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In vitro culture and biological properties of broiler adipose‑derived stem cells

  • Authors:
    • Tengfei Lu
    • Wenhua Pei
    • Kunfu Wang
    • Shuang Zhang
    • Fenghao Chen
    • Yangnan Wu
    • Weijun Guan
  • View Affiliations / Copyright

    Affiliations: Department of Animal Genetic Resources, Institute of Animal Science, Chinese Academy of Agricultural Science, Beijing 100193, P.R. China, College of Wildlife Resources, Northeast Forestry University, Harbin, Heilongjiang 150040, P.R. China, Scientific Experiment Research Center, Harbin Sport University, Harbin, Heilongjiang 150008, P.R. China, College of Human Movement Science, Harbin Sport University, Harbin, Heilongjiang 150008, P.R. China
    Copyright: © Lu et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 4.0].
  • Pages: 2399-2407
    |
    Published online on: July 16, 2018
       https://doi.org/10.3892/etm.2018.6445
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Abstract

In the past 10 years, adipose‑derived stem cells (ADSCs) have been applied due to their pluripotency. Experimental tissues have been frequently obtained from mammals, including rabbits, mice and humans, but rarely from broilers, Gallus gallus domesticus. In the present study, ADSCs were obtained from 20‑day‑old broiler embryos. Primary ADSCs were sub‑cultured to passage 37 in vitro. The surface markers of ADSCs, namely CD29, CD31, CD44, CD71 and CD73, were detected by reverse transcription polymerase chain reaction and immunofluorescence assays. The result indicated that CD29, CD44, CD71 and CD73 were expressed on the surface of cells at various passages, but not CD31. The growth curve of cells at the different passages had a typical sigmoidal shape. Furthermore, ADSCs were successfully induced to differentiate into osteoblasts, adipocytes and hepatocyte‑like cells. The results denote that the ADSCs isolated from broilers have similar biological properties to those of ADSCs obtained from other animals. The present study provided a theoretical and experimental foundation for the use of poultry as a source of stem cells, and laid a foundation for adipose tissue engineering and strategies in regenerative medicine.
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Copy and paste a formatted citation
Spandidos Publications style
Lu T, Pei W, Wang K, Zhang S, Chen F, Wu Y and Guan W: In vitro culture and biological properties of broiler adipose‑derived stem cells. Exp Ther Med 16: 2399-2407, 2018.
APA
Lu, T., Pei, W., Wang, K., Zhang, S., Chen, F., Wu, Y., & Guan, W. (2018). In vitro culture and biological properties of broiler adipose‑derived stem cells. Experimental and Therapeutic Medicine, 16, 2399-2407. https://doi.org/10.3892/etm.2018.6445
MLA
Lu, T., Pei, W., Wang, K., Zhang, S., Chen, F., Wu, Y., Guan, W."In vitro culture and biological properties of broiler adipose‑derived stem cells". Experimental and Therapeutic Medicine 16.3 (2018): 2399-2407.
Chicago
Lu, T., Pei, W., Wang, K., Zhang, S., Chen, F., Wu, Y., Guan, W."In vitro culture and biological properties of broiler adipose‑derived stem cells". Experimental and Therapeutic Medicine 16, no. 3 (2018): 2399-2407. https://doi.org/10.3892/etm.2018.6445
Copy and paste a formatted citation
x
Spandidos Publications style
Lu T, Pei W, Wang K, Zhang S, Chen F, Wu Y and Guan W: In vitro culture and biological properties of broiler adipose‑derived stem cells. Exp Ther Med 16: 2399-2407, 2018.
APA
Lu, T., Pei, W., Wang, K., Zhang, S., Chen, F., Wu, Y., & Guan, W. (2018). In vitro culture and biological properties of broiler adipose‑derived stem cells. Experimental and Therapeutic Medicine, 16, 2399-2407. https://doi.org/10.3892/etm.2018.6445
MLA
Lu, T., Pei, W., Wang, K., Zhang, S., Chen, F., Wu, Y., Guan, W."In vitro culture and biological properties of broiler adipose‑derived stem cells". Experimental and Therapeutic Medicine 16.3 (2018): 2399-2407.
Chicago
Lu, T., Pei, W., Wang, K., Zhang, S., Chen, F., Wu, Y., Guan, W."In vitro culture and biological properties of broiler adipose‑derived stem cells". Experimental and Therapeutic Medicine 16, no. 3 (2018): 2399-2407. https://doi.org/10.3892/etm.2018.6445
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