2018): 1621-1628. https://doi.org/10.3892/etm.2018.6432
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