Acetylpuerarin inhibits oxygen‑glucose deprivation‑induced neuroinflammation of rat primary astrocytes via the suppression of HIF‑1 signaling

Xiang,Yanxiao; Du,Pengchao; Zhang,Xiumei; Biswas,Siddhartha; Jiao,Guangjun; Liu,Haichun

doi:10.3892/etm.2018.6509

September-2018 Volume 16 Issue 3

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September-2018 Volume 16 Issue 3

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Article

Acetylpuerarin inhibits oxygen‑glucose deprivation‑induced neuroinflammation of rat primary astrocytes via the suppression of HIF‑1 signaling

Authors:
- Yanxiao Xiang
- Pengchao Du
- Xiumei Zhang
- Siddhartha Biswas
- Guangjun Jiao
- Haichun Liu
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Affiliations: Department of Clinical Pharmacy, Qilu Hospital of Shandong University, Jinan, Shandong 250012, P.R. China, College of Basic Medicine, Binzhou Medical University, Yantai, Shandong 264033, P.R. China, Department of Pharmacology, Shandong University School of Medicine, Jinan, Shandong 250012, P.R. China, Department of Hepatobiliary Surgery, Qilu Hospital of Shandong University, Jinan, Shandong 250012, P.R. China, Department of Orthopaedics, Qilu Hospital of Shandong University, Jinan, Shandong 250012, P.R. China
Pages: 2689-2695
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Published online on: July 23, 2018

https://doi.org/10.3892/etm.2018.6509
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Abstract

In the central nervous system (CNS), ischemic injury induced by inflammation associated with astrocytes serves an important role in physiological and pathological processes. Neuroinflammation leads to the release of pro‑inflammatory cytokines, including tumor necrosis factor‑α and interleukin‑1β. The aim of the present study was to investigate whether acetylpuerarin attenuates oxygen‑glucose deprivation (OGD)‑induced astrocyte inflammation and secretion of pro‑inflammatory cytokines via inhibiting hypoxia‑inducible factor‑1 (HIF‑1) activation and suppressing downstream primary astrocyte signaling in rats. The results demonstrated that acetylpuerarin attenuates astrocyte viability and induces morphological changes following OGD stress. Furthermore, acetylpuerarin suppresses the stimulation of HIF‑1α and nuclear factor (NF)‑κB signaling pathways, while attenuating the expression and secretion of pro‑inflammatory cytokines via HIF‑1 suppression in OGD‑induced astrocytes. These findings indicate that acetylpuerarin may attenuate OGD‑induced astrocyte damage and inflammation in rat primary astrocytes via suppressing HIF‑1 activation and NF‑κB signaling. These results suggest that acetylpuerarin regulates inflammation associated with astrocytes and may represent a novel therapeutic agent for the treatment of neuroinflammation in the CNS.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Acetylpuerarin inhibits oxygen‑glucose deprivation‑induced neuroinflammation of rat primary astrocytes via the suppression of HIF‑1 signaling

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