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Triptolide protects podocytes via autophagy in immunoglobulin A nephropathy

  • Authors:
    • Shikai Liang
    • Juan Jin
    • Xiaogang Shen
    • Xinxin Jiang
    • Yiwen Li
    • Qiang He
  • View Affiliations / Copyright

    Affiliations: Department of Nephrology, Zhejiang Provincial People's Hospital, Hangzhou, Zhejiang 310014, P.R. China
    Copyright: © Liang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2275-2280
    |
    Published online on: July 19, 2018
       https://doi.org/10.3892/etm.2018.6480
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Abstract

Triptolide is often used to treat patients with immunoglobulin A nephropathy (IgAN), especially in Asia. However, its detailed mechanism remains unclear. In vitro experiments were conducted with podocytes exposed to aggregated IgA (aIgA)‑MSC1097‑conditioned media. A total of four groups were compared in this study: A control group (CON); a healthy supernatant group (HEAs); an IgAN supernatant group (IgANs); and a triptolide group (TRI). First, aggregated IgA1 (aIgA1) was generated by heating monomeric IgA1 (mIgA1) from IgAN patients or healthy subjects. Next, the conditioned supernatant of MSC‑1097 cells cultured with aIgA1 (100 mg/l) from IgAN patients (IgANs) or healthy subjects (HEAs) or without aIgA1 (CON) were harvested and used to incubate MPC5 cells. MPC5 cells in the TRI group were cultured with triptolide (10 ng/ml) and conditioned media from MSC‑1097 cells cultured with aIgA1 from IgAN patients. After 24 h of treatment, MPC5 cells were collected to measure autophagy‑related protein levels, including microtubule‑associated protein light chain 3 (LC3), p62, cluster of differentiation (CD)63, phosphorylated‑protein kinase B (Akt), Akt, p‑mammalian target of rapamycin (mTOR), and mTOR, via western blotting, immunofluorescence or both, and to determine apoptosis by flow cytometry. All the results showed no difference between the CON and the HEAs. Compared to the CON and the HEAs, MPC5 cells in the IgANs group showed reduced autophagy, which was presented as decreased levels of LC3‑II and CD63, as well as accumulation of p62, and an increased podocyte apoptosis rate. This was partly rescued by the addition of triptolide. Moreover, the p‑mTOR/mTOR ratio increased in the IgANs group and decreased in the TRI group. Therefore, these results suggest that triptolide protects podocyte autophagy in IgAN patients.
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1 

Berger J and Hinglais N: Intercapillary deposits of IgA-IgG. J Urol Nephrol (Paris). 74:694–695. 1968.(In French). PubMed/NCBI

2 

Gharavi AG, Kiryluk K, Choi M, Li Y, Hou P, Xie J, Sanna-Cherchi S, Men CJ, Julian BA, Wyatt RJ, et al: Genome-wide association study identifies susceptibility loci for IgA nephropathy. Nat Genet. 43:321–327. 2011. View Article : Google Scholar : PubMed/NCBI

3 

Wang C, Tang Y, Peng H, Ye ZC, Chen ZJ, Yu XQ and Lou TQ: Supernatant of cultured mesangial cells with IgA1 from IgA nephropathy induces apoptosis of podocyte. Chin J Nephrol. 24:387–391. 2008.

4 

Wang C, Ye Z, Peng H, Tang H, Liu X, Chen Z, Yu X and Lou T: Effect of aggregated immunoglobulin A1 from immunoglobulin A nephropathy patients on nephrin expression in podocytes. Nephrology (Carlton). 14:213–218. 2009. View Article : Google Scholar : PubMed/NCBI

5 

Zhu L, Zhang Q, Shi S, Liu L, Lv J and Zhang H: Synergistic effect of mesangial cell-induced CXCL1 and TGF-β1 in promoting podocyte loss in IgA nephropathy. PLoS One. 8:e734252013. View Article : Google Scholar : PubMed/NCBI

6 

Tewari R, Nada R, Rayat CS, Boruah D, Dudeja P, Joshi K and Sakhuja V: Correlation of proteinuria with podocyte foot process effacement in IgA nephropathy: An ultrastructural study. Ultrastruct Pathol. 39:147–151. 2015. View Article : Google Scholar : PubMed/NCBI

7 

Shen P, Shen J, Li W and He L: Urinary podocyte can be an indicator for the pathogenetic condition of patients with IgA nephropathy. Clin Lab. 60:1709–1715. 2014. View Article : Google Scholar : PubMed/NCBI

8 

Yasuda-Yamahara M, Kume S, Tagawa A, Maegawa H and Uzu T: Emerging role of podocyte autophagy in the progression of diabetic nephropathy. Autophagy. 11:2385–2386. 2015. View Article : Google Scholar : PubMed/NCBI

9 

Hartleben B, Gödel M, Meyer-Schwesinger C, Liu S, Ulrich T, Köbler S, Wiech T, Grahammer F, Arnold SJ, Lindenmeyer MT, et al: Autophagy influences glomerular disease susceptibility and maintains podocyte homeostasis in aging mice. J Clin Invest. 120:1084–1096. 2010. View Article : Google Scholar : PubMed/NCBI

10 

Li XJ, Jiang ZZ and Zhang LY: Triptolide: Progress on research in pharmacodynamics and toxicology. J Ethnopharmacol. 155:67–79. 2014. View Article : Google Scholar : PubMed/NCBI

11 

Liang S, Jin J, Lin B, Gong J, Li Y and He Q: Rapamycin induces autophagy and reduces the apoptosis of podocytes under a stimulated condition of immunoglobulin A nephropathy. Kidney Blood Press Res. 42:177–187. 2017. View Article : Google Scholar : PubMed/NCBI

12 

Dai Q, Liu J, Du YL, Hao X, Ying J, Tan Y, He LQ, Wang WM and Chen N: Histone deacetylase inhibitors attenuate P-aIgA1-induced cell proliferation and extracellular matrix synthesis in human renal mesangial cells in vitro. Acta Pharmacol Sin. 37:228–234. 2016. View Article : Google Scholar : PubMed/NCBI

13 

Yang Q, Sun M, Chen Y, Lu Y, Ye Y, Song H, Xu X, Shi S and Wang J: Triptolide protects podocytes from TGF-β-induced injury by preventing miR-30 downregulation. Am J Transl Res. 9:5150–5159. 2017.PubMed/NCBI

14 

Liu ZH: Nephrology in China. Nat Rev Nephrol. 9:523–528. 2013. View Article : Google Scholar : PubMed/NCBI

15 

Wyatt RJ and Julian BA: IgA nephropathy. N Engl J Med. 368:2402–2414. 2013. View Article : Google Scholar : PubMed/NCBI

16 

Lin M, Du L, Brandtzaeg P and Pan-Hammarström Q: IgA subclass switch recombination in human mucosal and systemic immune compartments. Mucosal Immunol. 7:511–520. 2014. View Article : Google Scholar : PubMed/NCBI

17 

Graziose R, Lila MA and Raskin I: Merging traditional Chinese medicine with modern drug discovery technologies to find novel drugs and functional foods. Curr Drug Discov Technol. 7:2–12. 2010. View Article : Google Scholar : PubMed/NCBI

18 

Liu Q: Triptolide and its expanding multiple pharmacological functions. Int Immunopharmacol. 11:377–383. 2011. View Article : Google Scholar : PubMed/NCBI

19 

He L, Peng X, Liu G, Tang C, Liu H, Liu F, Zhou H and Peng Y: Anti-inflammatory effects of triptolide on IgA nephropathy in rats. Immunopharmacol Immunotoxicol. 37:421–427. 2015. View Article : Google Scholar : PubMed/NCBI

20 

Zheng CX, Chen ZH, Zeng CH, Qin WS, Li LS and Liu ZH: Triptolide protects podocytes from puromycin aminonucleoside induced injury in vivo and in vitro. Kidney Int. 74:596–612. 2008. View Article : Google Scholar : PubMed/NCBI

21 

Wang YG, Sun W and Zhen YJ: Preventive effect of multi-glycoside of Tripterygium wilfordii Hook. f. on proteinuria and mesangial injury in experimental mesangial proliferative glomerulonephritis. Zhongguo Zhong Xi Yi Jie He Za Zhi. 25:817–821. 2005.(In Chinese).

22 

Ma R, Liu L, Liu X, Wang Y, Jiang W and Xu L: Triptolide markedly attenuates albuminuria and podocyte injury in an animal model of diabetic nephropathy. Exp Ther Med. 6:649–656. 2013. View Article : Google Scholar : PubMed/NCBI

23 

Jiang CB, Wei MG, Tu Y, Zhu H, Li CQ, Jing WM and Sun W: Triptolide attenuates podocyte injury by regulating expression of miRNA-344b-3p and miRNA-30b-3p in rats with adriamycin-induced nephropathy. Evid Based Complement Alternat Med. 2015:1078142015. View Article : Google Scholar : PubMed/NCBI

24 

Sato S, Yanagihara T, Ghazizadeh M, Ishizaki M, Adachi A, Sasaki Y, Igarashi T and Fukunaga Y: Correlation of autophagy type in podocytes with histopathological diagnosis of IgA nephropathy. Pathobiology. 76:221–226. 2009. View Article : Google Scholar : PubMed/NCBI

25 

Liang S, Jin J, Gong J, Lin B, Li Y and He Q: How many podocyte autophagosomes are there in immunoglobulin A nephropathy and idiopathic membranous nephropathy? Int Urol Nephrol. 48:2109–2114. 2016. View Article : Google Scholar : PubMed/NCBI

26 

Yang Y, Gao K, Hu Z, Li W, Davies H, Ling S, Rudd JA and Fang M: Autophagy upregulation and apoptosis downregulation in DAHP and triptolide treated cerebral ischemia. Mediators Inflamm 2015. 1201982015.

27 

Hu G, Gong X, Wang L, Liu M, Liu Y, Fu X, Wang W, Zhang T and Wang X: Triptolide promotes the clearance of α-synuclein by enhancing autophagy in neuronal cells. Mol Neurobiol. 54:2361–2372. 2017. View Article : Google Scholar : PubMed/NCBI

28 

Park JH, Kim KP, Ko JJ and Park KS: PI3K/Akt/mTOR activation by suppression of ELK3 mediates chemosensitivity of MDA-MB-231 cells to doxorubicin by inhibiting autophagy. Biochem Biophys Res Commun. 477:277–282. 2016. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Liang S, Jin J, Shen X, Jiang X, Li Y and He Q: Triptolide protects podocytes via autophagy in immunoglobulin A nephropathy. Exp Ther Med 16: 2275-2280, 2018.
APA
Liang, S., Jin, J., Shen, X., Jiang, X., Li, Y., & He, Q. (2018). Triptolide protects podocytes via autophagy in immunoglobulin A nephropathy. Experimental and Therapeutic Medicine, 16, 2275-2280. https://doi.org/10.3892/etm.2018.6480
MLA
Liang, S., Jin, J., Shen, X., Jiang, X., Li, Y., He, Q."Triptolide protects podocytes via autophagy in immunoglobulin A nephropathy". Experimental and Therapeutic Medicine 16.3 (2018): 2275-2280.
Chicago
Liang, S., Jin, J., Shen, X., Jiang, X., Li, Y., He, Q."Triptolide protects podocytes via autophagy in immunoglobulin A nephropathy". Experimental and Therapeutic Medicine 16, no. 3 (2018): 2275-2280. https://doi.org/10.3892/etm.2018.6480
Copy and paste a formatted citation
x
Spandidos Publications style
Liang S, Jin J, Shen X, Jiang X, Li Y and He Q: Triptolide protects podocytes via autophagy in immunoglobulin A nephropathy. Exp Ther Med 16: 2275-2280, 2018.
APA
Liang, S., Jin, J., Shen, X., Jiang, X., Li, Y., & He, Q. (2018). Triptolide protects podocytes via autophagy in immunoglobulin A nephropathy. Experimental and Therapeutic Medicine, 16, 2275-2280. https://doi.org/10.3892/etm.2018.6480
MLA
Liang, S., Jin, J., Shen, X., Jiang, X., Li, Y., He, Q."Triptolide protects podocytes via autophagy in immunoglobulin A nephropathy". Experimental and Therapeutic Medicine 16.3 (2018): 2275-2280.
Chicago
Liang, S., Jin, J., Shen, X., Jiang, X., Li, Y., He, Q."Triptolide protects podocytes via autophagy in immunoglobulin A nephropathy". Experimental and Therapeutic Medicine 16, no. 3 (2018): 2275-2280. https://doi.org/10.3892/etm.2018.6480
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