Open Access

miRNA‑218 regulates the proliferation and apoptosis of cervical cancer cells via targeting Gli3

  • Authors:
    • Jing Zhang
    • Shengze Li
    • Yanhua Li
    • Hongli Liu
    • Yuan Zhang
    • Qingsong Zhang
  • View Affiliations

  • Published online on: July 20, 2018     https://doi.org/10.3892/etm.2018.6491
  • Pages: 2433-2441
  • Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Cervical cancer (CC) is one of the most malignant tumors that affect women. Recent studies have reported that microRNAs (miRs) serve important roles in CC. The aim of the present study was to investigate the role of miR‑218 in CC and to verify its underlying mechanism. The results of reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) revealed that miR‑218 was dramatically downregulated in CC tissues and cell lines. Furthermore, the expression of Gli3 and Ki67 was measured using RT‑qPCR and the results revealed that levels of these proteins were negatively correlated with miR‑218 in CC tissues. The protein expression levels were determined by western blotting. Then SiHa cell line was used to investigate the mechanism of CC. Following cell transfection, cell apoptosis and cycle analyses were performed using the flow cytometry. The results revealed that miR‑218 overexpression significantly inhibited cell proliferation, apoptosis and cell cycle. Additionally, luciferase reporter assay revealed that Gli3 may be a novel and direct target of miR‑218 in CC. Taken together, the results of the present study suggest that miR‑218 overexpression or Gli3 knockdown may have potential as therapeutic strategies for the treatment of CC.
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September-2018
Volume 16 Issue 3

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Zhang J, Li S, Li Y, Liu H, Zhang Y and Zhang Q: miRNA‑218 regulates the proliferation and apoptosis of cervical cancer cells via targeting Gli3. Exp Ther Med 16: 2433-2441, 2018
APA
Zhang, J., Li, S., Li, Y., Liu, H., Zhang, Y., & Zhang, Q. (2018). miRNA‑218 regulates the proliferation and apoptosis of cervical cancer cells via targeting Gli3. Experimental and Therapeutic Medicine, 16, 2433-2441. https://doi.org/10.3892/etm.2018.6491
MLA
Zhang, J., Li, S., Li, Y., Liu, H., Zhang, Y., Zhang, Q."miRNA‑218 regulates the proliferation and apoptosis of cervical cancer cells via targeting Gli3". Experimental and Therapeutic Medicine 16.3 (2018): 2433-2441.
Chicago
Zhang, J., Li, S., Li, Y., Liu, H., Zhang, Y., Zhang, Q."miRNA‑218 regulates the proliferation and apoptosis of cervical cancer cells via targeting Gli3". Experimental and Therapeutic Medicine 16, no. 3 (2018): 2433-2441. https://doi.org/10.3892/etm.2018.6491