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Article Open Access

Effects of UV on apoptotic factors in lens epithelial cells of an animal model

  • Authors:
    • Juling Lv
    • Yiqiao Xing
  • View Affiliations / Copyright

    Affiliations: Eye Center, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, P.R. China
    Copyright: © Lv et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2309-2312
    |
    Published online on: July 10, 2018
       https://doi.org/10.3892/etm.2018.6435
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Abstract

Effect of ultraviolet (UV) on the apoptotic factors p53, Bax and Bcl-2 in lens epithelial cells (LECs) was explored. Sixty SD rats were randomly divided into control group (n=12) and model group (n=48). Rats in model group were used to construct UV radiation eyeball models. Twelve rats in model group and 3 rats in control group were sacrificed at day 1, 3, 5 and 7, and the expression of p53, Bax and Bcl-2 in LECs was detected by RT-qPCR. There was no significant difference in p53 expression between model and control group on day 1 (P>0.05), while expression level of p53 was significantly higher in model than in control group at day 3, 5 and 7 (P<0.05), and the highest expression level was found on day 7 (P<0.05). Relative expression level of Bax in model group was significantly higher than that in control group (P<0.05), and the highest expression level was found on day 7 (P<0.05). On day 1, expression level of Bcl-2 in model group was significantly lower than that in control group (P<0.05), but it began to rise on day 3 but did not differ significantly from the control group (P>0.05), and was significantly higher in model than in control group on day 5 and 7 (P<0.05), and the highest expression level was observed on day 7 (P<0.05). Linear correlation analysis showed that there was a positive correlation between p53, Bax and Bcl-2 expression and UV exposure time in LEC (r=0.82, 0.90, 0.95, P<0.05). Expression levels of p53, Bax, and Bcl-2 in LEC of rats are positively correlated with the time after UV irradiation, suggesting that UV could induce apoptosis of LEC by promoting p53, Bax and Bcl-2 expression.
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Copy and paste a formatted citation
Spandidos Publications style
Lv J and Xing Y: Effects of UV on apoptotic factors in lens epithelial cells of an animal model. Exp Ther Med 16: 2309-2312, 2018.
APA
Lv, J., & Xing, Y. (2018). Effects of UV on apoptotic factors in lens epithelial cells of an animal model. Experimental and Therapeutic Medicine, 16, 2309-2312. https://doi.org/10.3892/etm.2018.6435
MLA
Lv, J., Xing, Y."Effects of UV on apoptotic factors in lens epithelial cells of an animal model". Experimental and Therapeutic Medicine 16.3 (2018): 2309-2312.
Chicago
Lv, J., Xing, Y."Effects of UV on apoptotic factors in lens epithelial cells of an animal model". Experimental and Therapeutic Medicine 16, no. 3 (2018): 2309-2312. https://doi.org/10.3892/etm.2018.6435
Copy and paste a formatted citation
x
Spandidos Publications style
Lv J and Xing Y: Effects of UV on apoptotic factors in lens epithelial cells of an animal model. Exp Ther Med 16: 2309-2312, 2018.
APA
Lv, J., & Xing, Y. (2018). Effects of UV on apoptotic factors in lens epithelial cells of an animal model. Experimental and Therapeutic Medicine, 16, 2309-2312. https://doi.org/10.3892/etm.2018.6435
MLA
Lv, J., Xing, Y."Effects of UV on apoptotic factors in lens epithelial cells of an animal model". Experimental and Therapeutic Medicine 16.3 (2018): 2309-2312.
Chicago
Lv, J., Xing, Y."Effects of UV on apoptotic factors in lens epithelial cells of an animal model". Experimental and Therapeutic Medicine 16, no. 3 (2018): 2309-2312. https://doi.org/10.3892/etm.2018.6435
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