Effect of JAK2/STAT3 signaling pathway on liver injury associated with severe acute pancreatitis in rats

  • Authors:
    • Minli Li
    • Xiaohua Zhang
    • Bin Wang
    • Xiaobing Xu
    • Xiaowei Wu
    • Meixia Guo
    • Fangyu Wang
  • View Affiliations

  • Published online on: July 9, 2018     https://doi.org/10.3892/etm.2018.6433
  • Pages: 2013-2021
  • Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Janus kinase/signal transducers and activators of transcription (JAK/STAT) signaling constitutes one of the major pathways for cytokine signal transduction. However, the role of the JAK2/STAT3 pathway in liver injury during severe acute pancreatitis (SAP) remains unclear. The aim of this study was to investigate the role of the JAK2/STAT3 signaling pathway in liver injury after SAP. In the present study 64 male Sprague‑Dawley rats were randomly divided into four groups: Control, AG490 (inhibition of JAK2), SAP and SAP with AG490. SAP was induced by retrograde infusion of 4% sodium taurocholate into the biliopancreatic duct. The activities of amylase (AMY) and liver enzymes were measured in serum. Livers and pancreas were isolated for measurements of histological damage. Blood and liver samples were taken for the measurement of TNF‑α, IL‑6 and IL‑18 concentrations. The expression levels of JAK2 and STAT3 in liver tissue were detected by immunohistochemical staining and western blotting. The results demonstrated that amylase and liver enzymes were higher in the SAP groups compared with the control, AG490 and AG490‑treated groups. The serum levels of TNF‑α, IL‑6 and IL‑18 were effectively increased in the SAP groups, whereas they were reduced by AG490. Interestingly, JAK2 and STAT3 protein expression levels were significantly increased following induction of SAP and were significantly decreased in the AG490‑pretreated groups. Administration of AG490 decreased the activity of pro‑inflammatory cytokines and significantly attenuated SAP associated‑liver injury in the rats. These results suggested that the mechanism may relate to the inhibition of TNF‑α, IL‑6 and IL‑18, and inhibiting excessive JAK2 and STAT3 activation, and may play a crucial role in the liver injury associated with SAP.
View Figures
View References

Related Articles

Journal Cover

September-2018
Volume 16 Issue 3

Print ISSN: 1792-0981
Online ISSN:1792-1015

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Li M, Zhang X, Wang B, Xu X, Wu X, Guo M and Wang F: Effect of JAK2/STAT3 signaling pathway on liver injury associated with severe acute pancreatitis in rats. Exp Ther Med 16: 2013-2021, 2018
APA
Li, M., Zhang, X., Wang, B., Xu, X., Wu, X., Guo, M., & Wang, F. (2018). Effect of JAK2/STAT3 signaling pathway on liver injury associated with severe acute pancreatitis in rats. Experimental and Therapeutic Medicine, 16, 2013-2021. https://doi.org/10.3892/etm.2018.6433
MLA
Li, M., Zhang, X., Wang, B., Xu, X., Wu, X., Guo, M., Wang, F."Effect of JAK2/STAT3 signaling pathway on liver injury associated with severe acute pancreatitis in rats". Experimental and Therapeutic Medicine 16.3 (2018): 2013-2021.
Chicago
Li, M., Zhang, X., Wang, B., Xu, X., Wu, X., Guo, M., Wang, F."Effect of JAK2/STAT3 signaling pathway on liver injury associated with severe acute pancreatitis in rats". Experimental and Therapeutic Medicine 16, no. 3 (2018): 2013-2021. https://doi.org/10.3892/etm.2018.6433